Correlation between variable-number tandem-repeat-based genotypes and drug susceptibility in Mycobacterium avium isolates

Tatano, Yutaka; Sano, Chiaki; Yasumoto, Ko; Shimizu, Toshiaki; Sato, Katsumasa; Nishimori, Kei; Matsumoto, Tomoshige; Yano, Shuichi; Takeyama, Hiroyasu; Tomioka, Haruaki

doi:10.1007/s10096-011-1326-7

Cited by 7 publications

(5 citation statements)

References 33 publications

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“…However, 3 strains, DH-2, IH-068, and NN-127, were grouped into the clusters containing strains isolated abroad, suggesting that the strains in cluster I are genetically similar to those isolated abroad. Previous studies reported the association between genotype and drug susceptibility in NTM (29,30). In the present study, significant differences in the log 2 MICs of seven of eight of the drugs tested, with the exception being ethambutol, were observed among the isolates in the three clusters grouped by VNTR typing of M. avium subsp.…”

Section: Discussionsupporting

confidence: 56%

Antibiotic Susceptibility and Genotyping of Mycobacterium avium Strains That Cause Pulmonary and Disseminated Infection

Uchiya

Asahi

Futamura

et al. 2018

Antimicrob Agents Chemother

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subsp. mainly causes disseminated infection in immunocompromised hosts, such as individuals with human immunodeficiency virus (HIV) infection, and pulmonary infection in immunocompetent hosts. However, many aspects of the different types of subsp. infection remain unclear. We examined the antibiotic susceptibilities and genotypes of subsp. isolates from different hosts by performing drug susceptibility testing using eight antibiotics (clarithromycin, rifampin, ethambutol, streptomycin, kanamycin, amikacin, ethionamide, and levofloxacin) and variable-number tandem-repeat (VNTR) typing analysis for 46 isolates from the sputa of HIV-negative patients with pulmonary subsp. disease without previous antibiotic treatment and 30 isolates from the blood of HIV-positive patients with disseminated subsp. disease. Interestingly, isolates from pulmonary subsp. disease patients were more resistant to seven of the eight drugs, with the exception being rifampin, than isolates from HIV-positive patients. Moreover, VNTR typing analysis showed that the strains examined in this study were roughly classified into three clusters, and the genetic distance from reference strain 104 for isolates from pulmonary subsp. disease patients was statistically significantly different from that for isolates from HIV-positive patients ( = 0.0018), suggesting that subsp. strains that cause pulmonary and disseminated disease have genetically distinct features. Significant differences in susceptibility to seven of the eight drugs, with the exception being ethambutol, were noted among the three clusters. Collectively, these results suggest that an association between the type of subsp. infection, drug susceptibility, and the VNTR genotype and the properties of subsp. strains associated with the development of pulmonary disease are involved in higher levels of antibiotic resistance.

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Section: Discussionsupporting

confidence: 56%

Antibiotic Susceptibility and Genotyping of Mycobacterium avium Strains That Cause Pulmonary and Disseminated Infection

Uchiya

Asahi

Futamura

et al. 2018

Antimicrob Agents Chemother

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“…No association of a specific VNTR pattern with a particular clinical feature, such as the pulmonary or lymph node infection, was observed. Further studies will provide information about possible association between MAH genotypes and other clinical characteristics in our geographical region; about this, other studies report that in Japan M. avium genotypes were associated with disease progression or therapeutic response (Kikuchi et al, 2009;Kikuchi et al, 2014), or that there was no significant association between MAH genotypes isolated from Korean patients and clinical manifestation or treatment response (Kim et al, 2016;Kim et al, 2012;Tatano et al, 2012).…”

Section: Association Between Vntr Genotypes and Characteristics Of Ma...mentioning

confidence: 92%

“…The tree was generated using the UPGMA method by the MIRU-VNTRplus web application available at www.miru-vntrplus.org. 2018) and a further study has reported a significant association between VNTR genotypes and susceptibility to quinolones (Tatano et al, 2012).…”

Section: Association Between Vntr Genotypes and Characteristics Of Ma...mentioning

confidence: 94%

Genotyping and clarithromycin susceptibility testing of Mycobacterium avium subsp. hominissuis isolated in Tuscany, Italy

Genua

Menichini

Lari

et al. 2018

Infection, Genetics and Evolution

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“…Interestingly, the presence of IS Mav6 was associated with drug resistance to MXF in this study. Tatano et al reported an association between the VNTR genotype and susceptibility to quinolones and EMB [ 40 ]. Dvorska et al found no relationship between IS 1311 and IS 1245 -based RFLP genotypes and drug susceptibility in MAC isolates [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Association of ISMav6 with the Pattern of Antibiotic Resistance in Korean Mycobacterium avium Clinical Isolates but No Relevance between Their Genotypes and Clinical Features

et al. 2016

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The aim of this study was to genetically characterize clinical isolates from patients diagnosed with Mycobacterium avium lung disease and to investigate the clinical significance. Multi-locus sequencing analysis (MLSA) and pattern of insertion sequence analysis of M. avium isolates from 92 Korean patients revealed that all isolates were M. avium subspecies hominissuis. In hsp65 sequevar analysis, codes 2, 15, and 16 were most frequently found (88/92) with similar proportions among cases additionally two isolates belonging to code N2 and an unreported code were identified, respectively. In insertion element analysis, all isolates were IS1311 positive and IS900 negative. Four of the M. avium subsp. hominissuis isolates did not harbor IS1245 and 1 of the M. avium isolates intriguingly harbored DT1, which is thought to be a M. intracellulare-specific element. M. avium subsp. hominissuis harboring ISMav6 is prevalent in Korea. No significant association between clinical manifestation and treatment response has been found in patients with the hsp65 code type and ISMav6, indicating that no specific strain/genotype among M. avium subsp. hominissuis organisms was a major source of M. avium lung disease. Interestingly, the presence of ISMav6 was correlated with greater resistance to moxifloxacin. Conclusively, the genotype of Korean M. avium subsp. hominissuis isolates is not a disease determinant responsible for lung disease and specific virulent factors of M. avium subsp. hominissuis need to be investigated further.

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Correlation between variable-number tandem-repeat-based genotypes and drug susceptibility in Mycobacterium avium isolates

Cited by 7 publications

References 33 publications

Antibiotic Susceptibility and Genotyping of Mycobacterium avium Strains That Cause Pulmonary and Disseminated Infection

Antibiotic Susceptibility and Genotyping of Mycobacterium avium Strains That Cause Pulmonary and Disseminated Infection

Genotyping and clarithromycin susceptibility testing of Mycobacterium avium subsp. hominissuis isolated in Tuscany, Italy

Association of ISMav6 with the Pattern of Antibiotic Resistance in Korean Mycobacterium avium Clinical Isolates but No Relevance between Their Genotypes and Clinical Features

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