Correction: Cytochrome P450 metabolic resistance (CYP6P9a) to pyrethroids imposes a fitness cost in the major African malaria vector Anopheles funestus

Tchouakui, Magellan; Miranda, Jacob Riveron; Mugenzi, Leon M. J.; Djonabaye, Doumani; Wondji, Murielle J.; Tchoupo, Micareme; Tchapga, Williams; Njiokou, Flobert; Wondji, Charles S.

doi:10.1038/s41437-020-00360-2

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The CYP6P9b induced a reduction in mosquitoes' fecundity and fertility as previously observed for CYP6P9a ( Tchouakui et al, 2020c ). The proportion of females that laid eggs was lower in the resistant and heterozygote genotypes than for susceptible and females with resistant genotype CYP6P9b -RR showed a significantly reduced number of eggs and larvae compared to females with the CYP6P9b -SS susceptible genotype.…”

Section: Discussionsupporting

confidence: 78%

“…The same mosquitoes previously used for the assessment of the fitness cost associated with CYP6P9a ( Tchouakui et al, 2020c ) were used in this study to firstly evaluate the fitness cost linked with CYP6P9b and then the cumulative effect of CYP6P9b and CYP6P9a . These mosquitoes are from a hybrid strain obtained from the crossing between two Anopheles funestus laboratory strains: FANG (susceptible) and FUMOZ-R (resistant).…”

Section: Methodsmentioning

confidence: 99%

“…These mosquitoes are from a hybrid strain obtained from the crossing between two Anopheles funestus laboratory strains: FANG (susceptible) and FUMOZ-R (resistant). Moreover, DNA from mosquitoes maintained in the insectary for 10 generations was used to genotype the CYP6P9b as previously done for CYP6P9a ( Tchouakui et al, 2020c ) to evaluate the reversal to susceptibility by monitoring the frequency of CYP6P9b -R resistant allele and combined genotypes from both genes over generations.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Combined over-expression of two cytochrome P450 genes exacerbates the fitness cost of pyrethroid resistance in the major African malaria vector Anopheles funestus

Tchouakui

Mugenzi

Wondji

et al. 2021

Pesticide Biochemistry and Physiology

Self Cite

View full text Add to dashboard Cite

Metabolic resistance driven by multiple P450 genes is worsening insecticide resistance in malaria vectors. However, it remains unclear whether such multiple over-expression imposes an additive fitness cost in the vectors. Here, we showed that two highly over-expressed P450 genes ( CYP6P9a and CYP6P9b ) combine to impose additive fitness costs in pyrethroid-resistant Anopheles funestus . Genotyping of the CYP6P9b resistance allele in hybrid mosquitoes from a pyrethroid-resistant FUMOZ-R and the susceptible FANG strains revealed that this gene imposes a fitness cost in resistant mosquitoes similar to CYP6P9a . Homozygote susceptible CYP6P9b_S (SS) significantly lay more eggs than the resistant (OR = 2.2, P = 0.04) and with greater hatching rate ( p < 0.04). Homozygote resistant larvae CYP6P9b_R (RR) developed significantly slower than homozygote susceptible from L1-L4 (χ 2 = 7.2; P = 0.007) with a late pupation observed for RR compared to both heterozygotes and homozygotes susceptible ( χ 2 = 11.17; P = 0.0008). No difference was observed between genotypes for adult longevity with no change in allele frequency and gene expression across the lifespan. Furthermore, we established that CYP6P9b combines with CYP6P9a to additively exacerbate the fitness cost of pyrethroid resistance with a greater reduction in fecundity/fertility and increased developmental time of double homozygote resistant mosquitoes. Moreover, an increased proportion of double homozygote susceptible individuals was noted over 10 generations in the insecticide-free environment (χ 2 = 6.3; P = 0.01) suggesting a reversal to susceptibility in the absence of selection. Such greater fitness cost imposed by multiple P450 genes shows that resistance management strategy based on rotation could help slow the spread of resistance.

show abstract

Section: Discussionsupporting

confidence: 78%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Combined over-expression of two cytochrome P450 genes exacerbates the fitness cost of pyrethroid resistance in the major African malaria vector Anopheles funestus

Tchouakui

Mugenzi

Wondji

et al. 2021

Pesticide Biochemistry and Physiology

Self Cite

View full text Add to dashboard Cite

show abstract

Influence of GST- and P450-based metabolic resistance to pyrethroids on blood feeding in the major African malaria vector Anopheles funestus

et al. 2020

Self Cite

View full text Add to dashboard Cite

Insecticide resistance genes are often associated with pleiotropic effects on various mosquito life-history traits. However, very little information is available on the impact of insecticide resistance on blood feeding process in mosquitoes. Here, using two recently detected DNA-based metabolic markers in the major malaria vector, An. funestus, we investigated how metabolic resistance genes could affect the blood meal intake. After allowing both the field F1 and lab F8 Anopheles funestus strains to feed on the human arm for 30 minutes, we assessed the association between key parameters of blood meal process including, probing time, feeding duration, blood feeding success, blood meal size, and markers of glutathione S-transferase (L119F-GSTe2) and cytochrome P450 (CYP6P9a_R)-mediated metabolic resistance. None of the parameters of blood meal process was associated with L119F-GSTe2 genotypes. By contrast, for CYP6P9a_R, homozygous resistant mosquitoes were significantly more able to blood-feed than homozygous susceptible (OR = 3.3; CI 95%: 1.4-7.7; P = 0.01) mosquitoes. Moreover, the volume of blood meal ingested by CYP6P9a-SS mosquitoes was lower than that of CYP6P9a-RS (P<0.004) and of CYP6P9a-RR (P<0.006). This suggests that CYP6P9a gene is inked with the feeding success and blood meal size of An. funestus. However, no correlation was found in the expression of CYP6P9a and that of genes encoding for salivary proteins involved in blood meal process. This study suggests that P450-based metabolic resistance may influence the blood feeding process of Anopheles funestus mosquito and consequently its ability to transmit malaria parasites.

show abstract

Correction: Cytochrome P450 metabolic resistance (CYP6P9a) to pyrethroids imposes a fitness cost in the major African malaria vector Anopheles funestus

Abstract: An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Cited by 2 publications

References 0 publications

Combined over-expression of two cytochrome P450 genes exacerbates the fitness cost of pyrethroid resistance in the major African malaria vector Anopheles funestus

Combined over-expression of two cytochrome P450 genes exacerbates the fitness cost of pyrethroid resistance in the major African malaria vector Anopheles funestus

Influence of GST- and P450-based metabolic resistance to pyrethroids on blood feeding in the major African malaria vector Anopheles funestus

Contact Info

Product

Resources

About