Correlation Analysis of New Soybean [Glycine max (L.) Merr] Gene Gm15G117700 with Oleic Acid

Qu, Shuo; Jiao, Yaolei; Abraham, Lamboro; Wang, Piwu

doi:10.32604/phyton.2021.015206

Cited by 6 publications

(5 citation statements)

References 11 publications

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“…Rong [23] constructed an expression vector containing the HSP17.4 heat shock protein gene, using the Bar gene as a selection marker, and transferred it into soybean Jinong 18 by pollen tube channel method to obtain high-temperature resistant plants, and the transformation rate was 5.83%. In order to improve the transformation efficiency, Wang et al [24] invented an improved pollen tube channel transformation method, which solved the problem of the low transformation rate of the pollen tube channel method, improved the transformation efficiency, and provided a good foundation for future soybean genetic transformation. In this experiment, the pollen tube channel method was used for genetic transformation, and the pollen tube channel transformation technology of Wang et al [24] was adopted, and the transformation rate was significantly improved.…”

Section: Discussionmentioning

confidence: 99%

“…In order to improve the transformation efficiency, Wang et al [24] invented an improved pollen tube channel transformation method, which solved the problem of the low transformation rate of the pollen tube channel method, improved the transformation efficiency, and provided a good foundation for future soybean genetic transformation. In this experiment, the pollen tube channel method was used for genetic transformation, and the pollen tube channel transformation technology of Wang et al [24] was adopted, and the transformation rate was significantly improved.…”

Section: Discussionmentioning

confidence: 99%

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Bioinformatics and Functional Analysis of High Oleic Acid-Related Gene GmSAM22 in Soybean [Glycine max (L.) Merr.]

Qu¹,

Cai²,

Cui³

et al. 2023

Phyton

Self Cite

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High yield, high quality, stable yield, adaptability to growth period, and modern mechanization are the basic requirements for crops in the 21st century. Soybean oleic acid is a natural unsaturated fatty acid with strong antioxidant properties and stability. Known as a safe fatty acid, it has the ability to successfully prevent cardiovascular and cerebrovascular disorders. Improving the fatty acid composition of soybean seeds, can not only speed up the breeding process of high-quality high-oil and high-oleic soybeans, but also have important significance in human health, and provide the possibility for the development of soybean oil as a new energy source. Hence, the aim of this study was to analyze the high oleic acid elated gene GmSAM22 in soybean. In this research the soybean oleic acid-related gene GmSAM22 was screened out by Genome-wide association analysis, a 662 bp fragment was acquired by specific PCR amplification, and the pMD18T cloning vector was linked by the use of a seamless cloning technique. Bioinformatics analysis of the signal peptide prediction, subcellular localization, protein hydrophobicity, transmembrane region analysis, a phosphorylation site, protein secondary and tertiary structure and protein interaction analysis of the protein encoded by the SAM22 gene was carried out. The plasmid of the gene editing vector is pBK041. The overexpression vector was transformed from pCAMBIA3301 as the base vector, and overexpression vector were designed. Positive plants were obtained by genetic transformation by the pollen tube channel method. Fluorescence quantitative PCR was performed on the T 2 generation plants to detect the relative expression levels in different tissues. Southern Blot was used to detect the presence of hybridization signal. Screening genes BAR, 35S, and NOS in plants were identified by conventional PCR. 10 seeds with high and low oleic acid content were chosen for quantitative PCR identification, and finally, the concentration and morphology of soybean fatty acids were identified by nearfar infrared spectroscopy. On 10 seeds with an upper and lower oleic acid content, a quantitative fluorescence analysis was done. In Southern blot hybridization, the SAM22 gene was integrated into the recipient soybean plant in hands of a sole copy. Fluorescence quantitative PCR appeared that the average relative expression of the SAM22 gene in roots, stems, leaves, and seeds was 1. 70, 1.67, 3.83, and 4.41, respectively. Positive expression seeds had a 4.77% increase in oleic acid content. The level of oleic acid in the altered seeds was reduced by 4.13% when compared to CK, and it was discovered that the GmSAM22 gene could be a regulatory and secondary gene that promotes the conversion of stearic acid to oleic acid in soybean. There has not been a discussion of gene cloning or functional verification. The cloning and genetic transformation of the soybean SAM22 gene can effectively increase the content of oleic acid, which lays a foundation for the study of soybean with high oleic acid.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Bioinformatics and Functional Analysis of High Oleic Acid-Related Gene GmSAM22 in Soybean [Glycine max (L.) Merr.]

