Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates

Brolazo, Eliane Melo; Leite, Domingos S.; Tiba, Monique Ribeiro; Villarroel, Marina; Marconi, Camila; Simões, J. A.

doi:10.1590/s1517-83822011000100028

Cited by 22 publications

(19 citation statements)

References 20 publications

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“…lactis with an identity level of 73.5%, and was contrary to the BLAST analysis, which showed homology to the genus Weissella . Previous research has also shown a difference between biochemical test results using API 50 CHL and molecular analyses [19]; we have also found contradicting results when comparing the API kit to molecular technique. While in this study, the results of molecular analysis were Lactobacillus crispatus (32.6%), Lactobacillus jensenii (25%), and Lactobacillus gasseri (20.6%), the results of the API 50 CHL Kit analysis found Lactobacillus acidophilus (34.8%), L. crispatus (27.2%), and Lactobacillus fermentum (13%).…”

Section: Discussioncontrasting

confidence: 91%

Weissella, a novel lactic acid bacteria isolated from wild Sumatran orangutans (Pongo abelii)

et al. 2019

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Aim: This study aimed to isolate and identify lactic acid bacteria (LAB) in wild Sumatran orangutans to provide more information about LAB diversity derived from Sumatran orangutan feces. Materials and Methods: Fecal sampling from three female orangutans, around 35 years old, was carried out in the wild forest areas at the research station of Suaq Belimbing Gunung Leuser National Park located in the South Aceh district. Orangutan fecal samples were taken in the morning when the orangutans first defecated. The orangutans were above the tree, which is approximately 12-15 m from the ground where feces were found. Results: Fermentation testing using the API 50 CHL Kit showed that OUL4 isolates were identified as Lactococcus lactis ssp. lactis with an identity value of 73.5%. Homology analysis demonstrated that the OUL4 isolates have 93% similarity to Weissella cibaria, and phylogenetic trees constructed using Mega 7.0 also showed that OUL4 isolates are related to W. cibaria. Conclusion: These results show that there is a difference in identification between biochemical testing with API kits and molecular analyses on LAB isolates from wild Sumatran orangutans. Based on 16S rRNA gene homology, the OUL4 LAB isolates from wild Sumatran orangutans have 93% homology to W. cibaria.

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Section: Discussioncontrasting

confidence: 91%

Weissella, a novel lactic acid bacteria isolated from wild Sumatran orangutans (Pongo abelii)

et al. 2019

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“…The initial identification of the isolates was done by using gram staining, microscopic examination, and the carbohydrate fermentation test, using API 50 CH carbohydrate fermentation strips (bioMerieux, Craponne, France), and was based on phenotypical characteristics (Brolazo et al 2011). The results were analyzed according to the biochemical profiles registered in the APIweb ® database (bioMerieux).…”

Section: Identification Of Strainsmentioning

confidence: 99%

Optimizing the fermentation condition of low salted squid jeotgal by lactic acid bacteria with enhanced antioxidant activity

Akther

Chung

2017

JABC

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Lactic acid bacteria (LAB) are widely used as starter culture in food fermentation due to their harmless entity and health beneficial properties along with the ability to change texture, aroma, flavor and acidity of food products. In this study, five different LAB (FB003, FB058, FB077, FB081, and FB111) isolated from different Korean traditional fermented foods, assigned to Lactobacillus plantarum, Pediococcus pentosaceus, Weissella viridescens, Lactobacillus sakei, and Leuconostoc mesenteroides, respectively, on the basis of their physiological properties and 16S rRNA sequence analysis, to use as fermentation starter and check their ability to fasten the ripening time as well as the overall optimization in the fermentation condition. To check their suitability as starters, their safety, acid and bile tolerance, NaCl and temperature resistance, susceptibility to common antibiotics, and antimicrobial activities were determined. Squid jeotgal samples were prepared by adding 10 8 CFU/g of each strain in different samples, which were then kept for fermentation at 4 o C and checked for their antioxidant activities at 0, 7, 15, and 21-day intervals. The samples fermented with FB003 and FB077 displayed the highest antioxidant activity. This study revealed two effective starter cultures (FB003, FB077) for squid jeotgal fermentation, which presented increased functionalities. The results of this study will lead to the development of novel industrial-scale production avenues for jeotgal preparation, and offer new insights into the prevention and control of chronic diseases.

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“…Similar incongruity situation reported for Lactobacillus crispatus which determined as Lactobacillus acidophilus by API test. The test may lead to provocative results and the reliability was being argued (Brolazo et al, 2011). Bahrami and Sjöholm (2016) supported that characterization by API 50 CH is not reliable enough by the fact that the database is not updated, and some new species are not available.…”

Section: Phenotypic and Genotypic Identification Of Lactobacillus Sppmentioning

confidence: 99%

Human milk as a potential source for isolation of probiotic lactic acid bacteria: a mini review

Mustakim¹,

Sinawat²,

Salleh³

et al. 2019

Food Res.

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Probiotics are living microorganism that can be employed as a new approach to promote human health. These organisms are found to have attractive means for health due to their probiotic properties particularly in generating antimicrobial activity. Lactobacillus spp. is one of the main genera commonly used for probiotic purpose. Human milk is a potential source of Lactobacillus spp. and one of the criteria that found beneficial is that it is of human origin, which could be more reliable sources to be used in human. Lack of studies on isolation of probiotic bacteria from human milk was reported and some probiotic properties show a variation between strains from different regions and population. Therefore, it is important to carry out the isolation of Lactobacillus spp. from a large number of species in the genera to facilitate the finding of the most competent strain to be incorporated as probiotic agent. Moreover, to ensure suitability and compatibility for human use, the probiotic agent originated from human milk should not be an exception. In addition, certain probiotics show a great correlation with prebiotic existed in human milk to boost their function and may suggest an added value to be a suitable candidate as probiotic. This review provides an overview of studies related to human milk as the promising sources for isolation of probiotic microorganisms.

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Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates

Cited by 22 publications

References 20 publications

Weissella, a novel lactic acid bacteria isolated from wild Sumatran orangutans (Pongo abelii)

Weissella, a novel lactic acid bacteria isolated from wild Sumatran orangutans (Pongo abelii)

Optimizing the fermentation condition of low salted squid jeotgal by lactic acid bacteria with enhanced antioxidant activity

Human milk as a potential source for isolation of probiotic lactic acid bacteria: a mini review

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