Overexpression of Rice <i>OsHMA3</i> in Wheat Greatly Decreases Cadmium Accumulation in Wheat Grains

Zhang, Lingxiao; Cheng, Gao; Chen, Chuan; Zhang, Wenwen; Huang, Xin‐Yuan; Zhao, Fang‐Jie

doi:10.1021/acs.est.0c02877

Cited by 92 publications

(39 citation statements)

References 38 publications

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“…Cd accumulation in roots was enhanced by up to 110–125%. Reduction in Cd accumulation in wheat grains was observed to be up to 40-folds, while translocation of Cd from roots to shoots was decreased by approximately 10-folds ( Zhang L. et al, 2020 ). This technique offers an effective solution to reduce Cd accumulation in plants, leading to reduced health risks.…”

Section: Remediation Of Cadmium-polluted Soilsmentioning

confidence: 99%

Cadmium Phytotoxicity, Tolerance, and Advanced Remediation Approaches in Agricultural Soils; A Comprehensive Review

et al. 2022

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Cadmium (Cd) is a major environmental contaminant due to its widespread industrial use. Cd contamination of soil and water is rather classical but has emerged as a recent problem. Cd toxicity causes a range of damages to plants ranging from germination to yield suppression. Plant physiological functions, i.e., water interactions, essential mineral uptake, and photosynthesis, are also harmed by Cd. Plants have also shown metabolic changes because of Cd exposure either as direct impact on enzymes or other metabolites, or because of its propensity to produce reactive oxygen species, which can induce oxidative stress. In recent years, there has been increased interest in the potential of plants with ability to accumulate or stabilize Cd compounds for bioremediation of Cd pollution. Here, we critically review the chemistry of Cd and its dynamics in soil and the rhizosphere, toxic effects on plant growth, and yield formation. To conserve the environment and resources, chemical/biological remediation processes for Cd and their efficacy have been summarized in this review. Modulation of plant growth regulators such as cytokinins, ethylene, gibberellins, auxins, abscisic acid, polyamines, jasmonic acid, brassinosteroids, and nitric oxide has been highlighted. Development of plant genotypes with restricted Cd uptake and reduced accumulation in edible portions by conventional and marker-assisted breeding are also presented. In this regard, use of molecular techniques including identification of QTLs, CRISPR/Cas9, and functional genomics to enhance the adverse impacts of Cd in plants may be quite helpful. The review’s results should aid in the development of novel and suitable solutions for limiting Cd bioavailability and toxicity, as well as the long-term management of Cd-polluted soils, therefore reducing environmental and human health hazards.

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Section: Remediation Of Cadmium-polluted Soilsmentioning

confidence: 99%

Cadmium Phytotoxicity, Tolerance, and Advanced Remediation Approaches in Agricultural Soils; A Comprehensive Review

et al. 2022

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“…HMA3 is a tonoplast-localized transporter for Cd 2+ in the roots and limited Cd 2+ in roots (Sasaki et al 2014). Overexpression of rice OsHMA3 in wheat greatly decreases Cd accumulation in wheat grain (Zhang et al 2020). Some members of ATP-binding cassette (ABC) and calcium/H + exchanger (CAX) transporters are also involved in the vacuolar Cd sequestration (Park et al 2012;Zhang et al 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Cd is highly transferable in soils, thereby enabling it to be easily taken up by roots and rapidly transported to various plant tissues. Excessive Cd accumulation in plants can not only hamper plant growth and productivity but also eventually threaten animal and human health through the food chain (Zhang et al 2020). Understanding the mechanisms controlling Cd distribution in planta is essential to develop phytoremediation approaches as well as for food safety.…”

Section: Introductionmentioning

confidence: 99%

Multiomics reveals an essential role of long-distance cadmium translocation in regulating plant cadmium resistance and grain cadmium accumulation in allohexaploid wheat (Triticum aestivum L.) genotypes

