sRAGE downregulates the VEGF expression through EGF-like growth factor signal pathway in PCOS ovarian granulosa cells

Abstract

Objective High expression of VEGF in ovarian tissue, serum and follicular fluid of PCOS women is involved in the physiological and pathogenesis processes of PCOS. Our objective was to investigate the effect of sRAGE on VEGF expression and EGF-like growth factor in PCOS ovarian granulosa cells. Methods We collected ovarian granulosa cells of PCOS patients who underwent in vitro fertilization (IVF). Then treatment ovarian granulosa cells with different concentrations of sRAGE. Levels of VEGF, AREG, BTC and EREG mRNA were examined by quantitative RT-PCR. The protein levels of VEGF, AREG, BTC and EREG were measured by ELISA. Results Treatment with sRAGE decrease the production of VEGF, and the effects were dependent on the concentrations of sRAGE ( P <0.05). Simultaneously, the expression of the EGF-like growth factors AREG, BTC and EREG were decreased, and the expression were dependent on the concentrations of sRAGE ( P <0.05). Conclusions sRAGE may downregulate VEGF expression via EGF-like growth factor pathway in PCOS ovarian granulosa cells.

Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos

“…Previous studies were compatible with our findings that higher concentrations of EGF, 40 or 50 ng/mL, reduced the blastocyst development rather than the 20 ng/mL, which may be attributed to a suggested phenomenon of growth factor-induced receptor down-regulation. Generally, EGF was previously reported to enhance the cellular function causing early embryo development in mammals; more specifically, our study elucidated that the optimum way to add EGF only as a supplement to in-vitro-produced buffaloes' embryos was 20 ng/mL added to the TCM-199 media (14).…”

“…The aspirated oocytes with compact multi-layered cumulus cells and evenly granulated cytoplasm were selected for IVM. The recovered COCs were classified according to their quality into; good oocytes with homogenous granular ooplasm surrounded by more than three-compact layers of cumulus cells, fair oocytes with homogenous ooplasm surrounded by 1-3 layers of cumulus cells, and denuded oocytes with uneven ooplasm (14).…”

“…Also, Adding EGF alone to IVM media increases the cumulus cell expansion and enhances the nuclear maturation of bovine oocytes. For oocyte maturation and embryo development, antioxidants play a pivotal role in many mammalian species (14) because excessive production of reactive oxygen species negatively affects oocyte maturation and embryo development (15).…”