Correlation of Infectivity and Concanavalin A Agglutinability of Algae Exsymbiotic from <i>Paramecium bursaria</i>

Weis, Dale S.

doi:10.1111/j.1550-7408.1978.tb03905.x

Cited by 40 publications

(23 citation statements)

References 17 publications

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“…In this regard, PBCV-1 is especially promising because several investigators (ref. 12 We thank Myron Brakke, Les Lane, and R. L. Pardy for many useful discussions and Ellen Ball for making antiserum to HVCV-1. This paper is published with the approval ofthe Director as paper no.…”

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confidence: 99%

Viruses of symbiotic Chlorella -like algae isolated from Paramecium bursaria and Hydra viridis

Etten

Meints

Kuczmarski

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

101

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We previously reported that isolation of symbiotic Chloreila-like algae from the Florida strain ofHydra viridis induced replication of a virus (designated HVCV-1) in the algae. We now report that isolation ofsymbiotic Chlorella-like algae from four other sources of green hydra and one source ofthe protozoan Paramecium bursaria also induced virus synthesis. Algae from one of these hydra contained a virus identical to HVCV-1 (based on its rate of sedimentation, buoyant density, reaction to HVCV-1 antiserum, and DNA restriction fragments) whereas algae from the other three hydra contained. another similar, but distinct, virus (designated HVCV-2). The virus from the paramecium algae (designated PBCV-1) was distinct from both HVCV-1 and HVCV-2. The symbiotic algae in the hydra could also be distinguished ultrastructurally. Chloroplasts of both algae that produced HVCV-1 lacked a pyrenoid whereas chloroplasts of the other three symbiotic algae contained pyrenoids. Since all symbiotic eukaryotic algae we have examined have had virus, a potential viral role in symbiosis is suggested.

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mentioning

confidence: 99%

Viruses of symbiotic Chlorella -like algae isolated from Paramecium bursaria and Hydra viridis

Etten

Meints

Kuczmarski

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

101

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“…Con A agglutination titers Jvrre detrrniincd on log-phase algae cultivated also for sugar release and infectivity studies. Details of the preparation of cell suspensions for Con X treatment \<ere described previously ( 14).…”

Section: Methodsmentioning

confidence: 99%

“…T h e present report is concerned with the possibility that specific sugar release by algae during their ingestion by an aposymbiotic ciliate may servr as a signal to the potential host resulting in the establishment of the algae as symbiotes. I n a previous report (14) a comparison was made between the response of symbiotic algae to the lectin Concanavalin A (Con A ) and the infectivity of the algae for aposymbiotic ciliates. It was found that infective algal strains were also relatively resistant to agglutination in the presence of Con A. Resistancr and sensitivity to agglutination were, however, about equally represented among the noninfective strains.…”

mentioning

confidence: 99%

Correlation of Sugar Release and Concanavalin A Agglutinability with Infectivity of Symbiotic Algae from Paramecium bursaria for Aposymbiotic P. bursaria

Weis

1979

The Journal of Protozoology

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Toxoplasma REPRODUCTION 117 4. ~ , Stotler WF. 1971. A controlled-environment culture system for high resolution light microscopy. Exp. Cell Res. 68, 144-8. 5. Jones TC, Yeh S, Hirsch JG. 1972. The interaction between Toxoplasma gondii and mammalian cells. I. Mechanism of entry and intracellular fate of the parasite. J. Exp. Med. 136, 1157-72. 6. ___ , Len L, Hirsch JG. 1975. Assessment in uitro of immunity against Toxoplasma gondii. J . Exp. Med. 141, 466-82. 7. Kaufman HE, Maloney E. 1962. Multiplication of three strains of Toxoplasma gondii in tissue culture. J. Parasitol. 48, 358-61. 9. Lycke E, Lund E. 1964. A tissue culture method for titration of infectivity and determination of growth rat? of Toxoplasma gondii. 2. Acta Pathol. Microbiol. Scan. 60, 221-33. 10. Moore GE, Gerner RE, Franklin HA. 1967. Culture of normal human leukocytes. J. Am. Med. Assoc. 199. 519-24. 11. Pfefferkorn ER, Pfefferkorn LC. 1976. Toxoplasma gondii: isolation and preliminary characterization of temperaturesensitive mutants. Exp. Parasitol. 39, 365-76. 12. Sethi KK, Pelster B, Suzuki N, Piekarski G, Brandis H 1975. Immunity to Toxoblasma eondii induced in rttro in non-8. ___ , Remington JA, .Jacobs L. 1958. Toxoplasmosis : immune mouse macrophages with specifically immune lymphvcytes. the nature of virulence. Am. J. Ophthalmol. 46, 255-60. SYNOPSISEighteen strains of algae, including 17 formerly symbiotic with Paramecium bursaria, were tested for capacity to release sugar. Detectable amounts of sugar were found in the supernatant fluids from 10 strains, includinq 6 strains infective for aposymbiotic P. bursaria syngen 2. The other 4 sugar-releasing strains were noninfective and released -26-46 pg sugar/mg dry rell weight rompared to -90-175 pg sugar/mg dry cell weight for infective strains. This relationship of infectivitv with capacity to release sugar supplements data that indicate a relationship of infectivity with resistance to Con A agglutination The correlation is completed if we assume that resistance to Con A agglutination and capacity for sugar release must both be present in a n algal strain for infectivity. The data thus strongly suggest that these 2 characteristics must be present for infectivity by any algal strains for aposymbiotic P. bursaria syngen 2.Index Key Words: Paramecium burraria syngen 2 ; symbiotic algae; infectivity; sugar release. PECIFIC sugar release from the cell is a unique feature of

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“…The same is true for infective algae the surface of which has been coated by antibodies . Other experiments showed that chlorellae which are suitable for symbiosis formation are similar in Concanavalin A agglutinability, thus differing from unsuitable algae (WEIS 1978). Obviously infective algae are recognized by the Paramecium because of a special surface structure.…”

Section: Adaptive Features Of the Symbiotic Chlorella Specmentioning

confidence: 99%

Host‐symbiont Interaction in Paramecium bursaria: Physiological and Morphological Features and their Evolutionary Significance

Reißer

1981

Berichte der Deutschen Botanischen Gesellschaft

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Correlation of Infectivity and Concanavalin A Agglutinability of Algae Exsymbiotic from Paramecium bursaria

Cited by 40 publications

References 17 publications

Viruses of symbiotic Chlorella -like algae isolated from Paramecium bursaria and Hydra viridis

Viruses of symbiotic Chlorella -like algae isolated from Paramecium bursaria and Hydra viridis

Correlation of Sugar Release and Concanavalin A Agglutinability with Infectivity of Symbiotic Algae from Paramecium bursaria for Aposymbiotic P. bursaria

Host‐symbiont Interaction in Paramecium bursaria: Physiological and Morphological Features and their Evolutionary Significance

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