Correlation between follicular fluid levels of sRAGE and vitamin D in women with PCOS

Garg, Deepika; Grazi, Richard V.; Lambert‐Messerlian, Geralyn; Merhi, Zaher

doi:10.1007/s10815-017-1011-6

Cited by 28 publications

(12 citation statements)

References 45 publications

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“…Our previous study found that the follicular fluid sRAGE has a positive correlation with ovarian reservation and decreased in PCOS compared with women without PCOS [7] . One research supported our point that sRAGE could represent a biomarker and a potential therapeutic target for ovarian dysfunction in PCOS [8] .…”

Section: Introductionsupporting

confidence: 52%

sRAGE downregulates the VEGF expression through EGF-like growth factor signal pathway in PCOS ovarian granulosa cells

Wang

Guan

Yi³

et al. 2020

Preprint

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Objective High expression of VEGF in ovarian tissue, serum and follicular fluid of PCOS women is involved in the physiological and pathogenesis processes of PCOS. Our objective was to investigate the effect of sRAGE on VEGF expression and EGF-like growth factor in PCOS ovarian granulosa cells. Methods We collected ovarian granulosa cells of PCOS patients who underwent in vitro fertilization (IVF). Then treatment ovarian granulosa cells with different concentrations of sRAGE. Levels of VEGF, AREG, BTC and EREG mRNA were examined by quantitative RT-PCR. The protein levels of VEGF, AREG, BTC and EREG were measured by ELISA. Results Treatment with sRAGE decrease the production of VEGF, and the effects were dependent on the concentrations of sRAGE ( P <0.05). Simultaneously, the expression of the EGF-like growth factors AREG, BTC and EREG were decreased, and the expression were dependent on the concentrations of sRAGE ( P <0.05). Conclusions sRAGE may downregulate VEGF expression via EGF-like growth factor pathway in PCOS ovarian granulosa cells.

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Section: Introductionsupporting

confidence: 52%

sRAGE downregulates the VEGF expression through EGF-like growth factor signal pathway in PCOS ovarian granulosa cells

Wang

Guan

Yi³

et al. 2020

Preprint

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“…Moreover, serum AGEs correlate positively with altered glucose metabolism, age and factors related to obesity, dyslipidaemia, hyperglycaemia and insulin resistance in patients undergoing in vitro fertilization (IVF) [14]. Further evidence for the role of AGEs in PCOS was provided by measurements in granulosa cells and serum of soluble receptors for AGEs (s-RAGE), a circulating isoform of RAGE that can neutralize the ligand-mediated damage [15]. A potential role of MG in PCOS was supported by the finding that dietary glycotoxins and hyperandrogenic status decrease GLO1 activity in rat ovaries, possibly contributing to increased AGE accumulation in granulosa cells [16].…”

Section: Introductionmentioning

confidence: 99%

Methylglyoxal-Dependent Glycative Stress and Deregulation of SIRT1 Functional Network in the Ovary of PCOS Mice

Emidio

Placidi

Rea

et al. 2020

Cells

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Advanced glycation end-products (AGEs) are involved in the pathogenesis and consequences of polycystic ovary syndrome (PCOS), a complex metabolic disorder associated with female infertility. The most powerful AGE precursor is methylglyoxal (MG), a byproduct of glycolysis, that is detoxified by the glyoxalase system. By using a PCOS mouse model induced by administration of dehydroepiandrosterone (DHEA), we investigated whether MG-dependent glycative stress contributes to ovarian PCOS phenotype and explored changes in the Sirtuin 1 (SIRT1) functional network regulating mitochondrial functions and cell survival. In addition to anovulation and reduced oocyte quality, DHEA ovaries revealed altered collagen deposition, increased vascularization, lipid droplets accumulation and altered steroidogenesis. Here we observed increased intraovarian MG-AGE levels in association with enhanced expression of receptor for AGEs (RAGEs) and deregulation of the glyoxalase system, hallmarks of glycative stress. Moreover, DHEA mice exhibited enhanced ovarian expression of SIRT1 along with increased protein levels of SIRT3 and superoxide dismutase 2 (SOD2), and decreased peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC1α), mitochondrial transcriptional factor A (mtTFA) and translocase of outer mitochondrial membrane 20 (TOMM20). Finally, the presence of autophagy protein markers and increased AMP-activated protein kinase (AMPK) suggested the involvement of SIRT1/AMPK axis in autophagy activation. Overall, present findings demonstrate that MG-dependent glycative stress is involved in ovarian dysfunctions associated to PCOS and support the hypothesis of a SIRT1-dependent adaptive response.

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“…Our previous study found that the follicular uid sRAGE has a positive correlation with ovarian reservation and decreased in PCOS compared with women without PCOS [7] . One research supported our point that sRAGE could represent as a biomarker and a potential therapeutic target for ovarian dysfunction in PCOS [8] . Interestingly, we have found that follicular uid sRAGE levels were negatively correlated with VEGF in PCOS [9] .…”

Section: Introductionmentioning

confidence: 52%

sRAGE Downregulates the VEGF Expression in PCOS Ovarian Granulosa Cells

Wang

Guan

Liu³

et al. 2020

Preprint

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Objective High expression of VEGF in ovarian tissue, serum and follicular fluid of PCOS women is involved in the physiological and pathogenesis processes of PCOS. Our objective was to investigate the effect of sRAGE on VEGF expression and EGF-like growth factor in PCOS ovarian granulosa cells.Methods We collected ovarian granulosa cells of PCOS patients who underwent in vitro fertilization (IVF). Then treatment ovarian granulosa cells with different concentrations of sRAGE. Levels of VEGF, AREG, BTC and EREG mRNA were examined by quantitative RT-PCR. The protein levels of VEGF, AREG, BTC and EREG were measured by ELISA.Results Treatment with sRAGE decrease the production of VEGF, and the effects were dependent on the concentrations of sRAGE (P < 0.05). Simultaneously, the expression of the EGF-like growth factors AREG, BTC and EREG were decreased, and the expression were dependent on the concentrations of sRAGE (P < 0.05).Conclusions sRAGE may downregulate VEGF expression in PCOS ovarian granulosa cells,and EGF-like growth factor pathway may be involved in this process.

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Correlation between follicular fluid levels of sRAGE and vitamin D in women with PCOS

Cited by 28 publications

References 45 publications

sRAGE downregulates the VEGF expression through EGF-like growth factor signal pathway in PCOS ovarian granulosa cells

sRAGE downregulates the VEGF expression through EGF-like growth factor signal pathway in PCOS ovarian granulosa cells

Methylglyoxal-Dependent Glycative Stress and Deregulation of SIRT1 Functional Network in the Ovary of PCOS Mice

sRAGE Downregulates the VEGF Expression in PCOS Ovarian Granulosa Cells

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