Correction for Quenching Associated with Liquid Scintillation Counting

The stimulation by thrombin of glucose oxidation in human platelets.

“…At low concentrations of glucose, glucose oxidation increased with increasing concentration ( Figure 3), but in the range from 1 to 20 (Table II, These results show that mannose, fructose, and glucose were each oxidized by platelets and that the oxidation of each was stimulated by thrombin. In particular, platelets exposed to thrombin increased their oxidation of glucose-1-_4C by 11.2 mumoles (Table I, column c), of mannose-1-14C by 11 mjumoles (Table III, column b), and of fructose-U-14C by 13 mfumoles (Table III, column b).…”

“…The least active samples had activities at least four times the background, and counting time was selected to yield at least 10,000 counts so that the standard error of the count was less than 1%o. Values for counts per minute were converted to disintegrations per minute by the channels ratio method (20). Each incubation was carried out in duplicate, and the results, which usually agreed within 5%o and never differed by more than 10%o, were averaged.…”

Incorporation of l-leucine-14C into immunoglobulins by jejunal biopsies of patients with celiac sprue and other gastrointestinal diseases

“…The dry precipitates were dissolved in 1 ml NCS solvent, and the NCS solution was dissolved in 13 ml of 0.435%o DPO and toluene in glass counting vials for determination of radioactivity in a liquid scintillation spectrometer. Values for counts per minute were converted to disintegrations per minute by the channels ratio method (14).…”

Pathways of fatty acid metabolism in human platelets

“…The samples were counted in an automatic Packard Tri-Carb liquid scintillation spectrometer, and quenching corrections were made by the channel ratio method (14).…”