Correction to: Shoot regeneration process and optimization of Agrobacterium-mediated transformation in Sinningia speciosa

Kuo, Wen-Hsi; Hung, Ying; Wu, Ho-Wei; Pan, Zhaojun; Hong, Chwan-Yang; Wang, Chun‐Neng

doi:10.1007/s11240-018-1453-2

Cited by 3 publications

(6 citation statements)

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“…Auxin alone or in combination with cytokinin was exhibited to stimulate shoot generation of plants such as Sinningia speciosa [16] and Saintpaulia [27][28][29]. In our experiments, the addition of NAA significantly improved the number and proliferation coefficient of adventitious shoots.…”

Section: Effect Of Pgrssupporting

confidence: 48%

“…Moreover, in vitro leaf explants are more suitable for genetic transformation research as they are sterile, and gene stacking technology is feasible [16,36]. Many studies have successfully established the tissue culture system using leaves as explants, such as Sinningia speciosa [16] and Saintpaulia species [27][28][29]. In our study, both field-grown leaves and aseptic seeding leaves as explants successfully induced shoot regeneration from S. hybrida by organogenesis pathway.…”

Section: Leaf As Explant Source For Regenerationmentioning

confidence: 65%

“…BA is the most effective of the four cytokinins applied in S. hybrida. Kuo and Xu reported similar outcomes in species Sinningia speciosa [13,16]. TDZ is considered as optimal synthetic cytokinin for the regeneration of many plant systems [17][18][19].…”

Section: Effect Of Pgrsmentioning

confidence: 73%

“…As a convenient and abundant raw material, leaves were used in the investigation since they usually exhibit better regeneration potential than explants from mature tissues [35]. Moreover, in vitro leaf explants are more suitable for genetic transformation research as they are sterile, and gene stacking technology is feasible [16,36]. Many studies have successfully established the tissue culture system using leaves as explants, such as Sinningia speciosa [16] and Saintpaulia species [27][28][29].…”

Section: Leaf As Explant Source For Regenerationmentioning

confidence: 99%

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Adventitious Shoot Regeneration from Leaf Explants in Sinningia Hybrida ‘Isa’s Murmur’

Yang

Wang

et al. 2022

Plants

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As a valuable ornamental plant, Sinningia hybrida ‘Isa’s Murmur’ (S. hybrida) has genetic flower diversity, which has great potential to develop different flower characters in the horticultural market. The present study focuses on establishing a practical approach for the sustainable propagation of S. hybrida. Compared with aseptic seeding leaves explants, field-grown leaves explants are more suitable for adventitious shoot regeneration. Adding 0.1 mg L−1 NAA and 2.0 mg L−1 TDZ could obtain the highest adventitious shoot proliferation coefficient (24.5), and the induction rate was 91.7%. The shoot proliferation coefficient (20.7) and the greatest shoot length and induction rate (95.3%) were achieved in 0.1 mg L−1 NAA and 2.0 mg L−1 BA medium, accompanied by rooting formation. Adding 0.5 mg L−1 GA3, 1.0 mg L−1 BA, and 0.2 mg L−1 IBA to MS medium can effectively prolong the regenerated buds for rooting. The best for rooting was 1/2 MS medium containing 0.3 mg L−1 IBA, with the maximum number of roots (13.4 per shoot) and survival rate for transplanting (100%). This work aims to build an efficient, definitive, and scalable protocol for S. hybrida regeneration useful for large-scale cultivation and even more protoplast fusion and genetic transformation to develop more colorful or fragrant flowers.

