Correlation between adhesion, enzyme production, and susceptibility to fluconazole in Candida albicans obtained from denture wearers

Lyon, Juliana Pereira; Resende, Maria Aparecida de

doi:10.1016/j.tripleo.2005.12.015

Cited by 67 publications

(71 citation statements)

References 32 publications

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“…29 This finding could be attributed to the strain used by these authors, as clinical strains isolated from infections showed increased virulence factors. 32 The findings of the present investigation partially corroborate those of Teichert et al, 27 as these authors also observed yeast and pseudohyphae limited to the keratinized layer, but lack of inflammatory changes in mice not submitted to PDT and subepithelial inflammatory infiltrate and neutrophilic exocytosis after PDT. Moreover, the different mouse strain, time of assays, PS, and light source used may also explain the divergence between the histological findings in these studies.…”

Section: Discussionsupporting

confidence: 91%

“…30,31 Another investigation showed that isolates of Candida obtained from denture wearers with signs of denture stomatitis were more adherent to epithelial cells than those obtained from healthy patients. 32 In addition, Sanitá et al 33 suggested that clinical isolates of Candida spp. can have significantly higher adherence to denture base materials than ATCC strains.…”

Section: Discussionmentioning

confidence: 99%

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Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Mima

Pavarina

Dovigo

et al. 2010

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

138

129

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Objective. In vivo studies of antimicrobial PDT in animal models of oral candidosis are scarce and the association of porphyrin and LED light has not been evaluated for in vivo photoinactivation of Candida. In this study the effectiveness of photodynamic therapy (PDT) on the inactivation of Candida albicans in vivo was evaluated. Study design. Seventy-one 6-week-old female Swiss mice were immunosuppressed, provided tetracycline to their drinking water, then orally swabbed with a suspension of C. albicans (10 7 CFU/mL). Four days after oral inoculation, PDT was performed on the dorsum of the tongue after topical administration of Photogem at 400, 500, or 1000 mg/L and followed 30 minutes later by illumination with LED light (305 J/cm 2 ) at 455 or 630 nm (n ϭ 5 each). After swabbing to recover yeast from the tongue, the number of surviving yeast cells was determined (CFU/mL) and analyzed by ANOVA and Holm-Sidak tests (P Ͻ .05). Animals were humanely killed, and the tongues surgically removed and processed for histological evaluation of presence of yeast and inflammatory reaction. Results. PDT resulted in a significant reduction in C. albicans recovered from the tongue (P Ͻ .001) when compared with mice from the positive control group. There was no difference between the concentrations of Photogem and LED light wavelengths used. Histological evaluation of the tongue revealed that PDT causes no significant adverse effects to the local mucosa. Conclusion. PDT promoted significant reduction in the viability of C. albicans biofilm without harming the tongue tissue.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Mima

Pavarina

Dovigo

et al. 2010

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

138

129

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“…Some clinical research has used some chlorhexidinebased (CLX) oral rinses as an alternative to antifungal agents used regularly, obtaining good results (10); while other studies have shown an increase of pathogenicity in strains treated with fluconazole (FLZ) in a model of systemic infection in rats (11). Other authors have shown an important FLZ effect on the capacity of adhesion and production of exoenzymes (12). One of the new biomaterials being used in Dentistry is chitosan (HMWC), a cationic polymer obtained from chitin (13).…”

Section: Introductionmentioning

confidence: 99%

Antifungal and post-antifungal effects of chlorhexidine, fluconazole, chitosan and its combinations on Candida albicans

Se¹,

Ma²,

Barembaum³

et al. 2011

Med Oral

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Objective: The aim of this work was to assess the antifungal and post-antifungal effects of chlorhexidine, fluconazole, chitosan and its combinations on virulence factors of Candida albicans. Study Design: Ten isolated strains of Candida albicans obtained from 10 patients with oral candidiasis and a collection strain of C. albicans were treated with antifungal agents in different concentrations or combinations of them. Virulence factors analyzed were the cell surface hydrophobicity, the germinative tube development, the phospholipase activity and the post-antifungal effect of that exposure. Results: Virulence factors of the isolated strains obtained from patients together with the collection strain showed significant decreases with the different antifungal treatments, except for hydrophobicity and phospholipase activity. The development of germinative tube was the most sensitive factor to all the antifungal agents used. Untreated strains as well as the ones treated with antifungal agents showed a positive correlation among the virulence factors analyzed. No synergic effects arose from the combinations of the used drugs. Conclusions: C. albicans isolated strains from patients showed high phospholipase activity and germinative tube production, which corroborates their capacity to infect the oral mucosa and the high prevalence of species. As a whole, our results imply that short exposures to sub-inhibitory concentrations of the antifungal agents under analysis, isolated or combined, can modulate the way virulence factors get manifested, thus decreasing their pathogenicity.

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“…However, Takakura et al [28], who first described this murine model of oral candidosis, observed the destruction of several epithelial layers, which may be attributed to the clinical isolate strain of C. albicans used to induce oral infection. Clinical isolates can show increased virulence factors over a reference strain, such as that used in the present investigation [34,35].…”

Section: Discussionmentioning

confidence: 78%

The influence of photodynamic therapy parameters on the inactivation ofCandidaspp:in vitroandin vivostudies

Alves¹,

Mima²,

Dovigo³

et al. 2014

Laser Phys.

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The influence of parameters of photodynamic therapy (PDT), such as pre-irradiation time (PIT), on the inactivation of Candida spp. was assessed in vitro and in vivo. Suspensions of Candida albicans, Candida tropicalis, Candida krusei and Candida glabrata were treated with Photogem R , incubated for 5, 10 or 15 min and illuminated with a blue LED light. Colonies were cultivated and log values of CFU ml −1 were analyzed by ANOVA and Kruskall-Wallis test. For in vivo evaluation, immunosuppressed mice were inoculated with C. albicans. PDT was performed on the dorsum of the tongue by topical administration of Photogem R and illumination after 5, 10 or 15 min. C. albicans was recovered from the tongue and the number of CFU ml −1 was analyzed by ANOVA and Tukey test. Animals were killed and the tongues were surgically removed for histological analysis. Susceptibility of Candida spp. suspensions to PDT was in decreasing order: C. albicans = C. tropicalis < C. krusei < C. glabrata. No significant difference was observed among the different PIT ( p > 0.05), both in vivo and in vitro. A significant reduction ( p < 0.05) of log(CFU ml −1 ) of C. albicans from tongues of mice was observed with no adverse effects in the tissue. PDT was effective to inactivate in vitro Candida spp. and for reduction of C. albicans in vivo, independently of the PIT used.

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Correlation between adhesion, enzyme production, and susceptibility to fluconazole in Candida albicans obtained from denture wearers

Cited by 67 publications

References 32 publications

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Antifungal and post-antifungal effects of chlorhexidine, fluconazole, chitosan and its combinations on Candida albicans

The influence of photodynamic therapy parameters on the inactivation ofCandidaspp:in vitroandin vivostudies

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