Correlation of Deoxynivalenol Accumulation in Fusarium-Infected Winter and Spring Wheat Cultivars with Secondary Metabolites at Different Growth Stages

Etzerodt, Thomas; Gislum, René; Laursen, Britt Elmedal; Heinrichson, Kirsten; Gregersen, Per L.; Jørgensen, Lise Nistrup; Fomsgaard, Inge S.

doi:10.1021/acs.jafc.6b01162

Cited by 23 publications

(27 citation statements)

References 43 publications

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“…Grains from the selected spikes were used for analysis of mycotoxins and HvNEP-1 expression. Mycotoxin accumulation (DON, NIV, ZEA, T-2, and HT-2) in the seeds of the transgenic lines and control GP plants was quantified in an LC-MS/MS system consisting of an Agilent 1260 infinity chromatographic system (Santa Clara, CA, USA) coupled to an AB Sciex 3200 triple-quadrupole trap mass spectrometer (QTRAP/MS) (AB Sciex, Framingham, USA) according to Etzerodt et al [46]. Analyst 1.6.3 software (AB Sciex, Framingham, USA) was used to control the LC-MS/MS system.…”

Section: Disease Scoring and Mycotoxin Analysismentioning

confidence: 99%

Overexpression of Nepenthesin HvNEP-1 in Barley Endosperm Reduces Fusarium Head Blight and Mycotoxin Accumulation

et al. 2020

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Fusarium head blight (FHB) causes substantial losses of yield and quality in grains, both in the field and in post-harvest storage. To date, adequate natural genetic resistance is not available for the control of FHB. This study reports the cloning and overexpression of a barley (Hordeum vulgare L.) antifungal gene, nepenthesin 1 (HvNEP-1), in the endosperm of barley grains. Transgenic barley lines overexpressing HvNEP-1 substantially reduced FHB severity and disease progression after inoculation with Fusarium graminearum or Fusarium culmorum. The transgenic barley also showed reduced accumulation of the mycotoxin deoxynivalenol (DON) in grain, far below the minimum value allowable for food. Semi-field evaluation of four HvNEP-1 transgenic lines revealed substantial reduction of FHB severity and progression as compared with the control H. vulgare cultivar Golden promise (GP) plants. Our study demonstrated the utility of HvNEP-1 for the control of FHB in barley, and possibly other grains such as wheat and maize.

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Section: Disease Scoring and Mycotoxin Analysismentioning

confidence: 99%

Overexpression of Nepenthesin HvNEP-1 in Barley Endosperm Reduces Fusarium Head Blight and Mycotoxin Accumulation

et al. 2020

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“…In previous studies, it has been found that these strains are common in Denmark [ 76 ]. Spore suspensions of both strains were prepared and sprayed according to Etzerodt et al [ 77 ]. Briefly, spikes were sprayed directly with a Fusarium spp.…”

Section: Materials Methodsmentioning

confidence: 99%

“…A visual linear scale from 0 to 10 was used to assess the infected plants within each plot. The fusariotoxin accumulations (DON, NIV, and ZEN) in the seeds of inoculated hybrids were quantified in an LC-MS/MS system consisting of an Agilent 1260 infinity chromatographic system (Santa Clara, CA, USA) coupled to an AB Sciex 3200 triple quadrupole trap mass spectrometer (QTRAP/MS) (AB Sciex, Framingham, MA, USA), as described by Etzerodt et al [ 77 ]. Analyst 1.6.3 software (AB Sciex, Woodlands Central Indus.…”

Section: Materials Methodsmentioning

confidence: 99%

A Comparative Transcriptome Analysis, Conserved Regulatory Elements and Associated Transcription Factors Related to Accumulation of Fusariotoxins in Grain of Rye (Secale cereale L.) Hybrids