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Section: Effect Of Pgrssupporting

confidence: 48%

Section: Leaf As Explant Source For Regenerationmentioning

confidence: 65%

Section: Effect Of Pgrsmentioning

confidence: 73%

Section: Leaf As Explant Source For Regenerationmentioning

confidence: 99%

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Adventitious Shoot Regeneration from Leaf Explants in Sinningia Hybrida ‘Isa’s Murmur’

Yang

Wang

et al. 2022

Plants

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“…Leaf is often the first choice for tissue culture because of its convenience and abundance of raw materials. More important is that leaf, i.e., the basic material of genetic transformation [38,39]. Three kinds of explants, namely nodal segment, leaf, and shoot tip, were used in our study.…”

Section: Optimal Explantsmentioning

confidence: 99%

A Novel and Efficient In Vitro Organogenesis Approach for Ajuga lupulina Maxim

Yang

et al. 2021

Plants

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This work was aimed at establishing an effective approach for in vitro propagation of Ajuga lupulina Maxim, a medicinal and ornamental plant mainly found in eastern Xizang, in the western Sichuan region of China. We report an optimum response in the proliferation of axillary shoots from nodal segment explants (10.2 shoots/explant) on MS medium containing 3.0 mg L−1 of 6-benzyladenine (BA). When BA and TDZ individually or in combination with NAA were employed for adventitious shoot regeneration, shoots and embryo-like structures (ELSs) were noted in the callus from leaf explants. The maximum response of 26.4 shoots /explant (81.6%) and 12.0 ELSs/explant were ascertained on MS medium with 4.0 mg L−1 TDZ and 0.1 mg L−1 NAA. The leaf despite browning still demonstrated a high regeneration capacity. TDZ (2.0 mg L−1) and BA (2.0 mg L−1) along with NAA (0.01 mg L−1) were found to perform well for shoot regeneration via callus from shoot tip explants. The best for rooting was MS medium (half-strength) containing indole-3-butyric acid (IBA: 1.5 mg L−1) and (NAA: 0.5 mg L−1) with the maximum number of roots (25.8 per shoot) and the highest rooting frequency (81.71%). The survival of the plantlets in the greenhouse was 78.2% indicative of successful acclimatization. This work is the first report of a consistent, definitive, and unique protocol for A. lupulina regeneration, paving the way for the in vitro preservation of such significant genetic resources and also further allied systems based on such callus-based or embryo-based approaches.

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Tissue culture and Agrobacterium-mediated genetic transformation of the oil crop sunflower

Chen,

Zeng,

Cheng

et al. 2024

PLoS ONE

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Sunflower is one of the four major oil crops in the world. ‘Zaoaidatou’ (ZADT), the main variety of oil sunflower in the northwest of China, has a short growth cycle, high yield, and high resistance to abiotic stress. However, the ability to tolerate adervesity is limited. Therefore, in this study, we used the retention line of backbone parent ZADT as material to establish its tissue culture and genetic transformation system for new variety cultivating to enhance resistance and yields by molecular breeding. The combination of 0.05 mg/L IAA and 2 mg/L KT in MS was more suitable for direct induction of adventitious buds with cotyledon nodes and the addition of 0.9 mg/L IBA to MS was for adventitious rooting. On this basis, an efficient Agrobacterium tumefaciens-mediated genetic transformation system for ZADT was developed by the screening of kanamycin and optimization of transformation conditions. The rate of positive seedlings reached 8.0%, as determined by polymerase chain reaction (PCR), under the condition of 45 mg/L kanamycin, bacterial density of OD600 0.8, infection time of 30 min, and co-cultivation of three days. These efficient regeneration and genetic transformation platforms are very useful for accelerating the molecular breeding process on sunflower.

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Correction to: Shoot regeneration process and optimization of Agrobacterium-mediated transformation in Sinningia speciosa

Cited by 3 publications

References 0 publications

Adventitious Shoot Regeneration from Leaf Explants in Sinningia Hybrida ‘Isa’s Murmur’

Adventitious Shoot Regeneration from Leaf Explants in Sinningia Hybrida ‘Isa’s Murmur’

A Novel and Efficient In Vitro Organogenesis Approach for Ajuga lupulina Maxim

Tissue culture and Agrobacterium-mediated genetic transformation of the oil crop sunflower

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