Correlation between antibodies to type II collagen and treatment outcome in bilateral progressive sensorineural hearing loss

Helfgott, Simon M.; Lorenzo, C. Olveira; Kieval, Raphael I.; Trentham, David E.; Mosciscki, Ra; Martín, Julia San; McKenna, Michael J.; Nadol, Joseph B.

doi:10.1016/0140-6736(91)91165-q

Cited by 55 publications

(20 citation statements)

References 18 publications

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“…The different collagens might be particularly important for processes that sculpt the elaborate compartments of the inner ear during morphogenesis. This speculation is corroborated by the observation that perturbations of collagen metabolism lead to abnormal aural structure (31) and that autoimmune responses against type II and IX collagens culminate in progressive hearing loss (32,33). Candidate Genes for Hearing Disorders.…”

Section: Discussionmentioning

confidence: 62%

“…Twenty-five micrograms of denatured total RNA was separated on 1% formaldehyde-agarose gels. The RNA was transferred (11) to Hybond-N (Amersham) and incubated at 70°C with randomly primed (10), 32 P-labeled probes in hybridization solution (ExpressHyb, CLONTECH). After having been washed with 0.1% SDS in 0.2ϫ SSC at 68°C, the filters were exposed to film (XAR-5, Eastman Kodak).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular markers for cell types of the inner ear and candidate genes for hearing disorders

Heller

Sheane

Javed

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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To identify genes expressed in the vertebrate inner ear, we have established an assay that allows rapid analysis of the differential expression pattern of mRNAs derived from an auditory epithelium-specific cDNA library. We performed subtractive hybridization to create an enriched probe, which then was used to screen the cDNA library. After digoxigenin-labeled antisense cRNAs had been transcribed from hybridization-positive clones, we conducted in situ hybridization on slides bearing cryosections of late embryonic chicken heads, bodies, and cochleae. One hundred and twenty of the 196 clones analyzed encode 12 proteins whose mRNAs are specifically or highly expressed in the chicken's inner ear; the remainder encode proteins that occur more widely. We identified proteins that have been described previously as expressed in the inner ear, such as ␤-tectorin, calbindin, and type II collagen. A second group of proteins abundant in the inner ear includes five additional types of collagens. A third group, including Coch-5B2 and an ear-specific connexin, comprises proteins whose human equivalents are candidates to account for hearing disorders. This group also includes proteins expressed in two unique cell types of the inner ear, homogene cells and cells of the tegmentum vasculosum.Developmental analysis of the inner ear is hindered by the paucity of molecular markers for specific cell types. At present, most cells in the cochlea and vestibular organs cannot be unambiguously identified before they display their characteristic mature forms. The cloning of cDNAs that encode proteins specific to hair cells, supporting cells, and other cells of the inner ear is, in turn, retarded by the limited numbers of these cells in an animal. This disadvantage potentially can be circumvented through enrichment of specific cDNAs by subtractive hybridization (1-3) and library normalization (4). That only a few inner ear-specific cDNAs have been obtained from libraries constructed by this approach (2, 3), however, suggests that the efficient cloning of cDNAs specific to sensoryepithelial cell types requires libraries constructed from more abundant sources than whole cochleae. To address this problem, we constructed a cDNA library from sensory epithelia of the chicken's cochlea and developed an enriched probe with which to identify cDNAs encoding proteins expressed by specific cell types of the inner ear.The identification of proteins specific to the internal ear is also potentially useful in the study of heritable human deafness, which afflicts about one individual in a thousand. Several proteins that have been identified as markers for specific aural cell types recently have been advanced as candidate genes for human hearing disorders (for reviews see refs. 5-8). Some of the genes identified by the strategy described in this paper are likely to have human equivalents that cause hearing disorders when mutated. MATERIALS AND METHODSLibrary Construction and Screening. Basilar papillae from 250 late-embryonic (E, embryonic day) (8% E14...

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Section: Discussionmentioning

confidence: 62%

Section: Methodsmentioning

confidence: 99%

Molecular markers for cell types of the inner ear and candidate genes for hearing disorders

Heller

Sheane

Javed

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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“…Nevertheless, Lolov et al [56] reported somewhat increased serum autoantibody concentrations in the sera of patients with advanced disease. Later, Joliat et al [57], Bujía et al [58] and Helfgott et al [59] detected elevated serum levels of autoantibodies to type II and IX collagens in the sera of patients with otosclerosis compared to controls. Yoo et al [60] reported that type II collagen-specific autoimmune reaction induced in rats may cause lytic bone lesions in the otic capsule that highly resemble otosclerosis.…”

Section: Autoimmunity and Inflammationmentioning

confidence: 98%

Etiopathogenesis of otosclerosis

Karosi

Sziklai

2010

Eur Arch Otorhinolaryngol

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The objectives of our study was to review our current knowledge of the etiopathogenesis of otosclerotic bone remodeling including genetics, viral infection, autoimmunity and inflammation and to discuss disease pathogenesis with relevance to pharmacotherapy. Relevant publications on the etiopathogenesis, molecular biology, genetics and histopathology of otosclerosis from 1984 to 2009 were analyzed. Otosclerosis is a bone remodeling disorder of the human otic capsule; however, the etiopathogenesis remains unclear. Genetic predisposition, disturbed bone metabolism, persistent measles virus infection, autoimmunity, and hormonal and environmental factors also may play contributing roles in the pathogenesis of otosclerosis. Since diagnosis of otosclerosis is still based on histopathological examination of the removed stapes footplate, systemic prospective studies based on comprehensive histopathological and molecular biological analysis are necessary to obtain further information on the background of the disease.

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“…Our laboratory has reported that type-II collagen might have a key role as the inner-ear antigen in the etiology of Me niere's disease and otosclerosis [3. 8], Helfgott et al [9] suggested that type-II collagen may be important in the pathogenesis of bilateral PSNHL.…”

Section: Introductionmentioning

confidence: 99%

Antibodies against Inner-Ear Proteins in the Sera of Patients with Inner-Ear Diseases

Suzuki

Krug²,

Cheng³

et al. 1997

ORL

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Sera from patients with various inner-ear diseases, especially Ménière’s disease, were investigated by Western blot against guinea pig inner-ear proteins. Of 45 patients, 24 (53%) with various inner-ear diseases had antibodies against inner-ear proteins, compared with 0 of 10 (0%) in control subjects without inner-ear diseases. Of the 10 proteins that showed a positive reaction with patient sera, the 28-kD band was unique in that it appeared only in the membranous fraction of the inner ear and was highly positive (28%) in reaction with Ménière’s disease patient sera. The results in the present study with proteins extracted from guinea pig inner ear were consistent with our previous study using proteins from human inner ear, suggesting that the 28-kD protein may be a candidate for detecting autoimmune inner-ear disease.

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Correlation between antibodies to type II collagen and treatment outcome in bilateral progressive sensorineural hearing loss

Cited by 55 publications

References 18 publications

Molecular markers for cell types of the inner ear and candidate genes for hearing disorders

Molecular markers for cell types of the inner ear and candidate genes for hearing disorders

Etiopathogenesis of otosclerosis

Antibodies against Inner-Ear Proteins in the Sera of Patients with Inner-Ear Diseases

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