Correlation of TP53 Overexpression and Clinical Parameters with Five-Year Survival in Oral Squamous Cell Carcinoma Patients

Khan, Hamza Aijaz; Irfan, Omar; Samad, Adeel; Mirza, Yamna; Awan, M. S.

doi:10.7759/cureus.1401

Cited by 2 publications

(2 citation statements)

References 19 publications

(21 reference statements)

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“…We designed our panel to analyze the target hot spots of genes relevant for CNS tumors, NSCLCs, CRCs, thyroid nodules, melanomas, pancreatic lesions, oral squamous carcinomas, and GISTs according to updated guidelines and diagnostic/prognostic/predictive clinical needs [2,3,[5][6][7][8][9][10][11][14][15][16][17][18][19]24,43].…”

Section: Discussionmentioning

confidence: 99%

Molecular Diagnostic of Solid Tumor Using a Next Generation Sequencing Custom-Designed Multi-Gene Panel

et al. 2020

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Next generation sequencing (NGS) allows parallel sequencing of multiple genes at a very high depth of coverage. The need to analyze a variety of targets for diagnostic/prognostic/predictive purposes requires multi-gene characterization. Multi-gene panels are becoming standard approaches for the molecular analysis of solid lesions. We report a custom-designed 128 multi-gene panel engineered to cover the relevant targets in 22 oncogene/oncosuppressor genes for the analysis of the solid tumors most frequently subjected to routine genotyping. A total of 1695 solid tumors were analyzed for panel validation. The analytical sensitivity is 5%. Analytical validation: (i) Accuracy: sequencing results obtained using the multi-gene panel are concordant using two different NGS platforms and single-gene approach sequencing (100% of 83 cases); (ii) Precision: consistent results are obtained in the samples analyzed twice with the same platform (100% of 20 cases). Clinical validation: the frequency of mutations identified in different tumor types is consistent with the published literature. This custom-designed multi-gene panel allows to analyze with high sensitivity and throughput 22 oncogenes/oncosuppressor genes involved in diagnostic/prognostic/predictive characterization of central nervous system tumors, non-small-cell lung carcinomas, colorectal carcinomas, thyroid nodules, pancreatic lesions, melanoma, oral squamous carcinomas and gastrointestinal stromal tumors.

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Section: Discussionmentioning

confidence: 99%

Molecular Diagnostic of Solid Tumor Using a Next Generation Sequencing Custom-Designed Multi-Gene Panel

et al. 2020

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“…To determine the Cyclin D1 expression we used a more objective and inclusive method as depicted by Guy et al 2010. 14,15 Both nuclear and cytoplasmic staining was considered as positive. Cytoplasmic staining in absence of nuclear staining was considered negative.…”

Section: Assessment Of Stainingmentioning

confidence: 99%

Expression of Cyclin D1 in oral squamous cell carcinoma and its correlation with histopathological differentiation

Mishra¹,

Hota²,

Giri³

et al. 2020

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Background: Cyclin D1 is a vital protein that has a widespread role in cell cycle regulations, providing control over G1 to S Phase transition and governing cell proliferation rates.Cyclin D1 overexpression has been reported in variety of tumors. Present study was carried out to the study the expression of cyclin D1 and its association with histopathological differentiation and stage of oral squamous cell carcinoma. Materials and Methods: 48 formalin fixed paraffin embedded tissue blocks of biopsy specimens of oral squamous cell carcinoma were immunohistochemically evaluated for expression of cyclinD1. Result: CyclinD1 expression was seen in 98.5% (46) cases of OSCC. It did not correlate with site and staging. CyclinD1 expression was seen in 94.6% of well differentiated tumor,100% in moderately differentiated and 100% in poorly differentiated oral squamous cell carcinoma, but it was not statistically significant(p=0.065). Conclusion: Relatively higher frequency of Cyclin D1 immuno-reactivity observed in patients with less differentiated tumours suggest inverse correlation of Cyclin D1 expression with histological differentiation of tumour.

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Correlation of TP53 Overexpression and Clinical Parameters with Five-Year Survival in Oral Squamous Cell Carcinoma Patients

Cited by 2 publications

References 19 publications

Molecular Diagnostic of Solid Tumor Using a Next Generation Sequencing Custom-Designed Multi-Gene Panel

Molecular Diagnostic of Solid Tumor Using a Next Generation Sequencing Custom-Designed Multi-Gene Panel

Expression of Cyclin D1 in oral squamous cell carcinoma and its correlation with histopathological differentiation

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