Correlation between the High Density Lipoprotein and its Subtypes in Coronary Heart Disease

Gao, Fen; Ren, Yanling; Shen, Xiaoyu; Bian, Yunfei; Li, Hong

doi:10.1159/000445552

Cited by 11 publications

(6 citation statements)

References 23 publications

(23 reference statements)

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“…In accordance with our results, De la Llera-Moya et al [32] reported that LPS-induced endotoxemia in healthy volunteers led to a depletion of pre-beta1a and small- and medium-sized HDL particles, determined by 2D eletrophoresis and nuclear magnetic resonance. Interestingly, small HDL particles were shown to be more antioxidant and more anti-inflammatory in cardiovascular disease [33], but large HDL particles as determined by Lipoprint technology appear to be more associated with a reduced risk of coronary artery disease [17, 18].…”

Section: Discussionmentioning

confidence: 99%

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High-density lipoprotein (HDL) particle size and concentration changes in septic shock patients

Tanaka

Diallo

Delbosc

et al. 2019

Ann. Intensive Care

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Background: Sepsis is associated with systemic inflammation that may impact lipoprotein function. In particular, high-density lipoproteins (HDLs) that display pleiotropic protective roles may be dysfunctional in septic conditions. The aim of this study was to evaluate the HDL profile and the inflammatory context in septic shock patients admitted to our intensive care unit (ICU). Methods: In this study, 20 septic shock patients and 20 controls (ICU patients without septic shock) were included. Plasma samples were collected on days 1, 2 and 7. Total cholesterol and lipoprotein concentrations were determined. HDL profiles were obtained using the Lipoprint ® System (non-denaturing electrophoresis). Quantification of pro-inflammatory cytokines (interleukin 1b, 6 and 8), cell-free DNA and lipopolysaccharide-binding protein was also performed. Results: HDL concentration was statistically lower in septic shock patients than in controls. At days 1 and 2, septic patients had significantly more large-sized HDL than control patients. Patients recovered a normal lipid profile at day 7. Conclusions: Our results emphasize that HDL levels are dramatically decreased in the acute phase of septic shock and that there is a shift toward large HDL particles, which may reflect a major dysfunction of these lipoproteins. Further mechanistic studies are required to explore this shift observed during sepsis.

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Section: Discussionmentioning

confidence: 99%

“…In the cardiovascular field, both HDL cholesterol (HDL-C) concentration and HDL particle size were independently associated with cardiovascular risk [16]. In particular, large HDL particles appear to be protective in coronary artery disease [17, 18]. To our knowledge, HDL size has never been explored in septic patients.…”

Section: Introductionmentioning

confidence: 99%

High-density lipoprotein (HDL) particle size and concentration changes in septic shock patients

Tanaka

Diallo

Delbosc

et al. 2019

Ann. Intensive Care

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“…It is now accepted that the importance of HDL in the progression of CVD, resides on their quality rather than their quantity, highlighting the importance of their composition, structure, and function [10,11,12,13]. Indeed, apolipoprotein A–I (Apo A–I), the major protein component of HDL, has cardiovascular protective properties [14,15]. Also, low levels of apolipoprotein E (Apo E) have been related to hyperlipidemia and atherosclerosis [16].…”

Section: Introductionmentioning

confidence: 99%

Phytosterols and Omega 3 Supplementation Exert Novel Regulatory Effects on Metabolic and Inflammatory Pathways: A Proteomic Study

