Correlation between parotid saliva composition and dental caries using 31P-NMR and ICDAS score

Rovera, Angela; Rovera, Guido; Alzahrani, Ali S.; Hector, Mark; Anderson, Paul

doi:10.1016/j.archoralbio.2020.104651

Cited by 3 publications

(1 citation statement)

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“…All data were recorded and statistically analyzed using Microsoft Excel 2011 (Microsoft Corporation) and SPSS Statistics (IBM). The sample size was determined based on previous studies (Magnusson et al, 1975 ; Rovera et al, 2020 ). Distribution analysis of data normality was assessed using the Shapiro–Wilk test (normality p > .05).…”

Section: Methodsmentioning

confidence: 99%

Influence of gender and combined estrogen–progestin oral contraceptive on parotid saliva flow rate, pH, and electrolytes concentration

Rovera,

Anderson

2023

Clinical & Exp Dental Res

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ObjectivesEndocrinal variations within an individual impact the electrolyte composition, pH, and flow‐rate (FR) of saliva. The aim of this study was to evaluate the gender‐specific differences and the effect of combined estrogen‐progestin oral contraceptives (COCs) on FR, pH, and electrolyte concentrations in the parotid saliva (PS) of a group of healthy adults.Material and MethodsStimulated PS was collected from 20 healthy adults using a Lashley cup; 11 males, 8 females, and 1 female undertaking combined contraceptive therapy (levonorgestrel/ethinyloestradiol 0.1 mg + 0.02 mg). FR and pH were recorded for each saliva sample. Electrolytes concentrations (Na+, Ca2+, K+, Mg2+) were measured using inductively coupled plasma optical emission spectrometer (ICP‐OES). Statistical analysis was performed, and the significance level was set at p < .05.ResultsPS FR varied from 0.13 to 0.42 mL/min in females not taking any medication and from 0.08 to 0.5 mL/min in males not taking any medication. PS pH of females and males not taking any medication ranged from 6.23 to 7.50 and from 6.15 to 7.55. PS pH and FR of the female taking COCs were 6.5 and 0.1 mL/min. PS pH, FR, and electrolytes concentrations (Ca2+, Na+, K+, Mg2+) were not statistically significantly different between females and males not taking any medication. PS concentrations of Ca2+ and Na+ were significantly higher in the females taking COCs than in the females not taking any medication. Whereas, concentrations of K+ and Mg2+ did not differ significantly between the females taking COCs and the females not taking any medication.ConclusionsThere are no significant gender‐specific differences in PS flow rate, pH, and electrolyte concentrations of Na+, Ca2+, Mg2+, and K+. Combined hormonal oral contraceptive has a significant effect on PS flow rate, pH, Ca2+, and Na+ concentrations. Whereas the PS concentration of K+ and Mg2+ are not influenced by COCs. These results warrant further investigation.

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Section: Methodsmentioning

confidence: 99%

Influence of gender and combined estrogen–progestin oral contraceptive on parotid saliva flow rate, pH, and electrolytes concentration

Rovera,

Anderson

2023

Clinical & Exp Dental Res

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Parotid saliva ¹H‐NMR analysis for colon cancer metabolomics: A case report

Rovera

Hector

Anderson

2021

Special Care in Dentistry

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Background: A key priority in colon cancer research is the identification of molecular biomarkers to improve early diagnosis, guide prognosis, and the design of new therapeutic approaches. Saliva is a powerful diagnostic biofluid that can be used to detect systemic alterations. This study aimed to investigate the parotid saliva (PS) metabolic Proton Nuclear Magnetic Resonance ( 1 H-NMR) profile of a patient diagnosed with colon cancer, and the subsequent changes 1 year after the end of chemotherapy. Case report:We describe the 1 H-NMR PS spectrum of a 65-year-old woman diagnosed with colon cancer (G3 pT3 pN1c) (T0), and the changes in the spectrum from PS collected 1 year after the end of chemotherapy (XELOX: capecitabine plus oxaliplatin) (T1). The data was co-analyzed with blood test cancer antigens (S-CEA; S-CA19-9) and thyroid peroxidase antibody (TPOAb) measurements obtained simultaneously in order to identify peaks and interpret the spectra. The blood cancer antigens (S-CEA; S-CA19-9) and the PS 1 H-NMR peaks for fatty acids, lactate, acetate, N-acetyl sugars, citrate, tyrosine, saccharides, and formate decreased at T1 compared to T0. Whereas, the thyroid peroxidase antibody (TPOAb) blood values increased at T1 compared to T0 reflecting the changes in the 1 H-NMR spectral window of 1-3.5 ppm. Conclusion: PS 1 H-NMR profiling identified modified metabolites that revealed cancer cells metabolism disturbances that subsequently decreased with time throughout treatment. These altered metabolites are potential biomarkers, providing a molecular diagnostic approach for clinical diagnosis, and prognosis of human colon cancer.

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Salivary Redox Biomarkers in the Course of Caries and Periodontal Disease

Skutnik-Radziszewska

Zalewska

2020

Applied Sciences

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Caries are a pathological process of extracorporeal nature, characterized by demineralization of inorganic substances as well as proteolysis triggered by acids produced by bacteria present in dental plaque, as a result of metabolism of sugars of both external and internal origin. Periodontal disease, on the other hand, is a multifactorial degenerative disease associated with inflammation, involving a group of tissues that surround the dental cervix and root of the tooth. It is believed that one of the mechanisms in the etiopathogenesis of caries and periodontitis are disorders of local and/or general oxidative stress (OS) parameters. Numerous clinical studies have confirmed the relationship between oxidative stress markers and oral diseases. In most analyzed studies, technical and biological variability was so high that none of the markers so far has proven suitable for routine clinical use. The aim of systematic reviews of the literature is to present the existing studies on OS parameters, mainly concerning the activity of antioxidant enzymes in saliva of patients with caries and periodontitis.

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Correlation between parotid saliva composition and dental caries using 31P-NMR and ICDAS score

Cited by 3 publications

References 47 publications

Influence of gender and combined estrogen–progestin oral contraceptive on parotid saliva flow rate, pH, and electrolytes concentration

Influence of gender and combined estrogen–progestin oral contraceptive on parotid saliva flow rate, pH, and electrolytes concentration

Parotid saliva ¹H‐NMR analysis for colon cancer metabolomics: A case report

Salivary Redox Biomarkers in the Course of Caries and Periodontal Disease

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Correlation between parotid saliva composition and dental caries using 31P-NMR and ICDAS score

Cited by 3 publications

References 47 publications

Influence of gender and combined estrogen–progestin oral contraceptive on parotid saliva flow rate, pH, and electrolytes concentration

Influence of gender and combined estrogen–progestin oral contraceptive on parotid saliva flow rate, pH, and electrolytes concentration

Parotid saliva 1H‐NMR analysis for colon cancer metabolomics: A case report

Salivary Redox Biomarkers in the Course of Caries and Periodontal Disease

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Product

Resources

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Parotid saliva ¹H‐NMR analysis for colon cancer metabolomics: A case report