Correlation between KLOTHO gene and mild cognitive impairment in the Uygur and Han populations of Xinjiang

Abstract

The secretive Klotho protein is an anti-aging regulatory hormone that plays a physiological role in many target organs. The present study aims to investigate the correlation between Klotho gene and mild cognitive impairment (MCI) in Uygur and Han populations in Xinjiang. From July 2008 to April 2014, stratified random multistage cluster sampling was used in combination with the methods of on-site questionnaire and household survey to conduct a cross-sectional MCI investigation on selected Uygur and Han subjects aged over 60 years old in Xinjiang region. 323 Uygur and Han MCI patients were randomly selected and matched with 343 subjects in the normal control group. SNaPshot technique was used to detect the polymorphisms of Klotho gene. A case-control associated analysis was conducted to analyze the genotype and allele frequencies of single nucleotide polymorphisms (SNPs) in the MCI group and the normal control group. The polymorphisms of rs1207568 and rs9536314/rs9527025 loci in Klotho gene were different among MCI populations in Xinjiang, and after group assignments based on ethnic background, the polymorphisms of rs1207568 and rs9536314/rs9527025 loci were associated with the Uygur MCI population and were not relevant to the Han MIC population. The frequencies of mutational alleles of rs9536314/rs9527025 locus in the Uygur population were significantly higher than those in the Han population. The genotype and allele frequencies of rs1207568 locus in the Uygur and Han populations were similar. The polymorphisms of rs1207568 and rs9536314/rs9527025 loci in Klotho gene may be associated with the Uygur MCI population in Xinjiang.

Nitride oxide synthase 3 and klotho gene polymorphisms in the pathogenesis of chronic kidney disease and age-related cognitive impairment: a systematic review and meta-analysis

“…Our final paper selection identified 21 papers 22 – 42 assessing NOS3 G894T gene polymorphisms in age-related cognitive impairment; three papers 22 , 43 , 44 assessing NOS3 T786C gene polymorphisms in age-related cognitive impairment; five papers 45 – 49 assessing KL G395A gene polymorphisms in age-related cognitive impairment; ten papers 50 – 59 evaluating NOS3 4b/4a gene polymorphisms in CKD; seven papers 55 – 57 , 59 – 62 evaluating NOS3 G894T gene polymorphisms in CKD; three papers 50 , 55 , 63 assessing NOS3 T786C gene polymorphisms in CKD; six papers 64 – 69 assessing KL G395A gene polymorphisms in CKD; and three papers 66 – 68 assessing NOS3 C1818T gene polymorphisms in CKD. This number of papers were searched in PubMed, Embase, Cochrane, and Web of Science; and papers were selected in accordance with inclusion and exclusion criteria.…”

Association of Genetic Variants of <b><i>Klotho</i></b> with BP Responses to Dietary Sodium or Potassium Intervention and Long-Term BP Progression

“…According to previous studies, rs9527026 and rs953614 were located at a perfect LD haplotype termed KL-VS [15]. This haplotype was present in approximately 15–18% of American, European, and Chinese Uygur populations [16]. However, in the Chinese Han population, the MAF was <5%.…”

Klotho and the Treatment of Human Malignancies

“…Klotho expression has been shown to be significantly down-regulated in malignant tissue compared to adjacent non-malignant tissue using immunohistochemical (IHC) techniques, including colorectal [44,45], pancreatic [46], gastric [47], oesophageal [48], breast [49], hepatocellular [50], ovarian [51], and renal cancers [52] (Table 1). Furthermore, more than ten single nucleotide polymorphisms (SNPs) have been identified in the human KLOTHO gene, and a number of studies have been performed to evaluate the associations between allelic variations in the KLOTHO gene and the aetiology of ageing-related diseases [53][54][55][56][57][58][59][60][61], including the effect of Klotho's functional variants on cancer predisposition [62][63][64]. A meta-analysis of 29 case-control studies has been undertaken to derive a pooled estimate of effect and to quantify the variability observed between individual studies [65].…”