“…To examine whether the amino acid used to modify the resin support affects enzyme loading during immobilization, SDS-PAGE of the supernatant and elution from the whole process was carried out to analyze the protein content by using ImageJ. 39,40 We use the SDS-PAGE gray analysis method to detect the protein concentration. We use a known protein concentration as a standard for a relative emission intensity of 100%.…”
Section: One-step Purication and Immobilization Of Akr Using Cell Lymentioning
Stable immobilization of aldehyde ketone reductase mutants containing non-standard amino acids on an epoxy resin via strain-promoted alkyne–azide cycloaddition.
“…To examine whether the amino acid used to modify the resin support affects enzyme loading during immobilization, SDS-PAGE of the supernatant and elution from the whole process was carried out to analyze the protein content by using ImageJ. 39,40 We use the SDS-PAGE gray analysis method to detect the protein concentration. We use a known protein concentration as a standard for a relative emission intensity of 100%.…”
Section: One-step Purication and Immobilization Of Akr Using Cell Lymentioning
Stable immobilization of aldehyde ketone reductase mutants containing non-standard amino acids on an epoxy resin via strain-promoted alkyne–azide cycloaddition.
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