Qu¹,

Cai²,

Cui³

et al. 2023

Phyton

Self Cite

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“…In 2021, Qu et al. analyzed the oleic acid contents of soybean plants over-expressing Gm15G117700 and soybean plants in which the gene was edited; the oleic acid content increased in the gene-edited plants by 3.49% ( Qu et al., 2021 ). Zhou et al.…”

Section: Application Of Crispr/cas9 Gene Editing Technology In Soybea...mentioning

confidence: 99%

“…Zhang et al (2019) silenced the soybean phospholipase D1encoding gene, which increased the oil content and germination rate of the mutant seeds (compared with the wild-type seeds) at high temperatures and high humidity levels (Zhang et al, 2019). In 2021, Qu et al analyzed the oleic acid contents of soybean plants over-expressing Gm15G117700 and soybean plants in which the gene was edited; the oleic acid content increased in the gene-edited plants by 3.49% (Qu et al, 2021). Zhou et al (2023a) recently edited five important enzyme-encoding genes in the GmFAD2 family and analyzed the associated effects on soybean oil synthesis.…”

Section: Improvement Of Seed Quality In Soybeanmentioning

confidence: 99%

Advances in CRISPR/Cas9-based research related to soybean [Glycine max (Linn.) Merr] molecular breeding

Yao,

Zhou,

Zhang

et al. 2023

Front. Plant Sci.

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Soybean [Glycine max (Linn.) Merr] is a source of plant-based proteins and an essential oilseed crop and industrial raw material. The increase in the demand for soybeans due to societal changes has coincided with the increase in the breeding of soybean varieties with enhanced traits. Earlier gene editing technologies involved zinc finger nucleases and transcription activator-like effector nucleases, but the third-generation gene editing technology uses clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9). The rapid development of CRISPR/Cas9 technology has made it one of the most effective, straightforward, affordable, and user-friendly technologies for targeted gene editing. This review summarizes the application of CRISPR/Cas9 technology in soybean molecular breeding. More specifically, it provides an overview of the genes that have been targeted, the type of editing that occurs, the mechanism of action, and the efficiency of gene editing. Furthermore, suggestions for enhancing and accelerating the molecular breeding of novel soybean varieties with ideal traits (e.g., high yield, high quality, and durable disease resistance) are included.

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“…Extracting the leaf genomic DNA of the T 2 generation soybean transformed plants and untransformed plants identified as positive using PCR with pCAMBIA3301-sHSP26 plasmid, and the restriction enzyme Hind Ш for single enzyme digestion, and Southern blotting was used to detect the gene integration. Specific steps see Lin et al [15].…”

Section: Southern Blot Hybridization Identification Of T 2 Transgenic...mentioning

confidence: 99%

Cloning of the Soybean sHSP26 Gene and Analysis of Its Drought Resistance

Liu¹,

Liu²,

Zhang³

et al. 2022

Phyton

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Exploring the molecular mechanism of soybean response to drought stress, providing a basis for genetic improvement and breeding of heat-resistant varieties, relying on the transcriptome sequencing data of unpollinated ovary at the seven-leaf stage of soybean Jinong 18(JN18) and Jinong 18 mutant (JB18) soybeans, using reverse transcription, one gene in the sHSP family was cloned using PCR (RT-PCR) and it was named sHSP26. In this experiment, the soybean sHSP26 gene was successfully cloned by RT-PCR, the protein encoded by the sHSP26 gene was analyzed by bioinformatics, and the sHSP26 gene overexpression vector and CRISPR/Cas9 gene-editing vector were constructed. The positive plants were derived from Agrobacterium-mediated transformation of soybean cotyledon nodes, and T 2 plants were identified through conventional PCR, QT-PCR, and Southern blot hybridization. Finally, through the determination of drought-related physiological and biochemical indicators and the analysis of agronomic traits, further research on gene function was conducted. The results indicated that the overexpression vector plant GmsHSP26 gene expression increased. After stress, the SOD and POD activities, and the PRO content of the transgenic overexpression plants increased, while the MDA content decreased. The reverse was true for soybean plants with genetically modified editing vectors. A survey of agronomic traits indicated that the fourpod ratio and yield per plant of the transgenic overexpression plants were higher than those of the control and transgenic editing vector soybean plants. It indicates that the expression of the sHSP26 gene can enhance drought resistance and soybean yield. The soybean sHSP26 gene cloning and its functional verification have not yet been reported. This is the first report where PCR amplification of soybean sHSP26 genes and gene expression vector were applied. It lays the foundation for creating new drought-resistant transgenic soybean lines through genetic engineering technology and is essential for improving soybean yield and quality.

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Correlation Analysis of New Soybean [Glycine max (L.) Merr] Gene Gm15G117700 with Oleic Acid

Cited by 6 publications

References 11 publications

Bioinformatics and Functional Analysis of High Oleic Acid-Related Gene GmSAM22 in Soybean [Glycine max (L.) Merr.]

Bioinformatics and Functional Analysis of High Oleic Acid-Related Gene GmSAM22 in Soybean [Glycine max (L.) Merr.]

Advances in CRISPR/Cas9-based research related to soybean [Glycine max (Linn.) Merr] molecular breeding

Cloning of the Soybean sHSP26 Gene and Analysis of Its Drought Resistance

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