Correlation between CT features of adrenocortical and adrenal medullary tumors and expression of miR‑96 in serum

Abstract

Correlation between CT features of adrenocortical and adrenal medullary tumors and the expression of miR-96 in serum were investigated. A total of 230 patients with adrenocortical tumors and 194 patients with adrenal medullary tumors were selected in Dongying People's Hospital from August 2013 to August 2017. The two groups of patients underwent CT examination, and the signs and symptoms were recorded. The expression of miR-96 in the serum of the two groups was detected by RT-PCR, and the correlation between the expression of serum miR-96 and CT features was analyzed. In patients with adrenocortical tumor, serum miR-96 expression levels were significantly higher in patients with tumor diameter ≥5 cm than those with tumor diameter <5 cm (p<0.001). In patients with adrenal medullary tumor, serum miR-96 expression levels were significantly higher in patients with tumor diameter ≥3 cm than those with tumor diameter <3 cm (p<0.001). In patients with adrenocortical or adrenal medullary tumor, serum miR-96 expression levels were significantly higher in patients with peripheral infiltration than those without peripheral infiltration (p<0.001), and serum miR-96 expression levels were also significantly higher in patients with distant metastasis than those without distant metastasis (p<0.001). Serum levels of miR-96 in patients with benign adrenocortical and adrenal medullary tumors were significantly lower than those with malignant tumors in the same group (p<0.001). miR-96 may have oncogenic functions in patients with adrenocortical or adrenal medullary tumors. Increased expression level of miR-96 may promote proliferation, invasion and metastasis of tumors, and serum levels of miR-96 provide references for the diagnosis of adrenocortical and adrenal medullary tumors.

miR-96-5p promotes the proliferation and migration of ovarian cancer cells by suppressing Caveolae1

“…In addition, we found a positive correlation of miR-96-5p between OC tissues and serum samples. miR-96-5pIn fact, miR-96-5p has shown increased levels in serum samples from patients with breast cancer [8], prostate cancer [26], hepatocellular carcinoma [27], adrenocortical and adrenal medullary tumors [11] compared to healthy controls, and its level is closely related to tumor size, malignancy and metastasis. Our study revealed for the first time the expression pattern of miR-96-5p in OC tissues and cells, and in serum samples from OC patients, which were consistent with its expression patterns in other types of tumors.…”

“…Among miRNAs, microRNA-96 (miR-96-5p) is one member of the miR-183-96-182 cluster which serves an important role in the regulation of the cancer cells’ biological behavior. MiR-96-5p has been found to be highly up-regulated in different kind of tumors, including breast cancer [8], thyroid cancer [9], bladder cancer [10], adrenocortical and adrenal medullary tumors [11], head and neck squamous cell carcinoma, and be significant down-regulated in osteosarcoma [12]. MiR-96-5p has been demonstrated to be related with the development and progression of these cancers.…”