Correction: Rzepecka-Stojko, A., et al. Polyphenols from Bee Pollen: Structure, Absorption, Metabolism and Biological Activity. Molecules 2015, 20, 21732–21749

Rzepecka‐Stojko, Anna; Stojko, Jerzy; Kurek-Górecka, Anna; Górecki, Michał; Kabała‐Dzik, Agata; Kubina, Robert; Moździerz, Aleksandra; Buszman, Ewa

doi:10.3390/molecules21020159

Cited by 4 publications

(13 citation statements)

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“…The composition of phenolic compounds in bee pollen collected under Polish pollen flow conditions has not been well recognised. Rzepecka-Stojko et al (2010, 2012 showed that their content depended on the method extraction of these compounds and bee pollen extract storage. These studies used the spectrophotometric method with Folin-Ciocalteu reagent to determine phenolic compounds in bee pollen extracts, but only total phenolic content was determined as it was impossible to identify individual compounds with this method.…”

Section: Discussionmentioning

confidence: 99%

“…The high correlation between the antioxidative properties and the content of phenolic compounds suggests the quantitative analysis of these compounds to be equally important. The analysis of total polyphenolic content commonly utilises the spectrophotometric method with Folin-Ciocalteau reagent (Carpes et al, 2007(Carpes et al, , 2009LeBlanc et al, 2009;Mărghitaş et al, 2009;Graikou et al, 2011;Freire et al, 2012;Prelipcean, 2012;Ulusoy & Kolayli, 2013), which was also used in Polish research on the efficiency of different methods of extraction of these compounds and on storing conditions of bee pollen loads extracts (Rzepecka-Stojko et al, 2010, 2012. The composition of phenolic compounds in bee pollen collected under Polish pollen flow conditions has not been well recognised due to a lack of data concerning qualitative and quantitative composition.…”

Section: Introductionmentioning

confidence: 99%

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Application of HPLC-DAD Technique for Determination of Phenolic Compounds in Bee Pollen Loads

Waś

Szczesna

Rybak-Chmielewska

et al. 2017

Journal of Apicultural Science

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A b s t r a c t A method was elaborated to determine phenolic compounds (vanillin, caffeic, p-coumaric and salicylic acids, and flavonoids: rutin, hesperetin, quercetin, pinocembrin, apigenin, kaempferol, isorhamnetin, chrysin, and acacetin) in bee pollen loads using highperformance liquid chromatography with a diode array detector (HPLC-DAD). Phenolic compounds from bee pollen were isolated on Cleanert C18-SPE columns (500 mg/6 mL, Agela Technologies). Polyphenols were identified by comparing the retention times and spectra of compounds found in pollen load samples with the ones of the standard mixture. Quantitative analysis was conducted using the external standard method. In addition, basic validation parameters for the method were determined. For the identified compounds (except for the salicylic acid), satisfactory (≥0.997) linear correlations were obtained. The elaborated method showed high repeatability and inter-laboratory reproducibility. Variability coeffcients of the majority of phenolic compounds did not exceed 10% in conditions of repeatability and inter-laboratory reproducibility, and for the total polyphenolic content they were 1.7 and 5.1%, respectively. The pollen load samples (n = 15) differed in qualitative and quantitative composition of the phenolic compounds. In all the samples, we identified the p-coumaric and salicylic acids and flavonoids rutin, hesperetin, and apigenin nevertheless, these compounds' contents significantly differed among individual samples. The total phenolic content in the tested samples of pollen loads ranged from 0.653 to 5.966 mg/100 g (on average 2.737 mg/100 g).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Application of HPLC-DAD Technique for Determination of Phenolic Compounds in Bee Pollen Loads

Waś

Szczesna

Rybak-Chmielewska

et al. 2017

Journal of Apicultural Science

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“…(1)(2)(3)(4)(5)(6). Bee bread has been used for many years in traditional medicine, supplementary nutrition and alternative diets, primary due to its nutritional properties and health benefits (6,7). Bee bread can be considered a food supplement due to a high content of nutrients (8).…”

Section: Introductionmentioning

confidence: 99%

“…The potential application of bee bread as a food and nutriceutical supplement mainly depends on its chemical composition which varies directly with the flora of a region, geographic origin, climatic conditions, soil type, the time and year of pollen collection by bees, bee species, method of obtaining bee bread (presence or absence of drying operation) and storage conditions (5,6,8). It was set up that polyphenols have a strong impact on the antioxidant activity of bee bread extracts (1)(2)(3)(5)(6)(7)(8)(9). Bee bread has antimicrobial, antioxidant, hepatoprotective, immunomodulating, anti-inflammatory and antiradiation activity, adaptogenic properties, etc.…”

