Corrections for Background in Spectrophotometry Using Difference-in-Absorbance Values.  Application to Vitamin A.

Wilkie, J. B.

doi:10.1021/ac60210a057

Cited by 6 publications

(1 citation statement)

References 22 publications

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“…In this correction method, it is assumed that the microplate has consistent background absorption at the two different used wavelengths. Nevertheless, this is not true with the most microplates in the UV range of the spectrum and choosing the plates is important for performing the measurements (15)(16)(17). Therefore, the aim of this study was to evaluate the background absorbance characteristics of polystyrene microplates which are commonly used in most of the microplate-based assays without taking enough care regarding choosing the appropriate microplate.…”

Section: Introductionmentioning

confidence: 99%

Comparison of polystyrene versus cycloolefin microplates in absorbance measurements in the UV/VIS region of the spectrum

Abedi

Cohan

Ardestani

et al. 2019

J Shahrekord Univ Med Sci

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Background and aims: Polystyrene microplates are generally used to specify the absorption in the visible light region of the spectrum. However, they are capable of absorption in the ultraviolet (UV) range of the spectrum while they are not suitable for UV spectroscopy analysis. This study aimed to compare polystyrene and cycloolefin microplates for their background absorbance characteristics in the UV/ VIS region of the spectrum. Methods: Background absorbance of the mentioned microplates was measured using two different spectrophotometers and four various samples. Results: The analysis of our results verified the advantage of applying cycloolefin microplate over polystyrene one for absorbance measurements in the UV range of the spectrum. Conclusion: In general, suitable microplate selection is a critical factor in absorbance measurements, especially in the UV portion of the spectrum.

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Section: Introductionmentioning

confidence: 99%