Considerations on organic compounds in solution and inorganic ions in glasses as fluorescent Standard Reference Materials

Abstract

The use of various organic co mpound s in soluti on and inorganic ions in glasses has• bee n investi • gated as poss ible fluoresce nce Standard Refere nce Mate rials. Emphasis was placed on meas uring physical and chemical paramete rs such as stability, reprodu cibilities of absorbance and fluorescence meas urements , re lative quantum effi ciencies as a fun ction of excitation wavelength , etc., for quinine d erivatives and selected organic co mpounds. A brief discussion is in cluded on th e use of rare earth and non• rare earth in organic ions in glasses as standards.

Characterization of Standard Reference Material 2942, Ce-ion-doped glass, spectral correction standard for UV fluorescence

“…Desirable characteristics of artifact standards for fluorescence have been specified [11][12][13]1] and metal-ion-doped glasses have been suggested previously as fluorescence standards [14,11,15]. Many of the radiometric characteristics of glasses can influence their effectiveness as standards, including absorbance, fluorescence anisotropy, temperature dependence of fluorescence intensity, fluorescence lifetime and photostability [9].…”

Characterization of Standard Reference Material 2941, uranyl-ion-doped glass, spectral correction standard for fluorescence

“…Uranium-containing materials and glasses have played important roles in the understanding of fluorescence and the development of measurement techniques based on fluorescence [11][12][13][14][15][16][17][18][19]. Uranium glasses have been suggested as fluorescence standards in the past [8,20,21]. The standardization of the fluorescence lifetimes of uranylion-containing species in aqueous solution has also been proposed [22].…”

“…Desirable characteristics of artifact standards for fluorescence have been specified previously [1,[8][9][10] and include that they be photostable, homogeneous, temperature and polarization independent, low concentration, traceable to a primary standard, supplied with certified values and uncertainties, have a large Stokes shift and a long shelf life. Uranium-containing materials and glasses have played important roles in the understanding of fluorescence and the development of measurement techniques based on fluorescence [11][12][13][14][15][16][17][18][19].…”

Characterization of Standard Reference Material 2940, Mn-ion-doped glass, spectral correction standard for fluorescence

“…Desirable characteristics of artifact standards for fluorescence have been specified [1,[12][13][14] and metal-ion-doped glasses have been suggested as fluorescence standards previously [12,15,16]. Many of the radiometric characteristics of glasses can influence their effectiveness as standards, including absorbance, fluorescence anisotropy, temperature dependence of fluorescence intensity, fluorescence lifetime and photostability [8].…”