Lambert¹,

Cubedo

Padró

et al. 2017

Nutrients

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Cardiovascular disease (CVD) remains one of the major causes of death and disability worldwide. In addition to drug treatment, nutritional interventions or supplementations are becoming a health strategy for CVD prevention. Phytosterols (PhyS) are natural components that have been shown to reduce cholesterol levels; while poly-unsaturated fatty acids (PUFA), mainly omega-3 (ω3) fatty acids, have shown to reduce triglyceride levels. Here we aimed to investigate whether the proteins in the main lipoproteins (low density lipoproteins (LDL) and high density lipoproteins (HDL)) as well as proteins in the lipid free plasma fraction (LPDP) were regulated by the intake of PhyS-milk or ω3-milk, in overweight healthy volunteers by a proteomic based systems biology approach. The study was a longitudinal crossover trial, including thirty-two healthy volunteers with body mass index (BMI) 25–35 kg/m2 (Clinical Trial: ISRCTN78753338). Basal samples before any intervention and after 4 weeks of intake of PhyS or ω3-milk were analyzed. Proteomic profiling by two dimensional electrophoresis (2-DE) followed by mass spectrometry-(MALDI/TOF), ELISA, Western blot, conventional biochemical analysis, and in-silico bioinformatics were performed. The intake of PhyS-milk did not induce changes in the lipid associated plasma protein fraction, whereas ω3-milk significantly increased apolipoprotein (Apo)- E LDL content (p = 0.043) and induced a coordinated increase in several HDL-associated proteins, Apo A–I, lecitin cholesterol acyltransferase (LCAT), paraoxonase-1 (PON-1), Apo D, and Apo L1 (p < 0.05 for all). Interestingly, PhyS-milk intake induced a reduction in inflammatory molecules not seen after ω3-milk intake. Serum amyloid P component (SAP) was reduced in the LPDP protein fraction (p = 0.001) of subjects taking PhyS-milk and C-C motif chemokine 2 (CCL2)expression detected by reverse transcription polymerase chain reaction (RT-PCR) analysis in white blood cells was significantly reduced (p = 0.013). No changes were observed in the lipid-free plasma proteome with ω3-milk. Our study provides novel results and highlights that the PhyS-milk induces attenuation of the pro-inflammatory pathways, whereas ω3-milk induces improvement in lipid metabolic pathways.

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“…SAA has been shown to displace some of the primary protein component of HDL, apolipoprotein A‐I (ApoA‐I), during acute inflammation, resulting in altered HDL functionality (O'Brien & Chait, 2006 ; Bindu et al, 2011 ). The presence of SAA has been shown to reduce the normal anti‐inflammatory activity of HDL (Jahangiri et al, 2013 ; Gao et al, 2016 ). This leads to the activation of proinflammatory and proatherogenic pathways through increased interactions with toll‐like receptors 2, and 4 (TLR‐2 and TLR‐4) to promote the release of proinflammatory cytokines, FPRL‐1 to promote chemotaxis towards the site of infection/injury, and CD36 to promote lipid uptake (Buck et al, 2016 ; Bindu et al, 2011 ; Eklund et al, 2012 ; Migita et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Structural studies of a serum amyloid A octamer that is primed to scaffold lipid nanodiscs

Nady,

Reichheld,

Sharpe

2024

Protein Science

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Serum amyloid A (SAA) is a highly conserved acute‐phase protein that plays roles in activating multiple pro‐inflammatory pathways during the acute inflammatory response and is commonly used as a biomarker of inflammation. It has been linked to beneficial roles in tissue repair through improved clearance of lipids and cholesterol from sites of damage. In patients with chronic inflammatory diseases, elevated levels of SAA may contribute to increased severity of the underlying condition. The majority of circulating SAA is bound to lipoproteins, primarily high‐density lipoprotein (HDL). Interaction with HDL not only stabilizes SAA but also alters its functional properties, likely through altered accessibility of protein–protein interaction sites on SAA. While high‐resolution structures for lipid‐free, or apo‐, forms of SAA have been reported, their relationship with the HDL‐bound form of the protein, and with other possible mechanisms of SAA binding to lipids, has not been established. Here, we have used multiple biophysical techniques, including SAXS, TEM, SEC‐MALS, native gel electrophoresis, glutaraldehyde crosslinking, and trypsin digestion to characterize the lipid‐free and lipid‐bound forms of SAA. The SAXS and TEM data show the presence of soluble octamers of SAA with structural similarity to the ring‐like structures reported for lipid‐free ApoA‐I. These SAA octamers represent a previously uncharacterized structure for lipid‐free SAA and are capable of scaffolding lipid nanodiscs with similar morphology to those formed by ApoA‐I. The SAA–lipid nanodiscs contain four SAA molecules and have similar exterior dimensions as the lipid‐free SAA octamer, suggesting that relatively few conformational rearrangements may be required to allow SAA interactions with lipid‐containing particles such as HDL. This study suggests a new model for SAA–lipid interactions and provides new insight into how SAA might stabilize protein‐lipid nanodiscs or even replace ApoA‐I as a scaffold for HDL particles during inflammation.

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Correlation between the High Density Lipoprotein and its Subtypes in Coronary Heart Disease

Cited by 11 publications

References 23 publications

High-density lipoprotein (HDL) particle size and concentration changes in septic shock patients

High-density lipoprotein (HDL) particle size and concentration changes in septic shock patients

Phytosterols and Omega 3 Supplementation Exert Novel Regulatory Effects on Metabolic and Inflammatory Pathways: A Proteomic Study

Structural studies of a serum amyloid A octamer that is primed to scaffold lipid nanodiscs

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