Correlation Between qacE and qacE∆1 Efflux Pump Genes, Antibiotic and Disinfectant Resistant Among Clinical Isolates of E.coli

Shafaati, Maryam; Boroumand, Mohammadali; Nowroozi, Jamileh; Amiri, Pouya; Kazemian, Hossein

doi:10.2174/1574891x11666160815094718

Cited by 21 publications

(15 citation statements)

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“…This, alongside the broad range of substrates (QAC compounds, disinfectants, antibiotics, etc.) that SMRs can extrude and their increasing prevalence on plasmids and integrons in clinical and multidrug-resistant strains of bacteria (13,(27)(28)(29)(30)(31)(32)(33)(34)(35), marks these proteins as important therapeutic targets.…”

Section: Discussionmentioning

confidence: 99%

Peptide-Based Efflux Pump Inhibitors of the Small Multidrug Resistance Protein from Pseudomonas aeruginosa

Mitchell

Stone

Deber

2019

Antimicrob Agents Chemother

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Bacteria have acquired multiple mechanisms to evade the lethal effects of current therapeutics, hindering treatment of bacterial infections, such as those caused by the pathogen Pseudomonas aeruginosa, which is responsible for nosocomial and cystic fibrosis lung infections. One resistance mechanism involves membrane-embedded multidrug efflux pumps that can effectively extrude an array of substrates, including common antibiotics, dyes, and biocides. Among these is a small multidrug resistance (SMR) efflux protein, consisting of four transmembrane (TM) helices, that functions as an antiparallel dimer. TM helices 1 to 3 (TM1 to TM3) comprise the substrate binding pocket, while TM4 contains a GG7 heptad sequence motif that mediates the SMR TM4-TM4 dimerization. In the present work, we synthesized a series of peptides containing the residues centered on the TM4-TM4 binding interface found in the P. aeruginosa SMR (PAsmr), typified by Ac-Ala-(Sar)3-LLGIGLIIAGVLV-KKK-NH2 (helix-helix interaction residues are underlined). Here, the acetylated N-terminal sarcosine (N-methyl-Gly) tag [Ac-Ala-(Sar)3] promotes membrane penetration, while the C-terminal Lys tag promotes selectivity for the negatively charged bacterial membranes. This peptide was observed to competitively disrupt PAsmr-mediated efflux, as measured by efflux inhibition of the fluorescent dye ethidium bromide, while having no effect on cell membrane integrity. Alternatively, a corresponding peptide in which the TM4 binding motif is scrambled was inactive in this assay. In addition, when Escherichia coli cells expressing PAsmr were combined with sublethal concentrations of several biocides, growth was significantly inhibited when peptide was added, notably, by up to 95% with the disinfectant benzylalkonium chloride. These results demonstrate promise for an efflux pump inhibitor to address the increasing threat of antibiotic-resistant bacteria.

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Section: Discussionmentioning

confidence: 99%

Peptide-Based Efflux Pump Inhibitors of the Small Multidrug Resistance Protein from Pseudomonas aeruginosa

Mitchell

Stone

Deber

2019

Antimicrob Agents Chemother

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“…Previously, a relationship between the presence of qac gene and increased MIC in A. baumannii isolates was reported 33 . MICs were suggested to be significantly high in strains of Salmonella spp., Escherichia coli , Klebsiella pneumoniae , and Staphylococcus aureus containing the qacEΔ1 gene 25,34 . In contrast, there are other studies that do not suggest a significant difference between the presence or absence of qacE gene and susceptible/resistant strains of A. baumannii 18,23 …”

Section: Discussionmentioning

confidence: 99%

Upregulation of abeM, amvA, and qacEΔ1 efflux pump genes associated with resistance of Acinetobacter baumannii strains to disinfectants

Rostami

Ranjbar

Ghourchian

et al. 2021

Health Science Reports

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Background and aims Acinetobacter baumannii is among the most concerning cause of nosocomial infections due to its high level of antibiotic resistance and high mortality. The aim of this study was to determine the role of efflux pumps in resistance of A. baumannii strains to three disinfectants, including MICROZED ID‐MAX, NANOSIL D2, and OPIDEX OPA. Methods Twenty‐eight environmental and clinical isolates of A. baumannii were collected from selected hospitals of central Iran. The minimum inhibitory concentrations of the disinfectants were determined and real time reverse transcriptase‐PCR was performed to investigate the expression level of qacEΔ1 , amvA , abeM , and adeB efflux pump genes. Results Considering both clinical and environmental isolates, there was a significant difference in the mean expression level of qacEΔ1 gene between susceptible and resistant strains to MICROZED ID‐MAX disinfectant, of amvA and abeM genes between susceptible and resistant strains to NANOSIL D2 disinfectant and of abeM gene in susceptible and resistant strains to OPIDEX OPA disinfectant (all P ˂ .05). The expression levels of abeM and amvA genes were higher in the environmental isolates that were resistant to NANOSIL D2 disinfectant compared to those that were susceptible ( P ˂ .05). Conclusions This study provided evidence for the role of abeM and amvA genes in the resistance of environmental isolates to disinfectants, particularly hydrogen peroxide derivatives.

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“…12 Sin embargo, esa situación ha ido en aumento, en el Cuadro 1 se detallan algunos informes de aislamientos bacterianos resistentes a desinfectantes a nivel clínico alrededor del mundo, siendo de particular interés el incremento en la frecuencia de estos en los últimos 5 años. [13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] Es necesario destacar que un aislamiento bacteriano se considera resistente cuando la concentración mínima inhibitoria que presenta es superior a la concentración de trabajo del biocida respectivo, de acuerdo con las recomendaciones del fabricante, o en su defecto, del protocolo de aplicación de estas sustancias en el centro médico donde se realizó el aislamiento.…”

Section: Resistencia a Biocidasunclassified

Resistencia a desinfectantes y su relación con la resistencia a los antibióticos

Jiménez¹,

Jiménez²

2020

AMC

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Objetivo: sistematizar los principales mecanismos de acción de los desinfectantes y describir mecanismos de resistencia comunes entre biocidas y antibióticos. Métodos: se realizó una revisión bibliográfica de artículos científicos entre 2000 y julio de 2019, sobre la relación entre la resistencia a biocidas y a los antibióticos, utilizando como palabras clave “antibiotic”, “biocide”, “resistance” y “bacteria”, en las bases de datos PubMed y Google Scholar. Conclusiones: existe numerosa evidencia científica que indica la relación entre bacterias resistentes a los desinfectantes y la adquisición de resistencia a los antibióticos, lo que implica un replanteamiento del uso de esas sustancias en diferentes contextos clínicos, con el fin de minimizar el impacto que puedan tener en la selección de microorganismos multirresistentes a los antibióticos. Descriptores: bacterias, desinfectantes, resistencia, antibióticos.

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Correlation Between qacE and qacE∆1 Efflux Pump Genes, Antibiotic and Disinfectant Resistant Among Clinical Isolates of E.coli

Cited by 21 publications

References 0 publications

Peptide-Based Efflux Pump Inhibitors of the Small Multidrug Resistance Protein from Pseudomonas aeruginosa

Peptide-Based Efflux Pump Inhibitors of the Small Multidrug Resistance Protein from Pseudomonas aeruginosa

Upregulation of abeM, amvA, and qacEΔ1 efflux pump genes associated with resistance of Acinetobacter baumannii strains to disinfectants

Resistencia a desinfectantes y su relación con la resistencia a los antibióticos

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