Correction of blood coagulation dysfunction and anemia by supplementation of red blood cell suspension, fresh frozen plasma, and apheresis platelet: Results of in vitro hemodilution experiments

Li, Ling; Yang, Jiangcun; Sun, Yang; Dang, Qian-Li; Xu, Cuixiang; Ma, Ting; Ren, Jiangkang

doi:10.1016/j.jcrc.2014.09.019

Cited by 5 publications

(6 citation statements)

References 21 publications

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“…Based on our previous finding that suspended red blood cells (RBCs) in PLTs stored at 4°C have high activities, this study aimed to 1) conduct a systematic study of PLTs stored at 4 and 22°C to analyze the change in the PLT ultramicrostructure, PLT count, PLT metabolism, and PLT aggregation function between the two PLT storage groups; 2) analyze the degree of PLT clot formation and verify the feasibility of PLT storage at 4°C using an automatic biochemical analyzer, blood gas analyzer, PLT aggregator, flow cytometer, thromboelastography (TEG), and other tests; and 3) provide data to support or discourage the development of a technology suitable for PLT storage at 4°C and a type of PLT product that targets hemostasis.…”

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confidence: 99%

Evaluation of the advantages of platelet concentrates stored at 4°C versus 22°C

et al. 2017

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confidence: 99%

Evaluation of the advantages of platelet concentrates stored at 4°C versus 22°C

et al. 2017

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“…Based on the results of previous studies, [22–24] the correction effect of PLT preservation in cold storage in the in vitro model of thrombocytopenia [25] has been verified. Thromboelastography (TEG) indices and routine blood indices were used to evaluate blood coagulation function, anemia corrective effect, and PLT counts on an in vitro model of massive blood loss and thrombocytopenia (see experimental flow chart; Fig.…”

Section: Methodsmentioning

confidence: 92%

“…To ensure that the Hb levels, PLT counts, and blood clotting function after the dilution would be maintained within normal ranges [25,26] (for instance, Hb 60–80 g/L, PLT count of 50–75 × 10 9 /L, and TEG-MA >40 mm), we employed Hb, PLT count, R, and MA as the main indicators to assess the correction efficiency of blood loss models. We corrected the models of massive blood loss with RBCs, FFP, and PLT stored under different conditions, and found that the corrected PLT counts within 14 days of storage at 4°C showed correction efficiency.…”

Section: Discussionmentioning

confidence: 99%

“…A model of in vitro massive blood loss was established by the in vitro hemodilution method employed in our previous study. [25] Preparation of the in vitro saline hemodilution model and measurement of routine blood indices and coagulation indices were as follows: the blood simple was diluted with normal saline in ratios of 1:9.…”

Section: Methodsmentioning

confidence: 99%

“…The ratios and the amount of the added RBCs, plasma, and PLTs were selected based on our previous study. [25,26] The detailed experimental procedures are shown in Figure 2.…”

Section: Methodsmentioning

confidence: 99%

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Good hemostatic effect of platelets stored at 4°C in an in vitro model of massive blood loss and thrombocytopenia

Yang

Sun

et al. 2019

Medicine

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This study compared the corrective effects of storage of platelets at 4°C and at 22°C in an in vitro model of massive blood loss and thrombocytopenia to provide an experimental basis for the storage of platelets for clinical applications. In vitro model of massive blood loss and thrombocytopenia were constructed by the in vitro hemodilution method and cell washing method. Using storage of platelets at 4°C (1, 3, 5, 7, 10, 14 days) and at 22°C (1, 3, 5 days) to correct the coagulation condition of the different models, by thromboelastography and by routine blood indices. ①Platelets stored at 4°C (1, 3, 5,7, 10, 14 days) and at 22°C (1, 3, 5 days) to correct the in vitro model of massive blood loss. Platelet count results improved from 17 to 27 × 10 9 /L to greater than 120 × 10 9 /L for 4°C storage, and 20 to 27 × 10 9 /L to greater than 120 × 10 9 /L for 22°C storage. Thromboelastography maximum amplitude (TEG-MA) results improved from 8.8 to 15.4 mm to greater than 43 mm for 4°C storage, and 12.2 to 14.4 mm to greater than 44.8 mm for 22°C storage. Thromboelastography reaction time values decreased from 9.9–24.9 minutes to 3.8–5.5 minutes for 4°C storage, and 9.9–22.7 minutes to 4.3–4.5 minutes for 22°C storage. ②Platelets stored at 4°C (1, 3, 5,7, 10, 14 days) and at 22°C (1, 3, 5 days) to correct the in vitro model of thrombocytopenia. Platelet count results improved from 12 to 34 × 10 9 /L to greater than 99 × 10 9 /L for 4°C storage, and 12 to 34 × 10 9 /L to greater than 120 × 10 9 /L for 22°C storage. TEG-MA results improved from 21.4 to 32.1 mm to greater than 49.1 mm for 4°C storage, and 21.4 to 31.6 mm to greater than 50.5 mm for 22°C storage. Platelets stored at 4°C and 22°C have the same correcting effect for 1, 3, and 5 days. Platelets stored at 4°C for 7 to 14 days have similarly hemostatic effect on the in vitro model of massive blood loss and thrombocytopenia.

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Trauma Resuscitation Consideration: Sex Matters

Coleman

Moore

Samuels

et al. 2019

Journal of the American College of Surgeons

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Background-Sex dimorphisms in coagulation have been recognized but whole blood assessment of these dimorphisms and their relationship to outcomes in trauma have not been investigated. This study characterizes the viscoelastic hemostatic profile of severely injured patients by sex and examines how sex-specific coagulation differences affect clinical outcomes, specifically massive transfusion (MT) and death. We hypothesize that severely injured females are more hypercoagulable and thus, have lower rates of MT and mortality.Study Design-Hemostatic profiles and clinical outcomes from all trauma activation patients from two Level I trauma centers were examined with sex as an experimental variable. As part of a prospective study, whole blood was collected and thrombelastography (TEG) was performed. Coagulation profiles were compared between sexes and association with MT and mortality were examined. Poisson regression with robust standard errors was performed.Results-Overall, 464 patients (23% female) were included. By TEG, females had a more hypercoagulable profile, with a higher angle (clot propagation) and maximum amplitude (MA, clot strength). Females were less likely to present with hyperfibrinolysis or prolonged activating clotting time than males. In the setting of depressed clot strength (abnormal MA), female sex

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Correction of blood coagulation dysfunction and anemia by supplementation of red blood cell suspension, fresh frozen plasma, and apheresis platelet: Results of in vitro hemodilution experiments

Cited by 5 publications

References 21 publications

Evaluation of the advantages of platelet concentrates stored at 4°C versus 22°C

Evaluation of the advantages of platelet concentrates stored at 4°C versus 22°C

Good hemostatic effect of platelets stored at 4°C in an in vitro model of massive blood loss and thrombocytopenia

Trauma Resuscitation Consideration: Sex Matters

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