Correlation of autoagglutination and virulence of yersiniae

Laird, W; Cavanaugh, Dan C.

doi:10.1128/jcm.11.4.430-432.1980

Cited by 275 publications

(144 citation statements)

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“…The identification of yersiniae was performed by the methods of Wauters et al (1988). All isolates identified as yersiniae were subjected to autoagglutination tests to evaluate their potential pathogenicity (Laird and Cavanaugh, 1980). Then, virulent isolates were subjected to further analysis.…”

Section: Isolation and Identification Of Yersiniamentioning

confidence: 99%

Prevalence of Salmonella, Yersinia and Campylobacter spp. in Feral Raccoons (Procyon lotor) and Masked Palm Civets (Paguma larvata) in Japan

Lee

Iwata

Nakadai

et al. 2011

Zoonoses and Public Health

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To estimate the public and animal health risk that alien species pose, the prevalence of Salmonella, Yersinia, and Campylobacter spp. in feral raccoons (Procyon lotor, n=459) and masked palm civets (Paguma larvata, n=153), which are abundant alien species in Japan, was investigated in urban and suburban areas of Japan. Salmonella enterica was detected from 29 samples [26 raccoons, 5.7%, 95% confidence interval (CI) 7.8-3.5%; three masked palm civets, 2.0%, 95% CI 4.2-0%]. Many of the isolates belonged to serovars that are commonly isolated from human gastroenteritis patients (e.g. S. Infantis, S. Typhimurium, and S. Thompson). The antimicrobial susceptibility test showed that 26.9 % of the isolates from raccoons were resistant to at least one antimicrobial agent, whereas none of the isolates from masked palm civets were resistant. Yersinia sp. was detected from 193 samples (177 raccoons, 38.6%, 95% CI 43.0-34.1%; 16 masked palm civets, 10.5%, 95% CI 15.3-5.6%). All virulent Yersinia strains belonged to Yersinia pseudotuberculosis, which was isolated from seven (1.5%, 95% CI 2.6-0.4%) raccoons and six (3.9%, 95% CI 7.0-0.8%) masked palm civets. According to the detection of virulence factors, all the Y. pseudotuberculosis isolates belonged to the Far Eastern systemic pathogenicity type. Campylobacter spp. was detected from 17 samples (six raccoons, 1.3%, 95% CI 2.3-0.3%; 11 masked palm civets, 7.2%, 95% CI 11.3-3.1%). Among these, three isolates from raccoons were identified as C. jejuni. These results showed that these pathogens can be transmitted by human activities, other wild animals, and the environment to feral raccoons and masked palm civets, and vice versa. As these animals have omnivorous behaviour and a wide range of habitats, they can play an important role in the transmission of the enteric pathogens.

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Section: Isolation and Identification Of Yersiniamentioning

confidence: 99%

Prevalence of Salmonella, Yersinia and Campylobacter spp. in Feral Raccoons (Procyon lotor) and Masked Palm Civets (Paguma larvata) in Japan

Lee

Iwata

Nakadai

et al. 2011

Zoonoses and Public Health

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“…A number of studies (McDermott et al 1993;Goverde et al 1994) have shown that the choice of incubation temperature affects the growth kinetics of plasmid-bearing and plasmid-cured strains of Escherichia coli and Y. enterocolitica. In general, virulence plasmid expression in Yersinia occurs at temperatures > 30 C, this expression is usually observed in phenotypic characteristics such as calcium dependency, autoagglutination and production of outer membrane proteins (Laird and Cavanaugh 1980;Cornelis et al 1987;Bhaduri et al 1990). Goverde et al (1994) reported that virulence plasmid expression at temperatures greater than 30 C retards the growth rate of plasmid-bearing Y. enterocolitica strains as plasmid replication places an additional metabolic burden on growing cells.…”