Mahmood

Orabi²,

Kristensen³

et al. 2020

IJMS

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Detoxification of fusariotoxin is a type V Fusarium head blight (FHB) resistance and is considered a component of type II resistance, which is related to the spread of infection within spikes. Understanding this type of resistance is vital for FHB resistance, but to date, nothing is known about candidate genes that confer this resistance in rye due to scarce genomic resources. In this study, we generated a transcriptomic resource. The molecular response was mined through a comprehensive transcriptomic analysis of two rye hybrids differing in the build-up of fusariotoxin contents in grain upon pathogen infection. Gene mining identified candidate genes and pathways contributing to the detoxification of fusariotoxins in rye. Moreover, we found cis regulatory elements in the promoters of identified genes and linked them to transcription factors. In the fusariotoxin analysis, we found that grain from the Nordic seed rye hybrid “Helltop” accumulated 4 times higher concentrations of deoxynivalenol (DON), 9 times higher nivalenol (NIV), and 28 times higher of zearalenone (ZEN) than that of the hybrid “DH372” after artificial inoculation under field conditions. In the transcriptome analysis, we identified 6675 and 5151 differentially expressed genes (DEGs) in DH372 and Helltop, respectively, compared to non-inoculated control plants. A Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that DEGs were associated with glycolysis and the mechanistic target of rapamycin (mTOR) signaling pathway in Helltop, whereas carbon fixation in photosynthesis organisms were represented in DH372. The gene ontology (GO) enrichment and gene set enrichment analysis (GSEA) of DEGs lead to identification of the metabolic and biosynthetic processes of peptides and amides in DH372, whereas photosynthesis, negative regulation of catalytic activity, and protein-chromophore linkage were the significant pathways in Helltop. In the process of gene mining, we found four genes that were known to be involved in FHB resistance in wheat and that were differentially expressed after infection only in DH372 but not in Helltop. Based on our results, we assume that DH372 employed a specific response to pathogen infection that led to detoxification of fusariotoxin and prevented their accumulation in grain. Our results indicate that DH372 might resist the accumulation of fusariotoxin through activation of the glycolysis and drug metabolism via cytochrome P450. The identified genes in DH372 might be regulated by the WRKY family transcription factors as associated cis regulatory elements found in the in silico analysis. The results of this study will help rye breeders to develop strategies against type V FHB.

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“…In a research using wheat, principal component analyses demonstrated a correlation between the susceptibility to FHB and the concentrations of range of Bxs [57]. The benzoxazinoid 2-β-glucopyranoside-2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3one (DIMBOA-glc), α-tocopherol and the flavonoids homo-orientin and orientin were identified as potential inhibitors of (deoxynivalenol) DON accumulation in a study with wheat that correlates accumulation in Fusarium-infected winter and spring wheat cultivars [58].…”

Section: Benzoxazinoidmentioning

confidence: 96%

Fusarium Mycotoxins and Metabolites that Modulate Their Production

Jiménez-García¹,

García-Mier²,

García‐Trejo³

et al. 2018

Fusarium - Plant Diseases, Pathogen Diversity, Genetic Diversity, Resistance and Molecular Markers

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The genus Fusarium is a group of fungi producing several types of toxins with toxicological effect in both humans and animals. Such fungi are commonly found in soils so it can contaminate various types of crops, preferably cereals, leading to significant economic losses. Relative humidity, storage temperature and various handling in cereales increase the possibility of contamination by Fusarium toxins. Cereals naturally have secondary metabolites that may help attenuate contamination by these toxins, but it is necessary to know strategies and mechanisms that generate inactivation mycotoxins. This chapter reviews relevant information about cereal mycotoxin contamination, as well as the production of cereal secondary metabolites as a strategy to reduce the possibility of mycotoxin contamination.

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Correlation of Deoxynivalenol Accumulation in Fusarium-Infected Winter and Spring Wheat Cultivars with Secondary Metabolites at Different Growth Stages

Cited by 23 publications

References 43 publications

Overexpression of Nepenthesin HvNEP-1 in Barley Endosperm Reduces Fusarium Head Blight and Mycotoxin Accumulation

Overexpression of Nepenthesin HvNEP-1 in Barley Endosperm Reduces Fusarium Head Blight and Mycotoxin Accumulation

A Comparative Transcriptome Analysis, Conserved Regulatory Elements and Associated Transcription Factors Related to Accumulation of Fusariotoxins in Grain of Rye (Secale cereale L.) Hybrids

Fusarium Mycotoxins and Metabolites that Modulate Their Production

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