Application of microspectral luminescent analysis to study the intracellular metabolism in single cells and cell systems

Karnaukhova, N. A.; Sergievich, L. A.; Karnaukhov, V. N.

doi:10.4236/ns.2010.25054

Cited by 5 publications

(3 citation statements)

References 6 publications

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“…Если соотношение FAD/NADH увеличивается, то состояние митохондрии расценивается как активное. Это сопровождается высо-ким содержанием АДФ, высоким содержанием субстрата и интенсивным дыханием [3], что в целом свидетельствует о неполном восстановлении организма спортсменов КГ после физической нагрузки.…”

Section: спортивное питаниеunclassified

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Effect of a Whey-Based Bioproduct on Metabolic Processes in the Microcirculation

Bruk

Литвин

Terekhov

2019

hsm

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Aim. The article deals with the effect of a whey-based product on the metabolic processes, character, and local mechanisms of the microcirculation in athletes involved in track-and-field athletics. Materials and methods. 32 male athletes participated in the study. The microcirculation was studied with the help of the LAKK-M laser analyzer. The calculations were conducted by using a special software package (version 2.0.0.423, LAZMA, Russia). Results. It is established that a course of the MDX Multicomplex bioproduct increases blood perfusion in the microcirculatory bloodstream by 34% and erythrocyte fluctuation by 66%, which improve the efficiency of metabolic processes. Bloodstream modulation is provided by the enhanced performance of the local active regulatory mechanisms. A statistically significant increase of 42% is registered in the endothelium-dependent mechanism, the increase of 32% and 23% is found in the myogenic and neurogenic mechanisms respectively. The contribution of the passive regulatory mechanisms reduces as a result of chest and heart muscle performance. The physiologically active substances of the bioproduct are involved in the vasodilation of microvessels and redox reactions at the cellular level. A 22% increase in NADH reduced coenzyme and a 9% decrease in FAD oxidized coenzyme indicate a decrease in mitochondrial activity in the absence of physical exertion and, indirectly, confirm the complete restoration of the energy reserves. Conclusion. The data obtained proves the optimization of metabolic processes in the microcirculation as an effect of the MDX Multicomplex bioproduct.

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Section: спортивное питаниеunclassified

“…Как отмечает В.Н. Карнаухов [3], это отношение является важной частью окислительновосстановительного состояния клетки -мерой ее метаболической активности -и уровня жизнедеятельности клетки.…”

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Effect of a Whey-Based Bioproduct on Metabolic Processes in the Microcirculation

Bruk

Литвин

Terekhov

2019

hsm

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“…The modern ecological investigations require reliable express-methods for the estimation of a functional state of living organisms. The luminescent method based on the analysis of red photoluminescence, whose centers are chlorophyll a molecules [1], has been successfully used in the plant diagnostics for a long time.…”

Section: Introductionmentioning

confidence: 99%

Degradation of Chlorophyll Luminescence in Plants

2012

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The chlorophyll photoluminescence intensity degradation of Vallisneria spiralis L. water plant is studied. It is shown that the degradation curve is rather well described by a sum of two hyperbolic functions. The rate of intensity degradation reduces at low temperatures. At room temperature, a slow restoration of the luminescent system is observed after the irradiation has been ceased. No restoration is detected at the liquid nitrogen temperature. A simplified model which describes the luminescence degradation according to the quadratic law is suggested.

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Microspectral method for assessing the functional state of chickens’ hepatocytes under intestinal infectious diseases

Akchurin

Ларионов

Красникова

et al. 2021

J. Phys.: Conf. Ser.

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Evaluation of the functional state of hepatocytes of intact chickens and chickens under experimental klebsiellosis, escherichiosis and salmonellosis is given using luminescence spectral analysis with fluorescent dye 5-([4.6-Dichlorotriazin-2-yl]amino) fluorescein hydrochloride. A gradual increase in the proteins number in the cells was recorded from 1th to 30th days in intact chickens, which indicated an increase in the metabolism level in chicken hepatocytes. The reverse trend was observed in chickens with experimental intestinal infectious diseases.

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Application of microspectral luminescent analysis to study the intracellular metabolism in single cells and cell systems

Cited by 5 publications

References 6 publications

Effect of a Whey-Based Bioproduct on Metabolic Processes in the Microcirculation

Effect of a Whey-Based Bioproduct on Metabolic Processes in the Microcirculation

Degradation of Chlorophyll Luminescence in Plants

Microspectral method for assessing the functional state of chickens’ hepatocytes under intestinal infectious diseases

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