Correction to: An efficient and reproducible Agrobacterium- mediated transformation method for hexaploid wheat (Triticum aestivum L.)

Hayta, Şadiye; Smedley, Mark; Demir, Selcen U.; Blundell, Robert; Hinchliffe, Alison; Atkinson, Nicola J.; Harwood, Wendy

doi:10.1186/s13007-019-0540-7

Cited by 9 publications

(3 citation statements)

References 1 publication

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“…Fielder with A. tumefaciens (strain EHA105)-mediated transformation according to the method described by Hayta et al Ten positive transgenic plants were selected based on hygromycin B resistance, from which three independent transgenic lines were selected for further experiments. A line segregated from T0 transgenic lines that did not contain the transgene was also used as a segregated nontransgenic control (NG).…”

Section: Methodsmentioning

confidence: 99%

Overexpression of Rice OsHMA3 in Wheat Greatly Decreases Cadmium Accumulation in Wheat Grains

Zhang

Cheng

Chen

et al. 2020

Environ. Sci. Technol.

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Cereals are a major dietary source of the toxic metal cadmium (Cd). Reducing Cd accumulation in cereal crops such as wheat (Triticum aestivum) is important for food safety and human health. In this study, we show that three diverse cultivars of wheat had a high Cd translocation from roots to shoots, similar to a rice (Oryza sativa) cultivar possessing a nonfunctional tonoplast Cd transporter OsHMA3. We investigated the function of TaHMA3 genes in wheat. Three TaHMA3 genes were identified in wheat, all of which encode tonoplast-localized proteins. However, heterologous expression of TaHMA3 genes in yeast showed no transport activities for Cd, which likely explains the low Cd sequestration in wheat roots and subsequently the high Cd translocation to wheat shoots. To increase Cd sequestration in wheat roots, we overexpressed a rice functional OsHMA3 gene in wheat driven by a strong constitutive Ubiquitin promoter. Overexpression of the OsHMA3 gene decreased root-to-shoot Cd translocation in wheat by nearly 10-fold and Cd accumulation in wheat grain by 96%. The results suggest that high Cd translocation is a common trait in wheat caused by a loss of the Cd transport function of TaHMA3 proteins. Transgenic wheat overexpressing a functional OsHMA3 gene offers a highly effective solution to decrease Cd accumulation in wheat grain.

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Section: Methodsmentioning

confidence: 99%

Overexpression of Rice OsHMA3 in Wheat Greatly Decreases Cadmium Accumulation in Wheat Grains

Zhang

Cheng

Chen

et al. 2020

Environ. Sci. Technol.

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“…Ebrahimzadegan and Maroufi established an Agrobacterium-mediated genetic transformation system for obtaining transgenic plants by using Lallemantia iberica cotyledonary nodes as explants [28]. Hayta et al developed and optimized an Agrobacteriummediated genetic transformation system with efficiencies of up to 25% [29]. German chamomile is an aromatic medicinal plant that belongs to the genus Matricaria in the family Asteraceae.…”

Section: Discussionmentioning

confidence: 99%

Establishment and validation of a callus tissue transformation system for German chamomile (Matricaria chamomilla L.)

Tai,

Zhang,

Chen

et al. 2023

BMC Plant Biol

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Background German chamomile (Matricaria chamomilla L.) is an important medicinal plant, and the essential oils in the flowers have various biological activities. Genetic transformation systems are important for plant quality improvement and molecular research. To the best of our knowledge, a genetic transformation system has not yet been reported for German chamomile. Results In this study, we developed Agrobacterium-mediated transformation protocols for German chamomile callus tissues. This involved optimizing key parameters, such as hygromycin and cefotaxime concentrations, bacterial density, and infection and co-culture durations. We also performed gas chromatography–mass spectrometry analysis to identify volatile compounds in non-transgenic and transgenic callus and hairy root tissues. Furthermore, to compare and verify the callus transformation system of German chamomile, we transferred McFPS to the hairy roots of German chamomile. The results showed that the optimal conditions for Agrobacterium-mediated callus tissue transformation were as follows: explant, petiole; cefotaxime concentration, 300 mg/L; hygromycin concentration, 10 mg/L; and bacterial solution concentration, OD600 = 0.6; callus transformation efficiency was the highest when the co-culture time was 3 days. Conclusions Establishment of a high-efficiency callus transformation system will lay the foundation for gene function identification in German chamomile.

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“…Transformation of wheat lines (Triticum aestivum var. Fielder) with pBract202-TaVIT2 and their initial characterization have been described in (Connorton et al, 2017;Hayta et al, 2019).…”

Section: Plant Materials and Growthmentioning

confidence: 99%

Subcellular dynamics studies reveal how tissue-specific distribution patterns of iron are established in developing wheat grains

Sheraz

Wan

Venter

et al. 2021

Preprint

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Understanding iron trafficking in plants is key to enhancing the nutritional quality of crops. Due to the difficulty of imaging iron in transit, little is known about iron translocation and distribution in developing seeds. A novel approach, combining 57Fe isotope labelling and NanoSIMS, was used to visualize iron translocation dynamics at the subcellular level in wheat grain, Triticum aestivum L. We were able to track the main route of iron from maternal tissues to the embryo through different cell types. Further evidence for this route was provided by genetically diverting iron into storage vacuoles, as confirmed by histological staining and TEM-EDS. Virtually all iron was found in intracellular bodies, indicating symplastic rather than apoplastic transport. Aleurone cells contained a new type of iron body, highly enriched in 57Fe, and most likely represents iron-nicotianamine being delivered to phytate globoids. Correlation with tissue-specific gene expression provides an updated model of iron homeostasis in cereal grains with relevance for future biofortification efforts.

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Correction to: An efficient and reproducible Agrobacterium- mediated transformation method for hexaploid wheat (Triticum aestivum L.)

Abstract: In the original publication of the article [1], a few errors were identified.

Cited by 9 publications

References 1 publication

Overexpression of Rice OsHMA3 in Wheat Greatly Decreases Cadmium Accumulation in Wheat Grains

Overexpression of Rice OsHMA3 in Wheat Greatly Decreases Cadmium Accumulation in Wheat Grains

Establishment and validation of a callus tissue transformation system for German chamomile (Matricaria chamomilla L.)

Subcellular dynamics studies reveal how tissue-specific distribution patterns of iron are established in developing wheat grains

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