Coronins: the return of the crown

Uetrecht, Andrea C.; Bear, James E.

doi:10.1016/j.tcb.2006.06.002

Cited by 161 publications

(169 citation statements)

References 37 publications

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“…The protein L-plastin also is associated with focal adhesions, and its elevation induces proliferation, tissue invasion, and loss of E-cadherin in colorectal tumors (36). In addition, because coronin inactivates the actin-related protein 2/3 complex, its increase promotes cell motility (37). Expression of coronin 1C correlates with malignancy in glioma cells (38).…”

Section: Discussionmentioning

confidence: 99%

Discovery and validation of colonic tumor-associated proteins via metabolic labeling and stable isotopic dilution

Huttlin

Chen²,

Barrett-Wilt³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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The unique biology of a neoplasm is reflected by its distinct molecular profile compared with normal tissue. To understand tumor development better, we have undertaken a quantitative proteomic search for abnormally expressed proteins in colonic tumors from Apc Min/؉ (Min) mice. By raising pairs of Min and wild-type mice on diets derived from natural-abundance or 15 Nlabeled algae, we used metabolic labeling to compare protein levels in colonic tumor versus normal tissue. Because metabolic labeling allows internal control throughout sample preparation and analysis, technical error is minimized as compared with in vitro labeling. Several proteins displayed altered expression, and a subset was validated via stable isotopic dilution using synthetic peptide standards. We also compared gene and protein expression among tumor and nontumor tissue, revealing limited correlation. This divergence was especially pronounced for species showing biological change, highlighting the complementary perspectives provided by transcriptomics and proteomics. Our work demonstrates the power of metabolic labeling combined with stable isotopic dilution as an integrated strategy for the identification and validation of differentially expressed proteins using rodent models of human disease.colon cancer ͉ min mouse ͉ proteomics ͉ differential mass spectrometry ͉ transcriptomics

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Section: Discussionmentioning

confidence: 99%

Discovery and validation of colonic tumor-associated proteins via metabolic labeling and stable isotopic dilution

Huttlin

Chen²,

Barrett-Wilt³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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“…A comparative analysis of the human breast tissue proteome by 2-D DIGE in combination with MS or MS/MS enabled us to identify 53 proteins that showed significant changes in expression levels. Among the upregulated proteins, coronin-1A, p34-Arc, and Wdr1, which contain consecutive WDrepeat motifs at their N-termini, are concerned with actin remodeling [25][26][27]. The upregulation of coronin-1A and p34-Arc levels was consistent with the increase in gene expression.…”

Section: Discussionmentioning

confidence: 56%

“…Coronin-1A is known to mediate actin dynamics in a variety of processes [26], and is found abundantly in lymphocytes and macrophages [27]. Coronins that bind to filamentous actin and Arp2/3 complex are involved in modulating actin dynamics [26,27].…”

Section: Actin Remodeling Proteins Are Upregulated In Cancer Tissuesmentioning

confidence: 99%

“…Coronins that bind to filamentous actin and Arp2/3 complex are involved in modulating actin dynamics [26,27]. Though little is yet known about its relation to cancer, the coronin family of proteins are essential in cell motility since the knockdown of coronin-1B by shRNA decreased the motility of Rat2 cells [28].…”

Section: Actin Remodeling Proteins Are Upregulated In Cancer Tissuesmentioning

confidence: 99%

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Proteomic analysis of breast cancer tissue reveals upregulation of actin‐remodeling proteins and its relevance to cancer invasiveness

Kim

Bae

Lee

et al. 2009

Proteomics Clinical Apps

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There is an emerging interest in protein expression profiling with the aim of identifying novel diagnostic markers and therapeutic targets in breast cancer. We analyzed breast cancer tissues by 2-D DIGE using a narrow range IPG strip (pH 5.5-6.7) after the immunodepletion of serum albumin and Ig. Sixty-three protein spots were detected with more than 61.8-fold differences (p ,0.05 for three technical replicates) from a set of tissue samples in which three tumor and three nontumor samples were randomly selected from six breast cancer subjects and pooled separately. Of these, 53 proteins were successfully identified by MS. Among the proteins whose levels were increased, we identified three novel WD-repeat-motifbearing proteins that have been known to be involved in actin remodeling: Arp2/3 complex subunit 2 (p34-Arc), coronin-1A and WD-repeat protein 1 (Wdr1). Significantly increased amounts of p34-Arc and coronin-1A in breast cancer were also shown by Western blot analysis of matched tumor and nontumor tissue samples (N = 11, p ,0.05), and were consistent with the mRNA levels retrieved from publicly available microarray databases. The siRNA knockdown of p34-Arc attenuated the invasion of SK-BR3 breast cancer cells into Matrigel. In contrast, the overexpression of coronin-1A increased this invasive activity. Taken together, the cellular levels of p34-Arc and coronin-1A were linked to cancer development and migration. The data obtained from the present study provides new insight into the management of breast cancer.

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“…So how does CORO2A trigger coregulator exchange? CORO2A belongs to the coronin family of evolutionary conserved actin-binding proteins [13] that contain a N-terminal basic signature motif involved in binding to actin, a central WD40-repeat domain forming a sevenbladed β-propeller, and a C-terminal coiled-coiled region. Whereas most coronins function in actin-polymerization linked to the cytoskeleton, CORO2A is a nuclear protein [7,11].…”

mentioning

confidence: 99%

New wrestling rules of anti-inflammatory transrepression by oxysterol receptor LXR revealed

Treuter

2011

Cell Res

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Coronins: the return of the crown

Cited by 161 publications

References 37 publications

Discovery and validation of colonic tumor-associated proteins via metabolic labeling and stable isotopic dilution

Discovery and validation of colonic tumor-associated proteins via metabolic labeling and stable isotopic dilution

Proteomic analysis of breast cancer tissue reveals upregulation of actin‐remodeling proteins and its relevance to cancer invasiveness

New wrestling rules of anti-inflammatory transrepression by oxysterol receptor LXR revealed

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