Hua

Chen

Shen

et al. 2022

Preprint

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Cadmium (Cd) easily enters cereal roots and is translocated to the shoots and grains thus posing risks to human health. Vastness and complexity of the allothexaploid wheat genome is a huge challenge for understanding its Cd resistance and accumulation. Herein, wheat plants of a Cd-resistant genotype (‘ZM1860’) and a Cd-sensitive genotype (‘ZM32’), selected from 442 wheat cultivars, exhibited significantly differential plant Cd resistance. Integrated comparative analyses of morpho-physiological investigation, ionomic and phytohormone profiling, genomic variations, transcriptomic landscapes, and functional analysis was performed to identify the mechanisms underlying differential Cd resistance. Under Cd toxicity, ‘ZM1860’ outperformed than ‘ZM32’ with obvious leaf chlorosis, weaker root architecture, ROS over-accumulation, and disordered phytohormone homeostasis. Ionomics showed that ‘ZM32’ had a higher translocation coefficient of Cd from roots to shoots and accumulated more Cd in grains than ‘ZM1860’. Whole-genome re-sequencing (WGS) and transcriptome sequencing identified numerous DNA variants and differentially expressed genes involved in abiotic stress responses and ion transport. Integrated ionomics, transcriptomics, and functional analyses identified the plasma membrane-localized TaHMA2b-7A as a central Cd exporter regulating differential long-distance Cd translocation in wheat genotypes. WGS- and PCR-based sequence polymorphisms revealed a 25-bp InDel site in the promoter region that might determine the differential gene expression of TaHMA2b-7A with same amino acid sequences in wheat gonotypes. The multi-omics approach used here eased the identification of wheat core transporters involving long-distance Cd translocation, and may provide elite gene resources for the genetic improvement of plant Cd resistance and grain Cd accumulation in wheat and other cereal species.

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“…Using genome-wide association scans, Guttieri et al (2015) identified Cd-associated single-nucleotide polymorphisms (SNPs) on 5AL in a region homologous to Cdu1 locus on 5BL in durum wheat. Zhang et al (2020) found three TaHMA3 genes (i.e., TaHMA3-A1 , TaHMA3-B1 , and TaHMA3-D1 ) in common wheat, all of which encode transporters located in the vacuolar membrane. The absolute expression level of these genes was very low in all three wheat cultivars compared with that of OsHMA3 in rice, especially in the roots.…”

Section: Introductionmentioning

confidence: 99%

Novel Quantitative Trait Loci for Grain Cadmium Content Identified in Hard White Spring Wheat

et al. 2021

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Cadmium (Cd) is a heavy metal that can cause a variety of adverse effects on human health, including cancer. Wheat comprises approximately 20% of the human diet worldwide; therefore, reducing the concentrations of Cd in wheat grain will have significant impacts on the intake of Cd in food products. The tests for measuring the Cd content in grain are costly, and the content is affected significantly by soil pH. To facilitate breeding for low Cd content, this study sought to identify quantitative trait loci (QTL) and associated molecular markers that can be used in molecular breeding. One spring wheat population of 181 doubled haploid lines (DHLs), which was derived from a cross between two hard white spring wheat cultivars “UI Platinum” (UIP) and “LCS Star” (LCS), was assessed for the Cd content in grain in multiple field trials in Southeast Idaho, United States. Three major QTL regions, namely, QCd.uia2-5B, QCd.uia2-7B, and QCd.uia2-7D, were identified on chromosomes 5B, 7B, and 7D, respectively. All genes in these three QTL regions were identified from the NCBI database. However, three genes related to the uptake and transport of Cd were used in the candidate gene analysis. The sequences of TraesCS5B02G388000 (TaHMA3) in the QCd.uia2-5B region and TraesCS7B02G320900 (TaHMA2) and TraesCS7B02G322900 (TaMSRMK3) in the QCd.uia2-7B region were compared between UIP and LCS. TaHMA2 on 7B is proposed for the first time as a candidate gene for grain Cd content in wheat. A KASP marker associated with this gene was developed and it will be further validated in near-isogenic lines via a gene-editing system in future studies.

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Overexpression of Rice OsHMA3 in Wheat Greatly Decreases Cadmium Accumulation in Wheat Grains

Cited by 92 publications

References 38 publications

Cadmium Phytotoxicity, Tolerance, and Advanced Remediation Approaches in Agricultural Soils; A Comprehensive Review

Cadmium Phytotoxicity, Tolerance, and Advanced Remediation Approaches in Agricultural Soils; A Comprehensive Review

Multiomics reveals an essential role of long-distance cadmium translocation in regulating plant cadmium resistance and grain cadmium accumulation in allohexaploid wheat (Triticum aestivum L.) genotypes

Novel Quantitative Trait Loci for Grain Cadmium Content Identified in Hard White Spring Wheat

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