Section: Introductionmentioning

confidence: 99%

“…Bee bread has antimicrobial, antioxidant, hepatoprotective, immunomodulating, anti-inflammatory and antiradiation activity, adaptogenic properties, etc. (1,2,7,10). It stimulates the protective forces of the human body, normalizes metabolism, has a positive influence on the liver, nervous and endocrine system functions, and enhances regeneration of tissues, physical and mental persistence of the human body (2,7,(9)(10)(11)(12)(13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

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Analytical Procedure Elaboration of Total Flavonoid Content Determination and Antimicrobial Activity of Bee Bread Extracts

Hudz¹,

Yezerska²,

Grygorieva³

et al. 2019

Acta Poloniae Pharmaceutica - Drug Research

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Sixteen ethanolic extracts were obtained from seven different bee bread samples. The total flavonoid content in the extracts was determined by aluminium-chloride method and was in the range of 8.3 mg/L ± 6.24% to 195.3 mg/L ± 1.35% and 28.8 mg/L ± 19.33% to 603.3 mg/L ± 4.64% with reference to quercetin and rutin, respectively. The relative standard deviations (RSD) for parallel measurements for the calibration curves of quercetin dehydrate and rutin trihydrate were in the range of 0.51% to 9.39% and 5.02% to 19.91%, respectively. The RSD for parallel measurements for the extracts with reference to quercetin dihydrate and rutin trihydrate were in the range of 0.23% to 11.64% and 4.64% to 19.33%, respectively. The total flavonoid content mainly depended on a ratio of bee bread to 50% ethanol and technology of obtaining bee bread (presence or absence of drying operation). The increase of bee bread in the solvent in 2 times yield an elevation of the total flavonoid content in approximately 1.5 times at similar maceration time (7 days). The significant differences between results were statistically confirmed. Antimicrobial activity was evaluated with disc diffusion method. The best antibacterial activity of the bee bread extracts was found against Bacillus cereus CCM 2010, Clostridium perfringens CCM 4435, and Staphylococcus aureus subsp. aureus CCM 4223. The activity of the bee bread extracts against Gram-negative bacteria, Aspergillus and Penicillium genera was lower with moderate anticandidal activity. The obtained results indicated that it was very important to employ extracts with a high content of bee bread in 50% ethanol (1 : 5, 1 : 10) during the development of bee bread extracts with the purpose of their potential usage in medical practice. According to the results of this study, bee bread is a product which is rich in flavonoids and with good antibacterial activity against Gram-positive bacteria and can be considered as a raw material for the development of diet supplements and antimicrobial medicinal products.

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Investigating the Antioxidant and Color Properties of Bee Pollens of Various Plant Sources

Végh

Sipiczki

Csóka

2023

Chemistry & Biodiversity

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In our study, Central and Eastern European bee pollens of different botanical origins were compared, based on their antioxidant and color properties. Total phenolic content (TPC), total flavonoid content (TFC), and in vitro antioxidant capacity (by FRAP, CUPRAC, ABTS * + and DPPH * assays) were determined spectrophotometrically. Besides, Relative Antioxidant Capacity Indexes (RACI) were calculated. CIELAB color parameters (L*, a*, b*, chroma) were determined by using a tristimulus-based instrument. Potential correlations between the investigated parameters were also identified. Based on the results of the preliminary study, ethanol:distilled water (60 : 40) was chosen as an extraction solvent. The total phenolic content of our samples ranged between 9.41 and 27.49 mg GAE/g dw. Pollens showed TFC:TPC ratios between 9 and 44 %. RACI values indicate that rapeseed (Brassica napus), traveller's joy (Clematis vitalba) and phacelia (Phacelia tanacetifolia) pollens have relatively high, while pollens of certain plants of the Asteraceae family possess low antioxidant potential. Antioxidant properties correlated significantly in most cases. RACI values showed strong positive correlation with each of the other antioxidant capacity parameters, suggesting that this approach is well applicable for comparing the antioxidant potential of bee pollens. No clear correlation was found between the antioxidant and color parameters.

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Correction: Rzepecka-Stojko, A., et al. Polyphenols from Bee Pollen: Structure, Absorption, Metabolism and Biological Activity. Molecules 2015, 20, 21732–21749

Cited by 4 publications

References 0 publications

Application of HPLC-DAD Technique for Determination of Phenolic Compounds in Bee Pollen Loads

Application of HPLC-DAD Technique for Determination of Phenolic Compounds in Bee Pollen Loads

Analytical Procedure Elaboration of Total Flavonoid Content Determination and Antimicrobial Activity of Bee Bread Extracts

Investigating the Antioxidant and Color Properties of Bee Pollens of Various Plant Sources

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