Section: Introductionmentioning

confidence: 99%

The effect of temperature and selective agents on the growth of Yersinia enterocolitica serotype O:3 in pure culture

et al. 2000

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This study examined the individual and combined effects of the selective agents normally present in Yersinia-selective agar (i.e. cefsulodin, irgasan and novobiocin) on the growth kinetics of plasmid-bearing (P) and plasmid-cured (P±) Yersinia enterocolitica serotype O:3 at 25 and 37 C. Growth studies were carried out in pure culture, and the data obtained were subjected to linear regression analysis to determine lag phase duration(s) and growth rates of the examined strains. In general, the presence of selective agents increased the duration of the lag phase at 37 C, with longer lag phases noted in all cases in which two or more selective agents were present. Growth rates in CIN broth base (CIN NA) and CIN NA plus commercial supplement (SR 109) (CIN) were faster at 37 than 25 C, but in some cultures of incomplete CIN NA broth with less than three supplements added, growth tended to be faster at 25 than 37 C. Generally, plasmid-bearing strains grew slower than plasmid-cured strains in most media at 37 C due to virulence plasmid expression retarding growth. In some instances at 37 C, it was observed that the growth rates of both plasmid-bearing and plasmid-cured strains were comparable, indicating the in¯uence of added selective agent/s negating any effects associated with virulence plasmid expression. The effects of selective agents, incubation temperature and virulence plasmid carriage on the growth kinetics of Y. enterocolitica are discussed.

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“…The temperature outside the host fluctuates and is usually tess than 2,7''C, while the environment Inside the host is thermally regulated at 37°C; consequently, several Y. enteroootitioa virulence genes are regulated by temperature and other host stimuli to facilitate the hostpathogen interaction. Pathogenic Yersinia species harbour a related 70 kb virulence piasmid that encodes many thermally regulated virulence phenotypes such as autoagglutinability (Laird and Cavanaugh, 1980), cytotoxicity (Portnoy et ai, 1981), and serum resistance (Pai and DeStephano, 1982). The invasive phenotype of Y. enterocoiitica is only associated with pathogenic strains and is regulated by growth temperature (Lee et al, 1977;Miller etai, 1989;Pierson and Falkow, 1990;Schiemann and Devenish, 1982).…”

Section: Introductionmentioning

confidence: 99%

Growth phase and low pH affect the thermal regulation of the Yersinia enterocolitica inv gene

Pepe

Badger

Miller

1994

Molecular Microbiology

146

160

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The inv gene encodes the protein invasin, which is the primary invasion factor for Yersinia enterocolitica in vitro and in vivo. Previous studies of Yersinia species have shown that inv expression and entry into mammalian cells are temperature regulated. Invasin production is reduced at the host temperature of 37 degrees C as compared to production at ambient temperature; consequently, this study was initiated to determine whether other host environmental signals might induce inv expression at 37 degrees C. An inv::phoA translational fusion was recombined on to the Y. enterocolitica chromosome by allelic exchange to monitor inv expression. Molecular characterization of expression of the wild-type inv gene and the inv::phoA fusion showed that invasin is not produced until early stationary phase in bacteria grown at 23 degrees C. Y. enterocolitica grown at 37 degrees C and pH 5.5 showed levels of inv expression comparable to those observed in bacteria grown at 23 degrees C. An increase in Na+ ions caused a slight increase in expression at 37 degrees C. However, expression at 37 degrees C was unaffected by anaerobiosis, growth medium, calcium levels, or iron levels. Additionally, Y. enterocolitica expressed invasin in Peyer's patches two days after being introduced intragastrically into BALB/c mice. These results suggest that invasin expression in Y. enterocolitica may remain elevated early during interaction with the intestinal epithelium, a site at which invasin was shown to be necessary.

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Correlation of autoagglutination and virulence of yersiniae

Cited by 275 publications

References 1 publication

Prevalence of Salmonella, Yersinia and Campylobacter spp. in Feral Raccoons (Procyon lotor) and Masked Palm Civets (Paguma larvata) in Japan

Prevalence of Salmonella, Yersinia and Campylobacter spp. in Feral Raccoons (Procyon lotor) and Masked Palm Civets (Paguma larvata) in Japan

The effect of temperature and selective agents on the growth of Yersinia enterocolitica serotype O:3 in pure culture

Growth phase and low pH affect the thermal regulation of the Yersinia enterocolitica inv gene

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