Correlation between PTEN and P62 gene expression in rat colorectal cancer cell

Abstract

ObjectiveAutophagy is a cellular pathway that regulates the transportation and degradation of cytoplasmic macromolecules and organelles towards lysosome, which is often related to the tumorigenesis and tumor suppression. Here, we investigate the regulating effect of PTEN gene on autophagy-related protein P62 in rat colorectal cancer (CRC) cells and explore the application value of PTEN gene in clinic.MethodsRat colorectal cancer was induced by intraperitoneal injection of 1,2-dimethyl hydrazine in male ACI rats. A total of 20 rats were randomly selected from those successfully induced with CRC as the experimental group, while 10 healthy rats as control. The rat CRC cells were isolated and cultured. After transfecting the rat CRC cells with pEGFP-N1-PTEN plasmid, RT-PCR was adopted to examine that gene expression of p62 and PTEN, while Western blotting was used to detect the protein expression of p62 and PTEN. Also, the proliferation of CRC cells was measured by MTT assay.ResultsThe expression of PTEN gene in the experimental group was significantly inhibited as compared with the control group, while the expression of P62 gene was significantly increased (p < 0.05). Western blotting demonstrated that the PTEN protein in the experimental group was lower, while the expression of P62 protein was higher. When the CRC cells were transfected with pEGFP-N1-PTEN plasmid, the PTEN expressions were elevated, while p62 was down-regulated. Also, the proliferation of CRC cells was inhibited.ConclusionThe expression of PTEN gene is negatively correlated with the expression of P62 gene in rat CRC cells. And the expression of PTEN gene can inhibit the occurrence and development of colorectal cancer, thus providing theoretical basis for future clinical treatment.

Antitumor activity of 5-fluorouracil polymeric nanogel synthesized by gamma radiation on a rat model of colon carcinoma: a proposed mechanism

“…Activation of mTOR and inhibition of autophagy was associated with abnormal accumulation of p62 in cancerous tissues [ 61 ]. Coinciding with this, the results of Zhang et al [ 62 ] revealed a dramatic elevation of the p62 expression in colon cancer tissues. In addition to suppressing autophagy, Brech et al [ 63 ] observed that activation of mTOR and AKT inhibited cellular apoptosis via phosphorylation of caspase-3 and caspase-9.…”

Classification of Potential Breast/Colorectal Cancer Cases Using Machine Learning Methods

“…Another study revealed that SFRP1 gene methylation in CRC was associated with lymph node invasion (50). On the other hand, PTEN has been widely studied as an indicator of CRC (18). A deeper look at the Tables 1 and 2 shows that relying on the set of genes that have already been identified as tumor markers (i.e.…”

“…Relevant studies on these genes concluded that they could develop breast cancer (10)(11)(12)(13)(14)(15)(16)(17). Similarly, some genes, including PTEN, P62, NOD2, TP53, MSH3, POFUT1, RPRD1B, EIF6, MGP, FGF2, OGDHL, CYP24A1, WNT1, KLF5, WNT16, CLDN1, and TET3 have been effective in CRC development (18)(19)(20)(21)(22)(23)(24)(25). Analyzing any change in the expression of the genes can help in understanding the main causes of the disease and reveals at which state the disease is and how to handle it and plan proper and efficient treatments in numerous fields of application, such as drug designs (26) and personalized medicines (27).…”

An Episomal CRISPR/Cas12a System for Mediating Efficient Gene Editing

“…Additionally, AsCas12a and the U6 promoter were successively cloned into the pCEP4 vector (Thermo Fisher, Waltham, MA, USA) with T4 ligase (M0569S, New England Biolabs) which contains the CMV promoter and the Orip/EBNA1 elements and can help obtain the COE plasmid (Figure S1). Plasmids were transformed into competent Escherichia coli DH5α (Tiangen, Beijing, China) for amplification [29]. Then, plasmid extraction was performed using the QIAGEN plasmid extraction kit (QIAGEN, Hilden, Germany).…”