Correction of Luminescence Spectra and Calculation of Quantum Efficiencies Using Computer Techniques.

Zinc cytochrome c fluorescence as a probe for conformational changes in cytochrome c oxidase

“…The absorbance of 1 mM-quinine bisulphate exceeds 1, and consequently dilutions are necessary for accurate fluorescence measurements. The results reported in Table 1 were obtained with quinine sulphate (1 mg/l) as the standard [26]. Dilutions are unnecessary when using this standard.…”

“…The quantum yield of fluorescence of porphyrin cytochrome c and zinc cytochrome c were determined by the method of Parker & Rees [25], with the observed fluorescence being corrected for the variation of lamp output with wavelength. The standard used was quinine sulphate dissolved (1 mg/l) in distilled water; this compound has a quantum yield of 0.55 [26]. The protein solutions had absorbances 0.2-0.3 and were corrected for inner-filtering by using the equation: I = (I0 +Id) x anti-ln (A1+ A2/2) c is the corrected fluorescence intensity, I0 is the uncorrected fluorescence intensity, Id is a factor that corrects for dilution, and A1 and A2 are the absorbances at the excitation and emission wavelengths respectively.…”

Considerations on organic compounds in solution and inorganic ions in glasses as fluorescent Standard Reference Materials

“…benzene and naphthalene fluoresce in the UV, anthracene exhibits blue fluorescence, naphthacene fluoresces in the green, and pentacene in the red. Anthracene has been studied quite extensively in ethanol and benzene, and the quantum efficiencies reported from these studjes vary from 0.25 to 0.33 [3,18,21,23,43,57,[63][64][65][66][67].…”

Energy transfer and excited state lifetime of some anthracene laser dyes

“…where the indices s and r refer to sample and reference; respectively, the integrals over I represent the corrected uorescence peak area (by cut and weight) [11]; A is the absorbance at the excitation wavelength; and n the refractive index of the solvent. Fluorescence was collected at right angle to the exciting radiation ( ex =337 nm) and the absorption spectra were collected over the spectral range 300 -600 nm.…”