Objective: The aim of this work was to assess the antifungal and post-antifungal effects of chlorhexidine, fluconazole, chitosan and its combinations on virulence factors of Candida albicans. Study Design: Ten isolated strains of Candida albicans obtained from 10 patients with oral candidiasis and a collection strain of C. albicans were treated with antifungal agents in different concentrations or combinations of them. Virulence factors analyzed were the cell surface hydrophobicity, the germinative tube development, the phospholipase activity and the post-antifungal effect of that exposure. Results: Virulence factors of the isolated strains obtained from patients together with the collection strain showed significant decreases with the different antifungal treatments, except for hydrophobicity and phospholipase activity. The development of germinative tube was the most sensitive factor to all the antifungal agents used. Untreated strains as well as the ones treated with antifungal agents showed a positive correlation among the virulence factors analyzed. No synergic effects arose from the combinations of the used drugs. Conclusions: C. albicans isolated strains from patients showed high phospholipase activity and germinative tube production, which corroborates their capacity to infect the oral mucosa and the high prevalence of species. As a whole, our results imply that short exposures to sub-inhibitory concentrations of the antifungal agents under analysis, isolated or combined, can modulate the way virulence factors get manifested, thus decreasing their pathogenicity.
were determined by ELlSA,they did not reveal statistically significant differences with regard to the control (p=0.05). The dextran production was determined at different concentrations of polymers by means of espectrofotometria (11.=520),using a suspension from 1 to 5.108 UFC mi of Sto mutans in sucrose broth 8,5%. The results showed significant reduction, to the formation of dextran with respect to the control (p<0.05), being for 32% to HMWQ0.25 g% PN and 66,8% for HMWQ0.50 g% P/V,while for Na-Al0.10 g% PN was 30% and Na-Al0.50 g% P Vwas the 32%. It is concluded that QAPMand Al-Na, with regard to their characteristic physical and chemistry that permit to form hydrogen bond Iike, and polymeric chains that confer sufficient elasticity to agree to the different structures of the oral cavity, offer expectations for the therapeutic investigation of new formulations of deontological application.
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