Correlation of IgA, IgM and IgG antibody-detecting assays based on filamentous haemagglutinin, pertussis toxin and <i>Bordetella pertussis</i> sonicate in a strictly adult population

Klement, Eyal; Kagan, Nikki; Hagain, L.; Kayouf, Raid; Boxman, Jonathan; Cohen, Dani; Orr, Nadav

doi:10.1017/s0950268804003206

Cited by 8 publications

(2 citation statements)

References 37 publications

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“…Anti‐PT IgG antibodies respond slower in B. pertussis infection, and the antibody is not raised fully in patients during the acute phase of the disease . A previous report described that the measurement of anti‐PT IgG antibodies remains effective for serum samples taken several weeks after the onset of the disease (coughing) . Additionally, it was shown that the anti‐PT IgG ELISA has high diagnostic accuracy (AUC, 0.96) for convalescent serum samples .…”

Section: Discussionmentioning

confidence: 99%

A Novel IgM‐capture enzyme‐linked immunosorbent assay using recombinant Vag8 fusion protein for the accurate and early diagnosis of Bordetella pertussis infection

Otsuka

Gotoh

Nishimura

et al. 2016

Microbiology and Immunology

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An ELISA that measures anti-PT IgG antibody has been used widely for the serodiagnosis of pertussis; however, the IgG-based ELISA is inadequate for patients during the acute phase of the disease because of the slow response of anti-PT IgG antibodies. To solve this problem, we developed a novel IgM-capture ELISA that measures serum anti-Bordetella pertussis Vag8 IgM levels for the accurate and early diagnosis of pertussis. First, we confirmed that Vag8 was highly expressed in all B. pertussis isolates tested (n = 30), but little or none in other Bordetella species, and that DTaP vaccines did not induce anti-Vag8 IgG antibodies in mice (i.e. the antibody level could be unaffected by the vaccination). To determine the immune response to Vag8 in B. pertussis infection, anti-Vag8 IgM levels were compared between 38 patients (acute phase of pertussis) and 29 healthy individuals using the anti-Vag8 IgM-capture ELISA. The results revealed that the anti-Vag8 IgM levels were significantly higher in the patients compared with the healthy individuals (P < 0.001). ROC analysis also showed that the anti-Vag8 IgM-capture ELISA has higher diagnostic accuracy (AUC, 0.92) than a commercial anti-PT IgG ELISA kit. Moreover, it was shown that anti-Vag8 IgM antibodies were induced earlier than anti-PT IgG antibodies on sequential patients' sera. These data indicate that our novel anti-Vag8 IgM-capture ELISA is a potentially useful tool for making the accurate and early diagnosis of B. pertussis infection.

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Section: Discussionmentioning

confidence: 99%

A Novel IgM‐capture enzyme‐linked immunosorbent assay using recombinant Vag8 fusion protein for the accurate and early diagnosis of Bordetella pertussis infection

Otsuka

Gotoh

Nishimura

et al. 2016

Microbiology and Immunology

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“…The first was a 15-month (November 2001–March 2003), laboratory-based surveillance study of pertussis, which included 110 trainees who complained of persistent coughing (case definition: cough lasting 10–90 days) upon admission to compound clinics. Samples obtained from these patients were tested by a commercial enzyme-linked immunosorbent assay (ELISA) (PanBio, East Brisbane, Queensland, Australia) for the presence of immunoglobulin (Ig) A against a Bordetella pertussis sonicate and by an in-house IgA-detection ELISA directed against pertussis toxin as previously described ( 9 ). Results for IgA to B. pertussis sonicate were calculated as arbitrary ELISA units obtained according to the manufacturer's instructions.…”

mentioning

confidence: 99%