Abstract:Adenovirus-infected tissue culture cells have been investigated by a variety of methods, including light and electron microscopy and cytochemical and fluorescent antibody techniques (1-7). The results of these studies have shown that adenoviruses produce characteristic sequential alterations in host-cell nuclei, and have demonstrated that certain of the structures which develop contain virus-like particles, deoxyribonucleic acid (DNA), and type-specific viral antigen. This evidence indicates that the intranucl… Show more
“…Light and electron microscopic observations of infected IgH-2 cells showed a time dependent development of nuclear alterations. These findings were similar to those obtained with mammalian and avian adenoviruses in correlation of production of the viruses [1,5,6,10,15,[23][24][25][26][27]. Formation of intranuclear inclusion bodies and nuclear vacuolization of infected IgH-2 cells were dominant.…”
A virus isolated from the internal organs of a moribund corn snake (Elaphe guttata) replicated in reptilian cell cultures (IgH-2, TH-1 cells) between 10 and 30 degrees C. Highest infectivity titers of 10(5.5) TCID50/ml were obtained in IgH-2 cells at 25 degrees C. Infected IgH-2 cells showed the development of three morphologically different intranuclear inclusion bodies. During viral assembly the particles formed typical crystalline aggregates in the nucleus. About 64 h after infection progressive desintegration of the nuclear membrane was evident and virus particles were released into the cytoplasm. Different fish cell lines (CLC, CHSE-214, BF-2, PG, RTG-2) were not capable of propagating the virus. The DNA containing agent proved to be stable at pH3, more or less at pH 12 and to treatment with chloroform, but it was rapidly inactivated at 56 degrees C. Electron microscopy revealed nonenveloped icosahedral particles with a diameter of 65-70 nm.
“…Light and electron microscopic observations of infected IgH-2 cells showed a time dependent development of nuclear alterations. These findings were similar to those obtained with mammalian and avian adenoviruses in correlation of production of the viruses [1,5,6,10,15,[23][24][25][26][27]. Formation of intranuclear inclusion bodies and nuclear vacuolization of infected IgH-2 cells were dominant.…”
A virus isolated from the internal organs of a moribund corn snake (Elaphe guttata) replicated in reptilian cell cultures (IgH-2, TH-1 cells) between 10 and 30 degrees C. Highest infectivity titers of 10(5.5) TCID50/ml were obtained in IgH-2 cells at 25 degrees C. Infected IgH-2 cells showed the development of three morphologically different intranuclear inclusion bodies. During viral assembly the particles formed typical crystalline aggregates in the nucleus. About 64 h after infection progressive desintegration of the nuclear membrane was evident and virus particles were released into the cytoplasm. Different fish cell lines (CLC, CHSE-214, BF-2, PG, RTG-2) were not capable of propagating the virus. The DNA containing agent proved to be stable at pH3, more or less at pH 12 and to treatment with chloroform, but it was rapidly inactivated at 56 degrees C. Electron microscopy revealed nonenveloped icosahedral particles with a diameter of 65-70 nm.
“…Through (Boyer, Denny, Miller & Ginsberg, 1960) in control cultures inoculated with fetal bovine serum in place of the test serum. Titres of 1/5 or greater were considered positive.…”
SUMMARYAntibodies neutralizing adenovirus type 5 were found in all of 50 pairs (100 %) of sera from patients with acute icteric infectious hepatitis. The incidence in sera from the general population was 57 %. No differences in mean titre or in proportion of positive sera were found in the same sera tested for complement-fixing antibodies to cytomegalovirus and for antibodies to rubella virus haemagglutinin. The results can be interpreted as supporting the involvement, either direct or indirect, of adenovirus in the aetiology of infectious hepatitis; but could also be due to a non-specific anamnestic enhancement of the production of antibody to adenovirus, or to coincidental infection with adenovirus and the agent of infectious hepatitis.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.