Correlation of in vitro genotoxicity and oncogenicity induced by radiation and asbestos fibres

Hei, Tom K.; Geard, Charles R.; Osmak, R S; Travisano, M

doi:10.1038/bjc.1985.231

Cited by 29 publications

(6 citation statements)

References 18 publications

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“…Human mesothelial cells are exquisitely sensitive to the cytotoxic effects of asbestos (6), and asbestos causes both chromosomal aberrations and morphological transformation in human (6) and rodent (7) mesothelial cells. In fibroblasts (8) and epithelial cells (9), but not mesothelial cells (10), asbestos also enhances cell transformation by other agents such as polycyclic aromatic hydrocarbons. These results, and the lack of correlation between smoking history and the development of mesothelioma in humans (1), suggest that asbestos fibers may be complete carcinogens in the development of mesothelioma.…”

mentioning

confidence: 99%

Persistent induction of c-fos and c-jun expression by asbestos.

Heintz

Janssen

Mossman

1993

Proc. Natl. Acad. Sci. U.S.A.

188

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To investigate the mechanisms of asbestos-induced carcinogenesis, expression of c-fos and c-jun protooncogenes was examined in rat pleural mesothelial cells and hamster tracheal epithelial cells after exposure to crocidolite or chrysotile asbestos. In contrast to phorbol 12-myristate 13-acetate, which induces rapid and transient increases in c-fos and c-jun mRNA, asbestos causes 2- to 5-fold increases in c-fos and c-jun mRNA that persist for at least 24 hr in mesothelial cells. The induction of c-fos and c-jun mRNA by asbestos in mesothelial cells is dose-dependent and is most pronounced with crocidolite, the type of asbestos most pathogenic in the causation of pleural mesothelioma. Induction of c-jun gene expression by asbestos occurs in tracheal epithelial cells but is not accompanied by a corresponding induction of c-fos gene expression. In both cell types, asbestos induces increases in protein factors that bind specifically to the DNA sites that mediate gene expression by the AP-1 family of transcription factors. The persistent induction of AP-1 transcription factors by asbestos suggests a model of asbestos-induced carcinogenesis involving chronic stimulation of cell proliferation through activation of the early response gene pathway that includes c-jun and/or c-fos.

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mentioning

confidence: 99%

Persistent induction of c-fos and c-jun expression by asbestos.

Heintz

Janssen

Mossman

1993

Proc. Natl. Acad. Sci. U.S.A.

188

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“…18 This might add to the scarce preclinical evidence for the synergistic action of asbestos and radiation in the pathogenesis of mesotheliomas. 19,20 Patients treated with radiation for HL (especially in combination with chemotherapy) experience a dramatically increased risk for mesothelioma, which manifests particularly 25 years or more after treatment. Our study confirms the high risks previously reported among other groups of patients treated with high-dose radiation to the chest.…”

Section: Results and Conclusionmentioning

confidence: 99%

Malignant mesothelioma after radiation treatment for Hodgkin lymphoma

Bruin¹,

Burgers

Baas

et al. 2009

Blood

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Malignant mesothelioma is a relatively uncommon malignancy. Although the pathogenesis is primarily related to asbestos, the disease may be associated with radiation exposure. Recently, increased risks for second primary mesothelioma after radiation for lymphoma have been reported. Because these findings are based on small numbers of patients, they need to be confirmed. We examined mesothelioma risk in 2567 5-year survivors of Hodgkin lymphoma. The risk was almost 30-fold increased in Hodgkin lymphoma patients treated with irradiation compared with the general population. Although histology and survival of the mesothelioma cases were comparable with cases from the general population, asbestos exposure and the proportion of males were lower than expected. The evidence for radiotherapy as cause for mesothelioma independent of exposure to asbestos is expanding, and the diagnosis of mesothelioma should be kept in mind whenever related symptoms arise in patients who had previous irradiation.

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“…A large Table 1. Boreiko et al (1980) -, Boreiko et al (1980) Asbestos (crocidolite) Hei et al (1984Hei et al ( , 1985 DES…”

Section: Discussionmentioning

confidence: 99%

Combined Effects of Tumor Promoters and Serum on Proliferin mRNA Induction: A Biomarker Sensitive to Saccharin, 2,3,7,8-Tcdd, and Other Compounds at Minimal Concentrations Promoting C3H/10T1/2 Cell Transformation

Parfett

2003

Journal of Toxicology and Environmental Health, Part A

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Increases in proliferin (PLF) gene family mRNA abundance and promotional effects in cell transformation assays are paired responses that follow exposures to diverse chemical and physical agents in the C3H/10T1/2 in vitro model of multi-stage carcinogenesis. This study measured PLF mRNA abundance changes over 1 to 3 d in response to several types of promoters that were previously unassessed for this effect. Saccharin is a known promoter of cell transformation in C3H/10T1/2 cell cultures, but unlike 12-O-tetradecanoylphorbol 13-acetate (TPA) or mezerein, PLF mRNA abundance increases were inconsistently detected following simple addition of saccharin to the culture medium. Consistent effects occurred when pretreatments with promoting concentrations of saccharin or sodium saccharin (1-13 mM) were combined with subsequent additions of serum or complete medium changes. When added at or near their lowest observed effect levels (LOELs) for transformation, other promoters of 10T1/2 cells such as formaldehyde (50-100 microM), diethylstilbesterol (DES) (0.5-30 microM), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) (4-40 pM) were shown to be inducers of both basal and serum-induced PLF mRNA levels. Acetaldehyde (300-900 microM) was comparable to formaldehyde as an inducer. In contrast to these various promoters, pretreatment with phenobarbital or methanol, both non-promoters in these cells, did not affect serum-induced PLF mRNA levels at concentrations up to 3 mM and 2 M, respectively. The published values for the LOELs of 17 promoters of cell transformation and the LOELs determined to date for PLF mRNA induction were highly correlated over a 1 billion - fold concentration range. The response of PLF mRNA is a short-term marker sensitive to the active concentration ranges of diverse chemical agents with promotional activity in C3H/10T1/2 cell transformation system.

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Correlation of in vitro genotoxicity and oncogenicity induced by radiation and asbestos fibres

Cited by 29 publications

References 18 publications

Persistent induction of c-fos and c-jun expression by asbestos.

Persistent induction of c-fos and c-jun expression by asbestos.

Malignant mesothelioma after radiation treatment for Hodgkin lymphoma

Combined Effects of Tumor Promoters and Serum on Proliferin mRNA Induction: A Biomarker Sensitive to Saccharin, 2,3,7,8-Tcdd, and Other Compounds at Minimal Concentrations Promoting C3H/10T1/2 Cell Transformation

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