Correction: Impedimetric measurement of DNA–DNA hybridisation using microelectrodes with different radii for detection of methicillin resistant Staphylococcus aureus (MRSA)

Li, Poh Quan; Piper, Andrew; Schmueser, Ilka; Mount, Andrew R.; Corrigan, Damion K.

doi:10.1039/c7an90048a

Cited by 10 publications

(7 citation statements)

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“…An array of eight gold disc-shaped microelectrodes was engraved on a silicon needle with an objective of real-time in-situ monitoring of IL-6 [100] ( Figure 4A). This approach was envisioned following the development of a robust microelectrode-based electrochemical sensing platform for chemical and DNA sensing applications [124][125][126][127]. Figure 3a shows the equivalent circuit formed at the electrode-electrolyte interface.…”

Section: Electrode Interfacementioning

confidence: 99%

“…Figure 3a shows the equivalent circuit formed at the electrode-electrolyte interface. The interfacial chemistry at the surface of electrode can be best explored by electrochemical impedance spectroscopy due to the associated sensitivity [127][128][129][130][131]. The capture antibody, immobilized through an organic linker at the Au disc electrode's surface, permits detection by increasing charge transfer resistance (R ct ).…”

Section: Electrode Interfacementioning

confidence: 99%

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Recent Advances in Electrochemical and Optical Biosensors Designed for Detection of Interleukin 6

Khan

Mujahid

2020

Sensors

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Interleukin 6 (IL-6), being a major component of homeostasis, immunomodulation, and hematopoiesis, manifests multiple pathological conditions when upregulated in response to viral, microbial, carcinogenic, or autoimmune stimuli. High fidelity immunosensors offer real-time monitoring of IL-6 and facilitate early prognosis of life-threatening diseases. Different approaches to augment robustness and enhance overall performance of biosensors have been demonstrated over the past few years. Electrochemical- and fluorescence-based detection methods with integrated electronics have been subjects of intensive research due to their ability to offer a better signal-to-noise ratio, high specificity, ultra-sensitivity, and wide dynamic range. In this review, the pleiotropic role of IL-6 and its clinical significance is discussed in detail, followed by detection schemes devised so far for their quantitative analysis. A critical review on underlying signal amplification strategies and performance of electrochemical and optical biosensors is presented. In conclusion, we discuss the reliability and feasibility of the proposed detection technologies for commercial applications.

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Section: Electrode Interfacementioning

confidence: 99%

Section: Electrode Interfacementioning

confidence: 99%

Recent Advances in Electrochemical and Optical Biosensors Designed for Detection of Interleukin 6

Khan

Mujahid

2020

Sensors

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“…Electrochemical DNA biosensors represent an exciting approach to the fast, low-cost detection of clinically important biomarkers [9,10] at the point of care [11]. A range of electrode materials and electrochemical measurement approaches have been employed for sensitive measurements (cyclic voltammetry [12], differential pulse voltammetry [13], square wave voltammetry [14] and electrochemical impedance spectroscopy [15]). The potential of electrochemical biosensors, once matured as a technology to provide efficient clinical workflows is immense.…”

Section: Introductionmentioning

confidence: 99%

Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids

et al. 2020

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It is well-known that two major issues, preventing improved outcomes from cancer are late diagnosis and the evolution of drug resistance during chemotherapy, therefore technologies that address these issues can have a transformative effect on healthcare workflows. In this work we present a simple, low-cost DNA biosensor that was developed specifically to detect mutations in a key oncogene (KRAS). The sensor employed was a screen-printed array of carbon electrodes, used to perform parallel measurements of DNA hybridisation. A DNA amplification reaction was developed with primers for mutant and wild type KRAS sequences which amplified target sequences from representative clinical samples to detectable levels in as few as twenty cycles. High levels of sensitivity were demonstrated alongside a clear exemplar of assay specificity by showing the mutant KRAS sequence was detectable against a significant background of wild type DNA following amplification and hybridisation on the sensor surface. The time to result was found to be 3.5 h with considerable potential for optimisation through assay integration. This quick and versatile biosensor has the potential to be deployed in a low-cost, point-of-care test where patients can be screened either for early diagnosis purposes or monitoring of response to therapy.

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“…EIS is a technique that can rapidly detect events such as DNA hybridization, and changes in the shape and structure of DNA, etc., at the surface of the working electrode [22,23]. EIS utilizes a redox probe at the surface of the working electrode and measures the probe's ability to undergo a redox reaction [16].…”

Section: Introductionmentioning

confidence: 99%

Development of Electrochemical DNA Biosensor for Equine Hindgut Acidosis Detection

Davies

Thomas

Rizwan

et al. 2021

Sensors

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The pH drop in the hindgut of the horse is caused by lactic acid-producing bacteria which are abundant when a horse’s feeding regime is excessively carbohydrate rich. This drop in pH below six causes hindgut acidosis and may lead to laminitis. Lactic acid-producing bacteria Streptococcus equinus and Mitsuokella jalaludinii have been found to produce high amounts of L-lactate and D-lactate, respectively. Early detection of increased levels of these bacteria could allow the horse owner to tailor the horse’s diet to avoid hindgut acidosis and subsequent laminitis. Therefore, 16s ribosomal ribonucleic acid (rRNA) sequences were identified and modified to obtain target single stranded deoxyribonucleic acid (DNA) from these bacteria. Complementary single stranded DNAs were designed from the modified target sequences to form capture probes. Binding between capture probe and target single stranded deoxyribonucleic acid (ssDNA) in solution has been studied by gel electrophoresis. Among pairs of different capture probes and target single stranded DNA, hybridization of Streptococcus equinus capture probe 1 (SECP1) and Streptococcus equinus target 1 (SET1) was portrayed as gel electrophoresis. Adsorptive stripping voltammetry was utilized to study the binding of thiol modified SECP1 over gold on glass substrates and these studies showed a consistent binding signal of thiol modified SECP1 and their hybridization with SET1 over the gold working electrode. Cyclic voltammetry and electrochemical impedance spectroscopy were employed to examine the binding of thiol modified SECP1 on the gold working electrode and hybridization of thiol modified SECP1 with the target single stranded DNA. Both demonstrated the gold working electrode surface was modified with a capture probe layer and hybridization of the thiol bound ssDNA probe with target DNA was indicated. Therefore, the proposed electrochemical biosensor has the potential to be used for the detection of the non-synthetic bacterial DNA target responsible for equine hindgut acidosis.

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Correction: Impedimetric measurement of DNA–DNA hybridisation using microelectrodes with different radii for detection of methicillin resistant Staphylococcus aureus (MRSA)

Abstract: Correction for ‘Impedimetric measurement of DNA–DNA hybridisation using microelectrodes with different radii for detection of methicillin resistant Staphylococcus aureus (MRSA)’ by Poh Quan Li et al., Analyst, 2017, 142, 1946–1952.

Cited by 10 publications

References 0 publications

Recent Advances in Electrochemical and Optical Biosensors Designed for Detection of Interleukin 6

Recent Advances in Electrochemical and Optical Biosensors Designed for Detection of Interleukin 6

Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids

Development of Electrochemical DNA Biosensor for Equine Hindgut Acidosis Detection

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