Correlation between sheep YAP1 temporal and spatial expression trends and MSTN and MyoG gene expression

Lv, Xiaoyang; Sun, Wei; Su, Rui; Li, D.; Wang, Q.Z.; Musa, H H; Chen, L.; Zhang, Y.F.; Wu, Wenbo

doi:10.4238/2015.april.13.3

Cited by 9 publications

(5 citation statements)

References 8 publications

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“…One potential explanation is that the functions of miR-29a differ between species. In addition, several studies have revealed similar expression profiles between MyoG (Lv et al, 2015), MyHC (Gao et al, 2016), and YAP1 (decreases with age), indicating that miR-29a may inhibit both YAP1 and genes related to differentiation in Hu sheep SMSCs. Another potential explanation is that, in addition to YAP1, there might be other genes targeted by miR-29a involved in the proliferation and differentiation of SMSCs.…”

Section: Discussionmentioning

confidence: 85%

Long Noncoding RNA (lncRNA) CTTN-IT1 Elevates Skeletal Muscle Satellite Cell Proliferation and Differentiation by Acting as ceRNA for YAP1 Through Absorbing miR-29a in Hu Sheep

Wang

et al. 2020

Front. Genet.

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Characterizing the factors that regulate the growth and development of muscle is central to animal production. Skeletal muscle satellite cells (SMSCs) provide an important material for simulating the proliferation and differentiation of muscle cells. YAP1, which can promote muscle growth, is closely related to the proliferation of SMSCs in Hu sheep (Ovis aries). In addition, some miRNAs, such as miR-541-3p, miR-142-5p, and miR-29a, can play critical roles in muscle growth by specifically binding with their target mRNAs. Meanwhile, lncRNA can competitively bind these miRNAs and reduce the regulatory effect of miRNAs on their target genes and thus play critical roles themselves in muscle growth. However, the regulatory molecular mechanism of miRNA and lncRNA on SMSC proliferation through YAP1 remains unclear. Here, we characterized the regulatory network among YAP1 and its targeted miRNAs and lncRNAs in Hu sheep SMSCs. The potential ncRNAs that regulate YAP1 (miR-29a and CTTN-IT1) were predicted through multilevel bioinformatics analysis. Dual-luciferase assays, RT-qPCR, and western blots revealed that miR-29a can significantly reduce the mRNA and protein expression level by binding to a specific 3-UTR of YAP1 (P < 0.05), while CTTN-IT1 can restore the expression of YAP1 through competitive binding to miR-29a. Furthermore, the mRNA and protein expression levels of MyoG, MyoD, and MyHC showed that miR-29a can inhibit the expression of genes related to the differentiation of SMSCs, and CTTN-IT1 can increase the expression of these same genes. Thus, miR-29a may inhibit the differentiation of SMSCs and CTTN-IT1 can restore this inhibition. The EdU staining assay indicated that excessive miR-29a can significantly reduce the proliferation ability of SMSCs (P < 0.05), while overexpression of CTTN-IT1 can significantly increase the proliferation of SMSCs (P < 0.01). CTTN-IT1 is a novel lncRNA that is a competing

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Section: Discussionmentioning

confidence: 85%

Long Noncoding RNA (lncRNA) CTTN-IT1 Elevates Skeletal Muscle Satellite Cell Proliferation and Differentiation by Acting as ceRNA for YAP1 Through Absorbing miR-29a in Hu Sheep

Wang

et al. 2020

Front. Genet.

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“…Zhang et al [38] studied the expression of the myogenin gene in chickens and showed that this gene expresses in the breast muscle, leg muscle, heart, liver, spleen, lung, kidney, glandular stomach, and ovarian tissues. Lv et al [39] and Forutan et al [40] demonstrated that the myogenin gene expresses in different skeletal muscles of sheep, such as the soleus, gastrocnemius, longissimus dorsi, and extensor digitorum longus muscles. Kuang et al [41] reported the expression of the myogenin gene in the skeletal muscles of rabbits.…”

Section: Discussionmentioning

confidence: 99%

An Origanum majorana Leaf Diet Influences Myogenin Gene Expression, Performance, and Carcass Characteristics in Lambs

