Correlation between cell-free mRNA expressions and PLGF protein level in severe preeclampsia

Abstract

BackgroundPreeclampsia is a major cause of morbidity and mortality, both maternal and perinatal. The etiology and pathophysiology of preeclampsia remain unknown. Research shows the implantation of the placenta in preeclampsia occurs due to incomplete angiogenic imbalance as one of the preeclampsia pathogenesis. PlGF is angiogenic protein which is synthesized in placenta by mRNA PlGF. When damage occurs, mRNA will be released from cell and form cell-free mRNA. This study aims to analyze the differences between the PlGF mRNA expression in severe preeclampsia and normal pregnancy as well as to measure the relationship between cell-free mRNA and levels of PlGF with the incidence of severe preeclampsia.MethodsThe method used in this study is an observational analytic study with cross-sectional design. Blood samples were obtained from patients with preeclampsia and normal pregnancies as the controlling factors in accordance with inclusion and exclusion criterias. Examination of the PlGF level was measured by ELISA method and mRNA PIGF expression was measured by RT-PCR. Physical and laboratory examinations of patients were recorded and collected as data. Calculations were done by statistical analysis.ResultsMean of the cell-free mRNA PlGF expression level in severe preeclampsia is 2.2983 ng/mL within the scale of 1.96–2.83 ng/mL and deviation standard of 0.1897. Using Pearson Analysis Test, the result shows that there is a positive correlation between cell-free mRNA expression and PlGF protein level in severe preeclampsia, with r = 0.640 dan p < 0.004.ConclusionThere is no difference between expression of cell-free mRNA PlGF in severe preeclampsia serum and normal pregnancy. There is a significant correlation between expression of cell-free mRNA and PlGF protein level in severe preeclampsia.

Expression of placental growth factor mRNA in preeclampsia

“…Only a few studies reported mRNA alterations in patients affected to PE ( 12 , 29 , 32 ). Our results are in accordance with the findings of previous studies which reported no significant difference between the level of PlGF mRNA expression in PE women compared to normal controls ( 8 , 30 , 31 ). Tsatsaris and coworkers studied about dysregulation in the VEGF family in PE and reported no difference in PlGF mRNAs expression among the early onset severe PE patients and healthy controls ( 8 ).…”

“…Also Pramatirta and coworkers reported no difference between expression of cell-free mRNA of PlGF between serum of severe preeclamptic women and those with normal pregnancy (31). …”

“…TOFT and colleagues worked on expression of genes which regulate angiogenesis in PE including PlGF in placentas from preeclamptic women by whole-genome microarray and reported no difference between this group and controls ( 30 ). Also Pramatirta and coworkers reported no difference between expression of cell-free mRNA of PlGF between serum of severe preeclamptic women and those with normal pregnancy ( 31 ).…”

G-protein subunit α-14 is dysregulated in human placentas from systemic lupus erythematosus pregnancies

“…Studies investigating the correlations between mRNA and protein abundance in human tissues are controversial [21][22][23]. A study involving 23 human cell lines showed that correlations were only detected in one-third of the mRNA and corresponding proteins [24].…”