Correlation between sperm motility and sperm chromatin structure assay parameters

Giwercman, Aleksander; Richthoff, Jonas; Hjøllund, Henrik I.; Bonde, Jens Peter; Jepson, K; Frohm, Birgitta; Spanò, Marcello

doi:10.1016/s0015-0282(03)02212-x

Cited by 182 publications

(121 citation statements)

References 44 publications

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“…The formation of a mature sperm nucleus, which is characterized by the replacement of nuclear histones with protamines, and the development of the sperm flagellum both originate during spermiogenesis. In agreement with our findings, several investigators have reported that sperm % DFI is associated with sperm motility [11,16,20,27,33]. In contrast, others have reported either an absence of any meaningful correlation between SCSA parameters and World Health Organization (WHO) parameters [13] or have observed only a weak correlation between these parameters [23].…”

Section: Discussionsupporting

confidence: 91%

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High prevalence of isolated sperm DNA damage in infertile men with advanced paternal age

Das

Alhathal

San-Gabriel

et al. 2013

J Assist Reprod Genet

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Background Sperm DNA damage is associated with male infertility, lower pregnancy rates and pregnancy loss. Objective The primary aim of our study was to evaluate the prevalence of sperm DNA damage in younger and older men with normozoospermia. Design, Setting and Participants We obtained semen from 277 consecutive non-azoospermic men presenting for sperm DNA testing. Outcome Measurements and Statistical Analysis The main outcome measures included sperm % DNA fragmentation index (%DFI, using sperm chromatin structure assay), sperm concentration, motility and morphology, and, paternal age.Results and Limitations Sperm % DFI was positively correlated with paternal age (r=0.20, P<0.001) and inversely correlated % progressive motility (r=−0.16, P=0.01). Sperm %DFI was significantly higher in older (≥40 years) compared to younger (<40 years) normozoospermic men (17±13 vs. 12±8, respectively P=0.008), whereas, sperm concentration, progressive motility and morphology were not significantly different in these two groups. Moreover, the prevalence of high levels of sperm DNA damage (>30 % DFI) was significantly higher in older compared to younger normozoospermic men (17 % vs. 3 %, respectively, P<0.001). Conclusion The data indicate that a conventional semen analysis can often fail to detect a defect in spermatogenesis (high %DFI) in older men and suggest that infertile couples with advanced paternal age, including those with normal semen parameters, should consider sperm DNA testing as part of the couple evaluation.

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Section: Discussionsupporting

confidence: 91%

“…Several studies have also demonstrated that sperm DNA damage is associated with advanced paternal age and poor semen parameters [11,16,26,27,34]. Increased levels of sperm DNA damage have been linked with chromosomal abnormalities, developmental loss and birth defects in mouse model systems [18,24].…”

Section: Introductionmentioning

confidence: 99%

High prevalence of isolated sperm DNA damage in infertile men with advanced paternal age

Das

Alhathal

San-Gabriel

et al. 2013

J Assist Reprod Genet

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“…Researchers have attributed this phenomenon to a reduction in the spermatid and spermatozoa numbers, caused by a failure of the completion of the spermatocyte cycle (Rockett et al 2001;Giwercman et al 2003). Results from our studies agreed with the preliminary findings with mice, rats (Setchell et al 1998;Aktas and Kanter 2009) and some other species (Kowalowka et al 2008) characterized by the reduced percentage of acrosomal integrity andlive sperms, sperm concentration and motility, as well as the percentage of fertility, and the increased rate of abnormal sperm (P B0.05).…”

Section: Discussionsupporting

confidence: 90%

Effects of a mild heat treatment on mouse testicular gene expression and sperm quality

Zhao

Zhang

Hao

et al. 2010

Animal Cells and Systems

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The decrease in sperm quality under heat stress causes a great loss in animal husbandry production. In order to reveal the mechanism underlying the sperm quality decrease caused by heat stress, we first established a mild heattreated mouse model. Then, the sperm quality was identified. Further, the testicular proteome profile was mapped and compared with the control using 2D electrophoresis and mass spectrometry. Finally, the differential expressed proteins involved in the heat stress response were identified by real-time PCR and Western blotting. The results showed that heat stress caused a significant reduction in mouse sperm quality (P B 0.05). Further, 52 protein spots on the 2D gel were found to differ between the heat-shocked tissues and the control. Of these spots, some repair proteins which might provide some explanation for the influence on sperm quality were found. We then focused on Bag-1, Hsp40, Hsp60 and Hsp70, which were found to be differently expressed after heat shock (P B 0.05). Further analysis in this heat-shocked model suggests numerous potential mechanisms for heat shock-induced spermatogenic disorders.

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“…Moreover, in the subgroups of men with isolated asthenozoospermia and those with isolated teratozoospermia, sperm DNA fragmentation is inversely related to sperm progressive motility but is not related to sperm concentration or morphology. Taken together, these data are in keeping with earlier observations demonstrating a significant relationship between sperm DNA damage and sperm motility [20,21,[29][30][31][32][33][34][35]. The underlying nature of the relationship between sperm DNA damage and sperm motility has not been fully characterized.…”

Section: Discussionsupporting

confidence: 83%

Which isolated sperm abnormality is most related to sperm DNA damage in men presenting for infertility evaluation

Belloc¹,

Benkhalifa²,

Cohen-Bacrie³

et al. 2014

J Assist Reprod Genet

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Background Sperm DNA damage is common in infertile men and is associated with poor semen parameters but the impact of an isolated sperm abnormality on sperm DNA damage has not been studied. Objective To evaluate sperm DNA damage in a large cohort of infertile men with isolated sperm defects. Design, setting and participants Retrospective study of 1084 consecutive, non-azoospermic infertile men with an isolated sperm defect: isolated oligozoospermia (iOligo), isolated asthenozoospermia (iAstheno) or isolated teratozoospermia (iTerato). Outcome measurements and statistical analysis We examined and compared clinical parameters, conventional semen parameters and %sperm DNA fragmentation (%SDF, assessed by flow cytometry-based Terminal deoxynucleotidyl transferase-mediated dUTP Nick End-Labeling assay) in the three groups of men. Results and limitations The mean (±SD) %SDF was significantly higher in the iAstheno compared to the iOligo and iTerato groups (25.0±14.0 vs. 19.2±11.6 and 20.7±12.1 %, respectively, P<0.0001). Similarly, the proportion of men with high %SDF (>30 %) was significantly higher in the iAstheno compared to the iOligo and iTerato groups (31 % vs. 18 % and 19 %, respectively, P<0.0001). In the group of 713 men with iAstheno, %SDF was positively correlated with paternal age (r=0.20, P<0.0001) and inversely correlated with %progressive motility (r=−0.18, P<0.0001). In the subset of 218 men with iTerato, %SDF was also positively correlated with paternal age (r=0.15, P=0.018) and inversely correlated with %progressive motility (r=−0.26, P=0.0001). Conclusions In this large cohort of infertile men with isolated sperm abnormalities, we have found that the sperm DNA fragmentation level is highest in the men with sperm motility defects and that 31 % of these men have high levels of sperm DNA fragmentation. The data indicate that poor motility is the sperm parameter abnormality most closely related to sperm DNA damage.

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Correlation between sperm motility and sperm chromatin structure assay parameters

Cited by 182 publications

References 44 publications

High prevalence of isolated sperm DNA damage in infertile men with advanced paternal age

High prevalence of isolated sperm DNA damage in infertile men with advanced paternal age

Effects of a mild heat treatment on mouse testicular gene expression and sperm quality

Which isolated sperm abnormality is most related to sperm DNA damage in men presenting for infertility evaluation

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