Correlation between the synthetic activity of lymphocytes and the intensity of neutron radiation near the Earth surface

One of the important methods to study the intracellular regulation of metabolism is the fluorescent spectroscopy. Fluorochrome acridine orange (AO) is widely used to investigate the nucleic acids in living as well as fixed cells. The objective of this research was to study the functional activity of the cell synthesizing apparatus with spectral fluorescent method. The cell functional (synthetic) activity is described by the parameter α representing the RNA/DNA ratio in fixed cells stained with AO. The possibilities of fluorescent cytodiagnosis have been studied to analyze the blood system cells in some diseases, under ionizing and electromagnetic irradiations, in natural and artificial hypomethabolic states as well as nervous and reproductive systems in the mollusks.

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“…This system was described in detail elsewhere [1,5]. It was based on a fluorescent microscope with a double-channel fluorescent sensor assembly.…”

Section: Methodsmentioning

confidence: 99%

“…The objective of this research was to study the functional activity of the cell synthesizing apparatus with spectral fluorescent method using microfluorimeter DMF-2 (Radical, Russia) [5]. The cell functional (synthetic) activity is described by the parameter α representing the RNA/DNA ratio in fixed cells stained with AO.…”

Section: Introductionmentioning

confidence: 99%

<title>Fluorescent analysis in cell monitoring of environment and human health</title>

2008

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“…The size of the photometered area corresponds to the cell size. A special program "Microfluor" made it possible to obtain the distribution histograms of the fluorescence intensities in the different regions of the spectrum as well as the distribution histograms of the characteristic parameters for 200 cells within 15-20 min with the points plotted onto the phase plane, and to perform statistical analysis of the data [4,5]. These techniques are useful especially in medical and ecological investigations.…”

Section: Microfluorimeter Dmf-2mentioning

confidence: 99%

Application of microspectral luminescent analysis to study the intracellular metabolism in single cells and cell systems

Karnaukhova¹,

Sergievich²,

Karnaukhov³

2010

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Spectral luminescent analysis of single cells and cellular systems enable us to reveal the initial changes of intracellular metabolism that can followed by human diseases or failure in biocenosis. Two cytodiagnostic systems of de-vices and techniques have been developed: 1) Microspectrofluorimeters registering the fluo-rescent spectra of individual cells or intracellular organelles used for fundamental investigations of cell reactions and for discovering and studying new dimensionless fluorescent characteristic parameters reflecting the biochemical or physiological properties of the cells; 2) Double- and multi-wave microfluorimeters for rapid registration of fluorescent characteristic para- meters for many cells to obtain statistical information about cell population. These techni- ques are useful especially in medical and eco-logical investigations

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Correlation between the synthetic activity of lymphocytes and the intensity of neutron radiation near the Earth surface

Cited by 2 publications

References 2 publications

<title>Fluorescent analysis in cell monitoring of environment and human health</title>

<title>Fluorescent analysis in cell monitoring of environment and human health</title>

Application of microspectral luminescent analysis to study the intracellular metabolism in single cells and cell systems

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