Correlation Between Antioxidant Property and Total Phenolic and Flavonoid Content of Azadirachta indica A Juss Flowers

Abstract

The present study investigated the antioxidant activity of shade dried neem flower powder extracts by DPPH, phospomolybdenum, and FRAP methods respectively. The antioxidant activity of different extracts of neem flower powder has been investigated. Among them, 80% hydroalcoholic extract displayed the highest scavenging activity with the IC50 value of 18 mg/L in phospomolybdenum assay, 29 mg/L in DPPH, 30 mg/L in FRAP assay. Whereas Pet. ether (PE) extract shows lowest antioxidant potential amongst them with the IC50 value of 76 mg/L in FRAP, 37 mg/L DPPH, 28 mg/L in phospomolybdenum assay. On examining the total phenolic and flavonoid content of extracts, 80% hydroalcoholic extract possesses the greatest phenolic content about 83.88 mg/L followed by methanol, acetone and PE about 62.05, 7.52, 3.56 mg/L respectively. The highest flavonoid content of 57.36 mg/L in 80% hydroalcoholic extract followed by methanol, acetone and PE about 20.56, 11.25, 1.56 mg/L respectively. The order of total phenolic and flavonoid content of extracts are found to be: 80% hydroalcoholic > methanol > acetone > pet. ether respectively. The correlation between the antioxidant and total phenolic and flavonoid content was calculated using Prism Pad software and observed at 95% confidence interval level. From the correlation graph, it is observed that there is a good correlation between antioxidant, total phenolic (phospomolybdenum assay, R2 – 0.702), and total flavonoid (DPPH assay, R2 – 0.665) content, and it is possible to suggest that total phenolic and flavonoid content of the plant extracts are the reason for the antioxidant activity.

Hydroalcoholic extract: A potential source from Azadirachta indica a Juss flower for DNA and BSA binding, scavenging, fungicidal, and larvicidal activities

“…From the literature, it is noted that phytochemicals contribute to antioxidant and antimicrobial Properties. The presence of these phytochemicals was further confirmed by high‐resolution analytical methods (GC–MS & LC–MS) that had already been reported by our groups (Mohansundaram & Mary Saral, 2022).…”

“…The presence of these phytochemicals was further confirmed by high-resolution analytical methods (GC-MS & LC-MS) that had already been reported by our groups (Mohansundaram & Mary Saral, 2022).…”

“…As per the methodologies that have been reported by us (Mohansundaram & Mary Saral, 2022), the extraction procedure was carried out. The different solvent extraction through soxhlet has been done with shade‐dried neem flowers collected from a single neem tree.…”

Phytochemical Screening, Antibacterial, Antifungal, Anti‐Biofilm and Antioxidant Activity of Azadiracta Indica A. Juss. Flowers

“…The calculated R2 values reveal that the polyphenols which are present in HA extracts can play a major role in their scavenging activity and also antimicrobial properties as reported earlier by us. [37] The statistical analysis proves that there is a good correlation between the Antioxidant and total polyphenol content of HA extract [R2 = 0.79]. Hence, it is evident that the antibacterial activity of the HA extracts can be due to the presence of polyphenols.…”

“…According to established protocols reported [37] the phytochemical screening of all the extracts were carried out to examine for the presence of secondary metabolites.…”