Correlation between enzymes inhibitory effects and antioxidant activities of standardized fractions of methanolic extract obtained from Ficus deltoidea leaves

Farsi, Elham; Shafaei, Armaghan; Hor, Sook Yee; Ahamed, Mohamed B. Khadeer; Yam, Mun Fei; Attitalla, Idress Hamad; Asmawi, Mohd Zaini; Ismail, Zhari

doi:10.5897/ajb11.1365

Cited by 29 publications

(23 citation statements)

References 28 publications

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“…The · OH scavenging activity had a moderate correlation with α-glucosidase inhibitory activity (R 2 = 0.4705), a strong correlation with α-amylase inhibitory activity (R 2 = 0.8967), and a poor correlation with pancreatic lipase inhibitory activity (R 2 = 0.1140). These findings therefore revealed that the flavonoid with higher · OH scavenging activity exhibited higher α-glucosidase and α-amylase inhibitory activities, which was in agreement with the result reported by Farsi et al (2011).…”

Section: Inhibitory Activity and Kinetic Analysis Of Pancreatic Lipasesupporting

confidence: 82%

In vitro antioxidant, anti-diabetic and antilipemic potentials of quercetagetin extracted from marigold (Tagetes erecta L.) inflorescence residues

Wang

Chen

et al. 2016

J Food Sci Technol

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Quercetagetin, the major flavonoid in marigold (Tagetes erecta L.) inflorescence residues was extracted and purified. The content of quercetagetin after the purification was 89.91 ± 0.26 %. The in vitro antioxidant activity of quercetagetin and its potential in controlling diabetes mellitus and obesity were investigated and compared to quercetin and rutin. The 50 % inhibitory concentration (IC 50 ) values of quercetagetin on scavenging 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) and hydroxyl radicals were 27.12 ± 1.31 μmol/L, 12.16 ± 0.56 μmol/L and 1833.97 ± 6.66 μmol/L, respectively. The IC 50 values of quercetagetin on α-glucosidase, α-amylase and pancreatic lipase were 180.11 ± 3.68 μmol/L, 137.71 ± 3.55 μmol/L and 2327.58 ± 12.37 μmol/L, respectively. These results indicated that quercetagetin exhibited strong in vitro antioxidant, anti-diabetic and antilipemic activities. Lineweaver-Burk plots analysis elucidated that quercetagetin inhibited α-glucosidase and α-amylase non-competitively, while its inhibition against pancreatic lipase was involved in a mixed-type pattern. Moreover, strong correlations were found between ABTS ·+ /DPPH · scavenging activities and lipase inhibitory activity (R 2 > 0.90), as well as ·OH scavenging activity and α-amylase inhibitory activity (R 2 = 0.8967).

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Section: Inhibitory Activity and Kinetic Analysis Of Pancreatic Lipasesupporting

confidence: 82%

In vitro antioxidant, anti-diabetic and antilipemic potentials of quercetagetin extracted from marigold (Tagetes erecta L.) inflorescence residues

Wang

Chen

et al. 2016

J Food Sci Technol

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“…However, the recent studies correlated the remarkable antioxidant and α-glucosidases inhibitory activities of F. deltoidea with its antidiabetic property [10,15]. As circumstance this study aims to investigate the possible mechanism responsible for glucose lowering effect of F. deltoidea by studying the expression of GK, Glc-6-Pase, PEPCK, GLUT2 and PPARγ genes in liver and GLUT4 in muscle of streptozitocin-induced diabetic rats.…”

Section: Introductionmentioning

confidence: 99%

Standardized extract of Ficus deltoidea stimulates insulin secretion and blocks hepatic glucose production by regulating the expression of glucose-metabolic genes in streptozitocin-induced diabetic rats

