2018
DOI: 10.1371/journal.pone.0196444
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Correction: MeltMan: Optimization, Evaluation, and Universal Application of a qPCR System Integrating the TaqMan qPCR and Melting Analysis into a Single Assay

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“…For this method, it is advisable to employ multiple probes that are complementary to unique regions of SARS-CoV-2 RNA because relying on one probe could lead to false negatives originating from higher mutagenic rates of viral nucleic acids. Such false negatives constitute an important and frequently reported issue in diagnostic virology [ [58] , [59] , [60] ]. Employing several fluorescent probes also increases the sensitivity of detection.…”
Section: Viral Diagnosticsmentioning
confidence: 99%
“…For this method, it is advisable to employ multiple probes that are complementary to unique regions of SARS-CoV-2 RNA because relying on one probe could lead to false negatives originating from higher mutagenic rates of viral nucleic acids. Such false negatives constitute an important and frequently reported issue in diagnostic virology [ [58] , [59] , [60] ]. Employing several fluorescent probes also increases the sensitivity of detection.…”
Section: Viral Diagnosticsmentioning
confidence: 99%