Safaei

Dadpasand

Mohammadabadi

et al. 2022

Animals

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Myogenin is a protein in the myogenic regulatory factor family that plays an important role in determining carcass and meat traits and is vital for the growth and development of livestock muscles. The objective of this study was to determine the impact of Origanum majorana leaf (MOL) on the myogenin gene expression of lambs. Twenty-four male Kermani lambs were used in a completely randomized design using two experimental groups (0% Origanum majorana L. = MOL0 and 4% Origanum majorana L. = MOL4). Final weight, average daily gain, hot and cold carcass weight, feed conversion ratio, empty body weight, hot and cold dressing percentage, the weight of the shoulder, loin, leg, and lean meat, and the lean/bone ratio were measured. A standard kit was used for extracting total RNA from the loin, leg, and shoulder muscles of the lambs’ tissues. The cDNA was synthesized, a real-time PCR was performed, and the obtained data were analyzed. The results of this study showed that the effect of MOL4 on dry matter intake is not significant. The MOL4 diet increased final weight by 8.22%, average daily gain by 28.57%, hot carcass weight by 11.38%, cold carcass weight by 13.43%, feed conversion ratio by 31.03%, empty body weight by 9.38%, hot dressing percentage by 2.92%, cold dressing percentage by 3.75%, shoulder weight by 56.70%, loin weight by 8.98%, leg weight by 10.90%, lean meat weight by 14.62%, and the lean/bone ratio by 2.85% (p < 0.05) compared to the MOL0. Along with adding MOL4 in the lambs’ diets, in comparison with MOL0, there was higher expression of myogenin in the loin (3.5 times), leg (3.9 times), and shoulder (3.6 times) muscles of the lambs. Due to the fact that adding Origanum majorana to the diet of the lambs enhanced the expression of the myogenin gene in the loin, leg, and shoulder muscles and increased parameters related to growth, it can be used to improve the parameters related to growth and to increase myogenin gene expression in the muscle of lambs.

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“…(Siqin et al, 2017) reported that the MYOG and MYF6 gene expression levels in the LD muscle were stable during postnatal development in the fat-tailed Wuzhumuqin sheep breed. A study investigating these effects on muscle development in short-tailed Hu sheep found that the expression level of the MYOG gene was low (down-regulated) in the LD muscle, whereas the expression level was high (upregulated) in the gastrocnemius and soleus muscles, reporting the dependence of the expression level of the MYOG gene on the muscle groups as one of the reasons for this difference (Lv et al, 2015). In support of this view, this study found that the MYOG gene and protein expression level was statistically signi cantly (P < 0,05) high (up-regulated) in the LD muscle in the thin-tailed Karayaka sheep breed, while the expression level of the same gene and protein was low in the GL muscle (P < 0,05).…”

Section: Discussionmentioning

confidence: 99%

Investigation of Expression Levels of Transcription Factor Genes in Native Sheep Breeds of Turkey

Arslan

Daldaban

Bayram

et al. 2022

Preprint

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This study, it was aimed to investigate the expression levels of transcription factor (TF) genes (MYF6, MYOD1, MYF5, MYOG) and proteins associated with muscle growth in the longissimus dorsi (LD) and gluteal (GL) muscles in sheep. For this purpose, two fat-tailed sheep breeds (Akkaraman (n = 10), İvesi (n = 10)) and two thin-tailed sheep breeds (Kivircik (n = 10) and Karayaka (n = 10)) from Turkey's native sheep breeds were examined. The expression level of RNAs and proteins isolated from fresh tissues and MYF6, MYOD1, MYF5, MYOG proteins were analyzed. As a result of the statistical analysis, in the LD tissue, respectively, MYOG and MYF5 genes in the Karayaka sheep breed; MYOD1 gene in Akkaraman sheep breed; MYF5 gene in Awassi sheep breed were found to be statistically significant (P < 0.05). In GL tissue, respectively, MYOG and MYF6 genes in Akkaraman sheep breed; MYOD1 gene in Karayaka sheep breed; MYF6 gene in Akkaraman and Awassi sheep breed were found to be statistically significant (P < 0.05). In the present study, it was found that the MYOG (fold change 6.87) and MYOD1 (fold change 15.41) genes were upregulated in the GL muscle of the fat-tailed Akkaraman sheep breed. In addition, in the thin-tailed Karayaka sheep breed, down-regulation of MYOD1 (fold change − 0.22) gene in LD muscle and up-regulation of MYOD1 (fold change 6.67) gene in GL muscle was found. As a result, it can be considered that MYOG and MYOD1 genes as candidate genes in molecular selection studies for our fat-tailed and thin-tailed indigenous breeds in terms of muscle development.

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Correlation between sheep YAP1 temporal and spatial expression trends and MSTN and MyoG gene expression

Cited by 9 publications

References 8 publications

Long Noncoding RNA (lncRNA) CTTN-IT1 Elevates Skeletal Muscle Satellite Cell Proliferation and Differentiation by Acting as ceRNA for YAP1 Through Absorbing miR-29a in Hu Sheep

Long Noncoding RNA (lncRNA) CTTN-IT1 Elevates Skeletal Muscle Satellite Cell Proliferation and Differentiation by Acting as ceRNA for YAP1 Through Absorbing miR-29a in Hu Sheep

An Origanum majorana Leaf Diet Influences Myogenin Gene Expression, Performance, and Carcass Characteristics in Lambs

Investigation of Expression Levels of Transcription Factor Genes in Native Sheep Breeds of Turkey

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