Farsi

Ahmad

Hor

et al. 2014

BMC Complement Altern Med

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BackgroundRecently, there has been increasing interest in Ficus deltoidea Jack. (Moraceae) due to its chemical composition and the potential health benefits. The present study was undertaken to investigate the effect of extracts of F. deltoidea leaves on diabetes.MethodsThe petroleum ether, chloroform and methanol extracts of F. deltoidea were prepared and subjected to standardization using preliminary phytochemical and HPLC analysis. Dose selection was made on the basis of acute oral toxicity study (50–5000 mg/kg b. w.) as per OECD guidelines. Diabetes mellitus was induced with streptozotocin and rats found diabetic were orally administered with the extract (250, 500 and 1000 mg/kg) for 14 days. Levels of blood glucose and insulin were measured in control as well as diabetic rats on 0, 7 and 14th day. In addition, glucose metabolism regulating gene expression was assessed using RT-PCR.ResultsHPLC analysis revealed that the methanol extract is enriched with C-glycosylflavones particularly, vitexin and isovitexin. In oral glucose tolerance test, oral administration of the methanol extract increased the glucose tolerance. The methanol extract showed significant (P < 0.01) antidiabetic activity. The extract treatment caused significant reduction (p < 0.01) in elevated fasting blood glucose level in streptozotocin-induced diabetic rats. The streptozotocin-related weight loss in rats was noticeably reversed by the extract treatment. Finally, RT-PCR analysis revealed a novel mechanisms for the anti-diabetic action of methanol extract of F. deltoidea. The extract exerted its effect via an increase of insulin secretion which impeded the hepatic glucose production, via down-regulation of phosphoenolpyruvate carboxykinase and glucose-6-phosphatase genes expression on one hand, and up-regulation of hepatic GK and PPARγ genes expression on the other hand. The extract caused an increased expression of GLUT-4 gene expression in skeletal muscles which leads to normalize the hyperglycemia. The extract also nullified the toxic effects of streptozitocin by blocking its entry into the islet β-cells through reducing the expression of GLUT-2 gene.ConclusionIt can be concluded that, F. deltoidea could potentially inhibits the streptozitocin-induced hyperglycemia in rats. Further the herb can be utilized as useful remedy for alleviation of diabetes complications.

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“…The leaf extracts were reported to be rich of phenolic and flavonoid compounds which are comparable with black and green teas as well as fruit juices [11]. The flavan-3-ols and flavones were the main compounds that contributed to the total antioxidant activity [11], whereas isovetexin and vetexin were reported to be responsible for the antidiabetic activity [14].…”

Section: Introductionmentioning

confidence: 99%

Morphological Phylogenetic Analysis of Seven Varieties of Ficus deltoidea Jack from the Malay Peninsula of Malaysia

et al. 2012

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This study is the first report to suggest a morphological phylogenetic framework for the seven varieties of Ficus deltoidea Jack (Ficus: Moraceae) from the Malay Peninsula of Malaysia. Several molecular-based classifications on the genus Ficus had been proposed, but neither had discussed the relationship between seven varieties of F. deltoidea to its allies nor within the varieties. The relationship between seven varieties of F. deltoidea is still debated due to the extreme morphological variabilities and ambiguous boundaries between taxa. Thus, the correct identification of these varieties is important as several morphological characters are variety-specific. To test the monophyly and further resolved the relationship in F. deltoidea, a morphological phylogenetic analysis was conducted based on herbarium specimens representing the seven varieties of F. deltoidea that were collected from the Malay Peninsula of Malaysia, by using related species of the genus Ficus; F. grossularioides, F. ischnopoda and F. oleifolia as the outgroups. Parsimony and neighbour-joining analyses indicated that F. deltoidea is monophyletic, in that the seven varieties of F. deltoidea nested into two clades; clade subspecies deltoidea (var. deltoidea, var. bilobata, var. angustifolia, var. kunstleri and var. trengganuensis) and clade subspecies motleyana (var. intermedia and var. motleyana).

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Correlation between enzymes inhibitory effects and antioxidant activities of standardized fractions of methanolic extract obtained from Ficus deltoidea leaves

Cited by 29 publications

References 28 publications

In vitro antioxidant, anti-diabetic and antilipemic potentials of quercetagetin extracted from marigold (Tagetes erecta L.) inflorescence residues

In vitro antioxidant, anti-diabetic and antilipemic potentials of quercetagetin extracted from marigold (Tagetes erecta L.) inflorescence residues

Standardized extract of Ficus deltoidea stimulates insulin secretion and blocks hepatic glucose production by regulating the expression of glucose-metabolic genes in streptozitocin-induced diabetic rats

Morphological Phylogenetic Analysis of Seven Varieties of Ficus deltoidea Jack from the Malay Peninsula of Malaysia

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