Correlation between numbers of cells in human dental pulp and age: Implications for age estimation

Hossain, Mohammad Zakir; Daud, Sulinda; Nambiar, Phrabhakaran; Razak, Fathilah Abdul; Ab‐Murat, Norintan; Saub, Roslan; Bakri, Marina Mohd

doi:10.1016/j.archoralbio.2017.03.018

Cited by 18 publications

(15 citation statements)

References 33 publications

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“…Fibroblasts in the dental pulp are also reported to be involved in pulpitis. Fibroblasts are abundantly present in the dental pulp and are responsible for the synthesis of extracellular matrix and the maintenance of the structural integrity of the dental pulp [6,263,264]. They are also reported to produce pro-inflammatory cytokines, including interleukin (IL)-1β, IL-6 and IL-8, in response to bacterial stimulation [265,266].…”

Section: Involvement Of Trp Channels In the Transduction Of Dentalmentioning

confidence: 99%

The Role of Transient Receptor Potential (TRP) Channels in the Transduction of Dental Pain

Hossain

Bakri

Yahya

et al. 2019

IJMS

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Dental pain is a common health problem that negatively impacts the activities of daily living. Dentine hypersensitivity and pulpitis-associated pain are among the most common types of dental pain. Patients with these conditions feel pain upon exposure of the affected tooth to various external stimuli. However, the molecular mechanisms underlying dental pain, especially the transduction of external stimuli to electrical signals in the nerve, remain unclear. Numerous ion channels and receptors localized in the dental primary afferent neurons (DPAs) and odontoblasts have been implicated in the transduction of dental pain, and functional expression of various polymodal transient receptor potential (TRP) channels has been detected in DPAs and odontoblasts. External stimuli-induced dentinal tubular fluid movement can activate TRP channels on DPAs and odontoblasts. The odontoblasts can in turn activate the DPAs by paracrine signaling through ATP and glutamate release. In pulpitis, inflammatory mediators may sensitize the DPAs. They could also induce post-translational modifications of TRP channels, increase trafficking of these channels to nerve terminals, and increase the sensitivity of these channels to stimuli. Additionally, in caries-induced pulpitis, bacterial products can directly activate TRP channels on DPAs. In this review, we provide an overview of the TRP channels expressed in the various tooth structures, and we discuss their involvement in the development of dental pain.

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Section: Involvement Of Trp Channels In the Transduction Of Dentalmentioning

confidence: 99%

The Role of Transient Receptor Potential (TRP) Channels in the Transduction of Dental Pain

Hossain

Bakri

Yahya

et al. 2019

IJMS

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“…Several methods can be used to estimate chronological age in humans [1][2][3][4][5]. One of the most traditional is the analysis of the degree of mineralization and tooth eruption [1,[6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Tooth wear as an indicator of acculturation process in remote Amazonian populations

et al. 2020

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Riverine populations are typical of the Amazon region that depend on nature for subsistence. These people are considered an intermediate population between the urban and indigenous, the original Amazon habitants. The aim of this cross-sectional study was to evaluate the relationship between tooth wear and age in a remote riverine population from the Amazon, located by the Tucumanduba River (n = 94), and to compare them to previous findings obtained from semi-isolated indigenous (n = 223) and urban populations (n = 40) from the Amazon region, which were examined using the same methodology. Using linear regression, tooth wear explained 54.5% of the variation in the ages of the riverine subjects (p<0.001). This coefficient is midway between those obtained in semi-isolated indigenous populations (65-86%) and urban subjects (12%) living in the Amazon. Our findings suggest that tooth wear, a direct evidence of what an individual ate in the past, may be an indicator of the acculturation process in remote populations.

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“…At the time the tooth erupts and reaches the occlusal plane, most organelles of the mature odontoblasts relocate to the distal pole and autophagy vacuoles start to accumulate. Hence, the secretion of secondary dentin is significantly slower (0.5 μm per day), and also the number of cells decreases . Maturing odontoblasts gradually flatten and finally form the typical monolayer at the pulp‐dentin‐interface .…”

Section: Introductionmentioning

confidence: 99%

Isolation of primary odontoblasts: Expectations and limitations

et al. 2019

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The purpose of this study was to evaluate different protocols of enzymatic treatment (collagenase with either protease, trypsin or hyaluronidase) to isolate mature odontoblasts. Primary odontoblasts were obtained from human molars, which was confirmed by histology and scanning electron microscopy. The combination of collagenase with protease appeared most suitable and resulted in higher cell numbers and better integrity of the odontoblast processes, whereas combination with hyaluronidase or trypsin led to truncated processes and detachment of cell patches instead of single cells. However, trypan blue staining after 24 h showed that odontoblasts in culture did not remain viable. Gene expression analysis was possible after mRNA extraction from tissues ex vivo and real-time semi-quantitative PCR revealed increased expression of collagen, nestin, bone sialoprotein and dentin matrix acidic phosphoprotein 1 in the odontoblast layer. Though primary odontoblasts could not be cultivated after isolation, characteristic genes were identified to differentiate odontoblasts from pulp fibroblasts.

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Correlation between numbers of cells in human dental pulp and age: Implications for age estimation

Abstract: A significant correlation between the cell count of odontoblasts and subodontoblasts with age was demonstrated. Regression equations using odontoblasts and subodontoblasts cell number can be used to predict age with some limitations.

Cited by 18 publications

References 33 publications

The Role of Transient Receptor Potential (TRP) Channels in the Transduction of Dental Pain

The Role of Transient Receptor Potential (TRP) Channels in the Transduction of Dental Pain

Tooth wear as an indicator of acculturation process in remote Amazonian populations

Isolation of primary odontoblasts: Expectations and limitations

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