Genotyping and clarithromycin susceptibility testing of Mycobacterium avium subsp. hominissuis isolated in Tuscany, Italy

Genua, Flavia; Menichini, M.; Lari, Nicoletta; Rindi, Laura

doi:10.1016/j.meegid.2018.07.032

Cited by 4 publications

(5 citation statements)

References 37 publications

(56 reference statements)

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“…These data differs to what has recently been demonstrated for M. avium subsp. hominissuis strains, isolated in the same geographical area, that displayed a close genetic relationship [ 15 ], and therefore support the hypothesis that M. intracellulare and M. avium may have different sources of infection and transmission pathways, as suggested in a recent studies [ 6 , 14 ]. However, this study has some limitations: the population size was small and phenotypic features such as drug susceptibility and clinical characteristics of the strains were not investigated.…”

Section: Resultssupporting

confidence: 71%

High genetic heterogeneity of Mycobacterium intracellulare isolated from respiratory specimens

Lari

Rindi

2022

BMC Microbiol

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Background M. intracellulare is a frequent causative pathogen of nontuberculous mycobacteria infection that causes infections in the respiratory tract, whose incidence is increasing in many countries. This study aimed at determining the VNTR-based genetic diversity of a collection of 39 M. intracellulare human strains isolated from respiratory specimens over the last 5 years. Results The VNTR analysis showed that M. intracellulare strains displayed a high genetic diversity, indicating that the M. intracellulare genotypes are quite heterogeneous in our geographical area. Moreover, a comparison with VNTR profiles of strains from other countries confirmed that genotypes of clinical strains of M. intracellulare are not related to geographical origin. Conclusions VNTR typing has proved to be a highly discriminatory method for better understanding the molecular epidemiology of M. intracellulare.

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Section: Resultssupporting

confidence: 71%

High genetic heterogeneity of Mycobacterium intracellulare isolated from respiratory specimens

Lari

Rindi

2022

BMC Microbiol

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“…hominissuis isolates originating from birds and, of these, even fewer that have been genotyped by MIRU-VNTR. The MIRU-VNTR profile new 1, detected here in a Red-crowned parakeet isolate, was identified before in two human isolates from Italy ( 39 ). Another profile, INMV51, was uniquely detected in an isolate of a Red-crested turaco kept in a German zoo ( 32 ) but was also determined in isolates from different mammals in Germany (Möbius, unpublished results) and in human, porcine, or bovine isolates in different European countries and Japan ( 22 , 38 – 41 ).…”

Section: Discussionsupporting

confidence: 54%

“…avium profile was suspected in the mixed isolate from Tambourine dove together with subsp. hominissuis profile INMV93, which was identified before in clinical isolates from 15 patients from Italy ( 39 ).…”

Section: Discussionmentioning

confidence: 76%

“…Another profile, INMV51, was uniquely detected in an isolate of a Red-crested turaco kept in a German zoo ( 32 ) but was also determined in isolates from different mammals in Germany (Möbius, unpublished results) and in human, porcine, or bovine isolates in different European countries and Japan ( 22 , 38 – 41 ). However, these MIRU-VNTR results give only some hints concerning the genetic similarity of the isolates, while differences in other genomic sequence regions are still possible as shown here using combined genotyping and in various studies worldwide ( 37 , 38 , 42 ), which may even be associated with phenotypic differences ( 39 , 40 ).…”

Section: Discussionmentioning

confidence: 98%

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Mycobacteriosis in Various Pet and Wild Birds from Germany: Pathological Findings, Coinfections, and Characterization of Causative Mycobacteria

2022

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In this study, the causative agents and confounding factors of mycobacteriosis in a set of pet and some wild birds from Germany were examined. Not only Mycobacterium genavense , but also M. avium subsp. avium and M. avium subsp. hominissuis , contributed to mycobacteriosis in these birds.

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“…Pigs are thought to be a potential source of exposure for human MAH infection (14, 15). Recently, several molecular epidemiological studies of MAH involving VNTR analysis have been described from around the world (6, 16, 17, 18, 19, 20). Some reports showed epidemiological relationships of MAH strains between humans and pigs (6, 20, 21, 22).…”

Section: Introductionmentioning

confidence: 99%

Unique genomic sequences in a novelMycobacterium aviumsubsp.hominissuislineage enable fine scale transmission route tracing during pig movement

Komatsu

Ohya

Ota

et al. 2022

Preprint

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Mycobacterium avium subsp. hominissuis (MAH) is one of the most prevalent mycobacteria causing non-tuberculous mycobacterial (NTM) disease in human and animals. Of note, MAH is a major cause of mycobacterial granulomatous mesenteric lymphadenitis outbreaks in pig populations. To determine the precise source of infection of MAH in a pig farm and to clarify the epidemiological relationship among pig and human MAH lineages, we collected 50 MAH isolates from pigs reared in Japan and determined draft genome sequences of 30 isolates. We conducted a variable number of tandem repeat (VNTR), genome phylogenetic tree analysis, historical recombination analysis and genome content analysis. Consistent with the results of previous studies, VNTR revealed that most pig MAH isolates in Japan were closely related to North American, European and Russian human isolates but not to those from East Asian human. Recombination analysis indicated that most pig isolates could be classified into SC2/4 and SC3. To our surprise, four isolates belonged to a new lineage in the global MAH population. Members of this group had few footprints of inter-lineage recombination in the genome, and carried 80 unique genes, most of which were located on lineage specific-genomic islands. As the members of this new lineage were rare, we were able to trace the putative transmission route via their host pigs. Together, these results reveal the diversity in the pig MAH population and clarify the difference of source of infection and the vector of transmission between humans and pigs.

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Genotyping and clarithromycin susceptibility testing of Mycobacterium avium subsp. hominissuis isolated in Tuscany, Italy

Cited by 4 publications

References 37 publications

High genetic heterogeneity of Mycobacterium intracellulare isolated from respiratory specimens

High genetic heterogeneity of Mycobacterium intracellulare isolated from respiratory specimens

Mycobacteriosis in Various Pet and Wild Birds from Germany: Pathological Findings, Coinfections, and Characterization of Causative Mycobacteria

Unique genomic sequences in a novelMycobacterium aviumsubsp.hominissuislineage enable fine scale transmission route tracing during pig movement

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