Correlation between fertility and levels of protein, sugar and free amino acids in seminal plasma of Nelore bulls

Assumpção, Teresinha Inês de; Júnior, R.A.A. Torres; Sousa, Marcelo Valle de; Ricart, Carlos André Ornelas

doi:10.1590/s0102-09352005000100008

Cited by 19 publications

(15 citation statements)

References 18 publications

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“…When these amino acids in seminal plasma were compared to those found in human seminal plasma (Li et al, 2019), profiles of some seminal plasma amino acids were similar to those profiles we found such as serine, glycine, and glutamic acids. The least abundant in our study, on the other hand, are tyrosine, methionine, alphaaminobutyric acid, allo-isoleucine, and asparagine, and are found to be similar with the low levels of amino acids of methionine and tyrosine in bull (Assumpção et al, 2005). Also, in domestic fowl, valine, serine, glycine, and alanine were the most abundant amino acids followed by glutamic acid (Santiago-Moreno et al, 2019).…”

Section: Discussionsupporting

confidence: 73%

Amino Acids of Seminal Plasma Associated With Freezability of Bull Sperm

Ugur

Hitit

Kaya

et al. 2020

Front. Cell Dev. Biol.

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Sperm cryopreservation is an important technique for fertility management, but postthaw viability of sperm differs among breeding bulls. With metabolites being the end products of various metabolic pathways, the contributions of seminal plasma metabolites to sperm cryopreservation are still unknown. These gaps in the knowledge base are concerning because they prevent advances in the fundamental science of cryobiology and improvement of bull fertility. The objective of this study was to test the hypothesis that seminal plasma amino acids are associated with freezability of bull sperm. To accomplish this objective, amino acid concentrations in seminal plasma from seven bulls of good freezability (GF) and six bulls of poor freezability (PF) were quantified using gas chromatography-mass spectrometry (GC-MS). Multivariate and univariate analyses were performed to identify potential freezability biomarkers. Pathways and networks analyses of identified amino acids were performed using bioinformatic tools. By analyzing and interpreting the results we demonstrated that glutamic acid was the most abundant amino acid in bull seminal plasma with average concentration of 3,366 ± 547.3 nM, which accounts for about 53% of total amino acids. The other most predominant amino acids were alanine, glycine, and aspartic acid with the mean concentrations of 1,053 ± 187.9, 429.8 ± 57.94, and 427 ± 101.3 nM. Pearson's correlation analysis suggested that phenylalanine concentration was significantly associated with post-thaw viability (r = 0.57, P-value = 0.043). Significant correlations were also found among other amino acids. In addition, partial least squares-discriminant analysis (PLS-DA) bi-plot indicated a distinct separation between GF and PF groups. Phenylalanine had the highest VIP score and was more abundant in the GF groups than in the PF groups. Moreover, pathway and network analysis indicated that phenylalanine contributes to oxidoreductase and antioxidant reactions. Although univariate analysis did not yield significant differences in amino acid concentration between the two groups, these findings are significant that they indicate the potentially important roles of amino acids in seminal plasma, thereby building a foundation for the fundamental science of cryobiology and reproductive biotechnology.

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Section: Discussionsupporting

confidence: 73%

Amino Acids of Seminal Plasma Associated With Freezability of Bull Sperm

Ugur

Hitit

Kaya

et al. 2020

Front. Cell Dev. Biol.

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“…A positive correlation has been observed between Mg and apoptosis‐free viable cells in rams (Juyena, 2011). Assumpção et al (2005) observed a negative correlation of abnormal sperm morphology with P, Ca, and Na concentrations and a positive correlation with K concentration in bull semen. Cu is necessary for many enzymes, like Cu‐Zn‐SOD, which is involved in cell protection against free radicals.…”

Section: Biochemical Constituents Of Sp and Their Functionsmentioning

confidence: 98%

Seminal Plasma: An Essential Attribute to Spermatozoa

Juyena¹,

Stelletta²

2012

Journal of Andrology

221

169

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The biochemical composition of seminal plasma (SP) is very complex and variable among species. Advances in reproductive technologies reveal SP as a nutritive-protective medium for sperm cells suspended in it, and some SP components are very important for sperm metabolism, as well as sperm function, survival, and transport in the female reproductive tract. Biochemistry of SP is a relatively modern but rapidly expanding field of research, particularly concerning the biological significance of the various biochemical constituents of SP. However, identification of active factors in SP and mechanisms by which they act are not sufficiently understood in different species. Especially in camelids, the origin, composition, and function of the viscous component of SP remain a mystery, and only some biochemical and morphological characteristics of SP have been described. In this review, we focus on the current understanding of physiological and biochemical properties of SP in ruminants and camelids.

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“…The composition of SP shows high individual variability in Nellore bulls [10], a fact that results in different responses of the animals to sperm cryopreservation [11,12]. However, the main components of SP (on a weight basis) are proteins, which are considered the predominant modulator of sperm function [13].…”

Section: Introductionmentioning

confidence: 99%

Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility

et al. 2017

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Cryopreservation of bull semen is a common biotechnology procedure in cattle breeding. However, when the ejaculate is obtained by electroejaculation, wide variation is observed in the sperm/seminal plasma (SP) ratio that can affect the freezability of semen in this species. The removal of SP may improve the quality of frozen bull semen. The objective of this study was to evaluate the effect of SP removal from the ejaculate on the cryopreservation of semen from 38 Nellore bulls collected by electroejaculation. After collection, the ejaculate was divided into three aliquots: (1) control (N) diluted to a concentration of 60 × 10 spermatozoa/mL and frozen with SP; (2) centrifugation (C) at ×600g for 10 minutes and the pellet resuspended and frozen at the same concentration as N; and (3) filtration (F) through SpermFilter and sperm recovered and frozen at the same concentration as N. After thawing, sperm kinetics, plasma and acrosome membrane integrity, mitochondrial membrane potential, oxidative stress, and in vitro fertility were evaluated. Statistical analysis was performed using the SAS 9.2 package, and differences were considered significant when P < 0.05. Higher average path velocity and straight-line velocity were observed in the groups submitted to SP removal compared to the control group (P < 0.01). In contrast, filtered samples exhibited higher beat cross frequency, straightness, and linearity compared to the other groups. Plasma membrane integrity was reduced when SP was removed, but lower oxidative stress was observed in groups C and F (34.91 ± 2.95% and 31.63 ± 2.95%, respectively) compared to group N (57.39 ± 2.95%). However, the percentage of hatched blastocysts was similar in the N and F groups (21.22 ± 1.05% and 24.00 ± 1.05%, respectively) and higher compared to group C (18.83 ± 1.05%). In conclusion, removal of SP by centrifugation for bull semen freezing reduced the rate of in vitro-produced embryos, whereas filtration of prefrozen semen was found to be an efficient alternative in terms of semen freezability and in vitro production of bovine embryos.

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Correlation between fertility and levels of protein, sugar and free amino acids in seminal plasma of Nelore bulls

Cited by 19 publications

References 18 publications

Amino Acids of Seminal Plasma Associated With Freezability of Bull Sperm

Amino Acids of Seminal Plasma Associated With Freezability of Bull Sperm

Seminal Plasma: An Essential Attribute to Spermatozoa

Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility

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