Correlation between surface friction and the hydrophobicity of structure-related side-chain exposure of albumin on contact lens

Huang, Tzu-Yin; Chang, Che‐Wei; Baskaran, Nareshkumar; Wei, Yang

doi:10.1016/j.colsurfb.2021.112152

Cited by 4 publications

(3 citation statements)

References 67 publications

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“…For example, model pHEMA and polymethyl methacrylate (PMMA) lens surfaces became more hydrophobic (less wettable) upon adsorption of albumin. 44 COF increased concomitantly at high surface coverage but was not significantly different at lower coverage when the protein denatured. 44 In comparison, when lysozyme sorbed on CLs, COF increased at high surface coverage of ocufilcon D and helfilcon A but not nelfilcon A lenses.…”

Section: Discussionmentioning

confidence: 87%

“… 44 COF increased concomitantly at high surface coverage but was not significantly different at lower coverage when the protein denatured. 44 In comparison, when lysozyme sorbed on CLs, COF increased at high surface coverage of ocufilcon D and helfilcon A but not nelfilcon A lenses. 45 The researchers proposed that increased COF might be related to denatured lysozyme rather than total lysozyme sorbed.…”

Section: Discussionmentioning

confidence: 87%

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Effect of Contact Lens Solutions in Stabilizing the Activity of Tear Lysozyme

Scheuer¹,

Barniak²,

Phatak³

et al. 2023

OPTO

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Purpose Interactions between tear proteins and the interfaces of contact lenses can be complex and can influence contact lens wear success. Tear proteins, including lysozyme, function to maintain the balance of ocular surface homeostasis, as evidenced by the effects of its conformation relative to stabilizing the tear film and its potential impact on corneal epithelial cells. Contact lens manufacturers include components in lens care and blister package solutions to help stabilize the tear film and preserve homeostasis. This in vitro study was performed to evaluate the ability of daily disposable contact lens package solutions to stabilize lysozyme and preserve its native conformation under denaturing conditions. Methods Lysozyme was added to contact lens solutions sampled from kalifilcon A, etafilcon A, senofilcon A, narafilcon A, nelfilcon A, verofilcon A, delefilcon A, somofilcon A, and stenfilcon A blister packages, then mixed with the protein denaturant sodium lauryl sulfate. Lysozyme activity was evaluated by adding test solutions to a suspension of Micrococcus luteus . Native lysozyme lyses the Micrococcus luteus cell wall, which decreases suspension turbidity. Stabilization of lysozyme activity was determined by comparing suspension turbidity before and after exposure to test solutions. Results Lysozyme stabilization was 90.7% for kalifilcon A solution, a statistically significant improvement (p < 0.05) compared to phosphate buffered saline (PBS, negative control). No significant improvement was observed with any other contact lens solution (all lysozyme stabilization < 5.00%). Conclusion The representative tear protein lysozyme was significantly more stable in the novel kalifilcon A contact lens solution containing multiple moisturizers and osmoprotectants than in PBS or other daily disposable contact lens solutions. The lysozyme activity assay provides mechanistic evidence that the kalifilcon A contact lens solution can stabilize proteins under conditions that typically denature proteins, which may contribute to maintaining ocular surface homeostasis.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 87%

Effect of Contact Lens Solutions in Stabilizing the Activity of Tear Lysozyme

Scheuer¹,

Barniak²,

Phatak³

et al. 2023

OPTO

View full text Add to dashboard Cite

show abstract

“…The hydrophobicity of the material surface is one of the main factors that induce protein adsorption on CI electrodes. [36,37] To determine the effects of hydrophilic modulation on protein adsorption, BSA and FBS were used to examine the antifouling properties of the PSB-PDA coating. As shown by the confocal microscopy images in Figure 2C, BSA-FITC was rarely observed on the surface of PSB-PDA coated silicone wafers compared with the uncoated control.…”

Section: Resultsmentioning

confidence: 99%

Zwitterionic Polymer/Polydopamine Coating of Electrode Arrays Reduces Fibrosis and Residual Hearing Loss after Cochlear Implantation

Chen

et al. 2022

Adv Healthcare Materials

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Since the first surgery 50 years ago, cochlear implantation (CI) is the major treatment for patients with severe sensorineural hearing loss. However, unexpected foreign body reactions (FBRs) after surgery are reported in 90% of CI recipients, resulting in the formation of fibrosis in the cochlea and progressive residual hearing loss. Zwitterion modification is universally used to reduce bio‐fouling and suppress FBRs but never for CI. In the present study, a zwitterionic coating is developed, which is composed of poly sulfobetaine methacrylate (PSB) and polydopamine (PDA) for cochlear implants. The PSB‐PDA coating shows a series of characters for an ideal anti‐FBRs material, including super‐hydrophilicity, low protein and cell adsorption, long‐term stability, and high biocompatibility. Compared to the uncoated controls, PSB‐PDA coating inhibits the activation of macrophages and reduces the release of inflammatory factors (TNF‐α, IL‐1β, NO) and fibrosis‐related factors (TGF‐β1, α‐SMA, collagen I). PSB‐PDA coated electrode arrays suppress fibrosis completely and preserve residual hearing significantly in rat CI models. These results suggest that PSB‐PDA coating is a novel strategy for anti‐fibrosis to improve the outcomes of CI.

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