Corosolic acid inhibits the proliferation of osteosarcoma cells by inducing apoptosis

Abstract

Abstract. Corosolic acid (CRA), a pentacyclic triterpene isolated from medicinal herbs, has been reported to exhibit anticancer properties in several cancers. However, the anticancer activity of CRA in osteosarcoma cells is still unclear. In the present study, the inhibitory effect of CRA in osteosarcoma MG-63 cells was investigated, and the results revealed that CRA significantly inhibited the viability of MG-63 cells in a dose-and time-dependent manner. A typical apoptotic hallmark such as DNA ladder was detected by agarose gel electrophoresis following treatment with CRA. Further experiments demonstrated that CRA induced apoptosis of MG-63 cells by flow cytometry using propidium iodide and annexin V staining. In addition, it was observed that the apoptosis of MG-63 cells induced by CRA was closely associated with activation of caspase-3 and caspase-9, loss of mitochondrial membrane potential, and release of cytochrome c from mitochondria, suggesting that CRA may trigger the activation of the mitochondria-mediated apoptosis pathway. In addition, the inhibition of caspase activity attenuated the CRA-induced apoptosis of MG-63 cells, which further confirmed the role of the mitochondrial pathway in CRA-induced apoptosis. These results indicated that CRA could induce the apoptosis of osteosarcoma cells through activating the mitochondrial pathway, which provides an evidence that CRA may be a useful chemotherapeutic agent for osteosarcoma.

Corosolic Acid Induces Non-Apoptotic Cell Death through Generation of Lipid Reactive Oxygen Species Production in Human Renal Carcinoma Caki Cells

“…Because corosolic acid has an anti-cancer effect in various cancer cells [ 11 , 12 , 13 , 15 , 16 , 18 ], we examined whether corosolic acid induces cell death in renal carcinoma Caki cells. Corosolic acid decreased cell viability and increased cell cytotoxicity in a dose-dependent manner ( Figure 1 A,B).…”

Fabrication of Chitosan‐18β‐Glycyrrhetinic Acid Modified Titanium Implants with Nanorod Arrays for Suppression of Osteosarcoma Growth and Improvement of Osteoblasts Activity

“…It is well known that cell apoptosis or programmed death is one of the basic mechanisms that regulates cell growth and death, and plays an important role in maintaining tissue homeostasis . Therefore, the apoptosis of induced cell death is an effective way to inhibit tumor growth.…”

Review on Corosolic Acid:Based on Various Pharmaceutical Effects

“…Corosolic acid as a pentacyclic triterpenes significantly inhibited osteosarcoma of MG-63 cells in a dose-and time-dependent manner and this provides that corosolic acid may be useful chemotherpeutic material for osteosarcoma 34 .…”