Correction: The ArcAB two-component regulatory system promotes resistance to reactive oxygen species and systemic infection by Salmonella Typhimurium

Pardo-Esté, Coral; Hidalgo, Alejandro A.; Aguirre, Camila; Inostroza, Ana; Briones, Alan C.; Cabezas, Carolina; Castro‐Severyn, Juan; Fuentes, Juan A.; Opazo, María Cecilia; Riedel, Claudia A.; Otero, Carolina; Pacheco, Rodrigo; Valvano, Miguel A.; Saavedra, Claudia P.

doi:10.1371/journal.pone.0214634

“…Many bacterial TCSs, have been involved in intracellular survival mechanisms in eukaryotic cells, such as EvgSA in Shigella flexneri [54]; ArcAB [55], PhoPQ [56] and EnvZ/OmpR [57] in Salmonella Thyphimurium; SrrAB [58]; GraSR [59] in Staphylococcus aureus; PhoPQ in Escherichia coli [60]; BvrSR in Brucella abortus [61]; and PrrAB in Mycobacterium tuberculosis [62], among others. In S. pneumoniae, most of the TCSs are required for full virulence in animal models of infection [63,64] and we have shown that two of these systems, StkP/ComE and CiaRH [15,16], are important in response to acidic and/or oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

“…Many bacterial TCSs, have been involved in intracellular survival mechanisms in eukaryotic cells, such as EvgSA in Shigella flexneri [ 54 ]; ArcAB [ 55 ], PhoPQ [ 56 ] and EnvZ/OmpR [ 57 ] in Salmonella Thyphimurium; SrrAB [ 58 ]; GraSR [ 59 ] in Staphylococcus aureus ; PhoPQ in Escherichia coli [ 60 ]; BvrSR in Brucella abortus [ 61 ]; and PrrAB in Mycobacterium tuberculosis [ 62 ], among others. In S .…”

Section: Discussionmentioning

confidence: 99%

The pneumococcal two-component system SirRH is linked to enhanced intracellular survival of Streptococcus pneumoniae in influenza-infected pulmonary cells

Reinoso-Vizcaíno

¹

,

Cian

²

,

Cortes

³

et al. 2020

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The virus-bacterial synergism implicated in secondary bacterial infections caused by Streptococcus pneumoniae following infection with epidemic or pandemic influenza A virus (IAV) is well documented. However, the molecular mechanisms behind such synergism remain largely ill-defined. In pneumocytes infected with influenza A virus, subsequent infection with S. pneumoniae leads to enhanced pneumococcal intracellular survival. The pneumococcal two-component system SirRH appears essential for such enhanced survival. Through comparative transcriptomic analysis between the ΔsirR and wt strains, a list of 179 differentially expressed genes was defined. Among those, the clpL protein chaperone gene and the psaB Mn +2 transporter gene, which are involved in the stress response, are important in enhancing S. pneumoniae survival in influenza-infected cells. The ΔsirR, ΔclpL and ΔpsaB deletion mutants display increased susceptibility to acidic and oxidative stress and no enhancement of intracellular survival in IAV-infected pneumocyte cells. These results suggest that the SirRH two-component system senses IAV-induced stress conditions and controls adaptive responses that allow survival of S. pneumoniae in IAV-infected pneumocytes.

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“…Many bacterial TCSs, have been involved in intracellular survival mechanisms in eukaryotic cells, such as EvgSA in Shigella flexneri [49]; ArcAB [50], PhoPQ [51] and EnvZ/OmpR [52] in Salmonella thyphimurium ; SrrAB [53]; GraSR [54] in Staphylococcus aureus ; PhoPQ in Escherichia coli [55]; BvrSR in Brucella abortus [56]; and PrrAB in Mycobacterium tuberculosis [57], among others. In S. pneumoniae , most of the TCSs are required for full virulence in animal models of infection [58, 59] and we have shown that two of these systems, StkP/ComE and CiaRH [15, 16], are important in response to acidic and/or oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

The pneumococcal two-component system VisRH is linked to enhanced intracellular survival ofStreptococcus pneumoniaein influenza-infected pneumocytes

Reinoso-Vizcaíno

¹

,

Cian

²

,

Cortes

³

et al. 2019

Preprint

0

View full text Add to dashboard Cite

The virus-bacterial synergism implicated in secondary bacterial infections caused by Streptococcus pneumoniae following infection with epidemic or pandemic influenza A virus (IAV) is well documented. However, the molecular mechanisms behind such synergism remain largely ill-defined. In pneumocytes infected with influenza A virus, subsequent infection with S. pneumoniae leads to enhanced pneumococcal intracellular survival. The pneumococcal two-component system VisRH appears essential for such enhanced survival. Through comparative transcriptomic analysis between the ΔvisR and wt strains, a list of 179 differentially expressed genes was defined. Among those, the clpL protein chaperone gene and the psaB Mn+2 transporter gene, which are involved in the stress response, are important in enhancing S. pneumoniae survival in influenza-infected cells. The ΔvisR, ΔclpL and ΔpsaB deletion mutants display increased susceptibility to acidic and oxidative stress and no enhancement of intracellular survival in IAV-infected pneumocyte cells. These results suggest that the VisRH two-component system senses IAV-induced stress conditions and controls adaptive responses that allow survival of S. pneumoniae in IAV-infected pneumocytes.Author summaryS. pneumoniae is an inhabitant of the human nasopharynx that is capable of causing a variety of infections contributing to an estimated 1.6 million deaths each year. Many of these deaths occur as result of secondary S. pneumoniae infections following seasonal or pandemic influenza. Although S. pneumoniae is considered a typical extracellular pathogen, an intracellular survival mechanism has been more recently recognized as significant in bacterial pathogenesis. The synergistic effects between influenza A and S. pneumoniae in secondary bacterial infection are well documented; however, the effects of influenza infections on intracellular survival of S. pneumoniae are ill-defined. Here, we provide evidence that influenza infection increases S. pneumoniae intracellular survival in pneumocytes. We demonstrate that the poorly understood VisRH signal transduction system in pneumococcus controls the expression of genes involved in the stress response that S. pneumoniae needs to increase intracellular survival in influenza A-infected pneumocytes. These findings have important implications for understanding secondary bacterial pathogenesis following influenza and for the treatment of such infections in influenza-stricken patients.

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LysR-type transcriptional regulators: state of the art

Mayo-Pérez,

Gama-Martínez,

Dávila

et al. 2023

Critical Reviews in Microbiology

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Correction: The ArcAB two-component regulatory system promotes resistance to reactive oxygen species and systemic infection by Salmonella Typhimurium

Cited by 4 publications

References 1 publication

The pneumococcal two-component system SirRH is linked to enhanced intracellular survival of Streptococcus pneumoniae in influenza-infected pulmonary cells

The pneumococcal two-component system SirRH is linked to enhanced intracellular survival of Streptococcus pneumoniae in influenza-infected pulmonary cells

The pneumococcal two-component system VisRH is linked to enhanced intracellular survival ofStreptococcus pneumoniaein influenza-infected pneumocytes

LysR-type transcriptional regulators: state of the art

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