Corn (Maize)

Neuffer, M. G.; Coe, E. H.

doi:10.1007/978-1-4684-2994-7_1

Cited by 11 publications

(7 citation statements)

References 75 publications

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“…In humans, there is evidence for pericentric reductions but telomeric increases in exchange rates per nucleotide, compared with the intervening regions of chromosome arms (NIH/CEPH Collaborative Mapping Group, 1992). In plants, there is often strong centromeric and telomeric suppression of recombination, extending over large sections of the chromosomes (Neuffer & Coe, 1974;Tanksley etal. 1992).…”

Section: Dependence Of Background Selection Effects On Selection Coefmentioning

confidence: 99%

The effect of recombination on background selection

Nordborg¹,

Charlesworth²,

1996

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An approximate equation is derived, which predicts the effect on variability at a neutral locus of background selection due to a set of partly linked deleterious mutations. Random mating, multiplicative fitnesses, and sufficiently large population size that the selected loci are in mutation/selection equilibrium are assumed. Given these assumptions, the equation is valid for an arbitrary genetic map, and for an arbitrary distribution of selection coefficients across loci. Monte Carlo computer simulations show that the formula performs well for small population sizes under a wide range of conditions, and even seems to apply when there are epistatic fitness interactions among the selected loci. Failure occurred only with very weak selection and tight linkage. The formula is shown to imply that weakly selected mutations are more likely than strongly selected mutations to produce regional patterning of variability along a chromosome in response to local variation in recombination rates. Loci at the extreme tip of a chromosome experience a smaller effect of background selection than loci closer to the centre. It is shown that background selection can produce a considerable overall reduction in variation in organisms with small numbers of chromosomes and short maps, such as Drosophila. Large overall effects are less likely in species with higher levels of genetic recombination, such as mammals, although local reductions in regions of reduced recombination might be detectable.

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Section: Dependence Of Background Selection Effects On Selection Coefmentioning

confidence: 99%

The effect of recombination on background selection

Nordborg¹,

Charlesworth²,

1996

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“…Some of the pioneering studies on the nature of X‐ray radiation‐induced mutations have been conducted with maize male gametophytes (Stadler, 1939). Since then, procedures and methods for generating and studying induced mutations in maize have been investigated with particular emphasis on pollen mutagenesis (Neuffer, 1957; Amano & Smith, 1965; Mottinger, 1970). However, no systematic analysis of the optimal conditions for mutant induction, or the nature of the resultant mutations, has been conducted.…”

Section: Introductionmentioning

confidence: 99%

Molecular genetic analysis of pollen irradiation mutagenesis in Arabidopsis

2004

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Summary• Pollen γ -irradiation mutagenesis was systematically investigated to generate targeted mutations in Arabidopsis .• Irradiation effects on viability, germination and frequency of loci deletions were evaluated.• Mutation frequency increased with irradiation dose and varied depending upon pollen developmental stage. Meiosis was the most irradiation-sensitive stage, however, it did not equate to the highest mutation frequency. High frequencies of targeted mutations were obtained by irradiation from the second mitosis to mature pollen stages, using 400 -600 Gy. Targeted mutations could also be obtained using lower doses of γ -rays (e.g. 200 Gy) provided that pollen was irradiated at an earlier developmental stage. ms1ttg marker locus pseudo-dominants were used to verify the presence and size of the resultant deletions.• The results demonstrate that γ -irradiation of pollen is an efficient approach for generating deletions in the Arabidopsis genome. Pollen mutagenesis offers the possibility of combining single-cell selection procedures with the advantages of haploid systems, including the ability to treat large numbers of pollen grains, the absence of chimerism and direct expression of targeted alleles in the M 1 generation.

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“…The high percentage of abnormality was Due to the presence of A, B and R genome in 6x Triticale, we presume that the expression of the sphaerococcum effect in MT47 sph results of a new gene(s) localized outside the D genome and this new gene is not alle--lic to the recessive gene in 3D chromosome of T .. sphaerococcum Perc., described by Sears (21) and P.Rao (20).…”

Section: Resultsmentioning

confidence: 99%

“…They differ entirely by their genomes constitution (AABBRR/2D).Sears(21) by means of monosome analysis established the localization of the gene which controls the symptoms of the T.sphaerococcum Perc., as a homozygous infective recessive (zero allele) in 3D/XVI chromosome. Later by the telocentric method Prabhakara Rao(20) affirmed and specified the localizaDownloaded by [University of Illinois Chicago] at 13:12 13 October 2014 Ears from MT 47 Triticale a/ Control forn1; b/ Sphaerococcum type.tion of this gene in the 3D chromosome at 5,7cM of the centromere.…”

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confidence: 99%

Transposable Elements in Wheat and Triticale Sphaerococcum Mutant Forms

Georgiev

Dekova

Atanassov³

et al. 2000

Biotechnology & Biotechnological Equipment

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Sphaerococcum mutant forms obtained after EMS treatment in T.aestivum (AABBDD) and6x-Triticale (AABBRR/2D) were studied at genetical, cytogenetical and molecular level. The initial genetic grouping was based on the existence of two types of sphaerococcum mutation; stable and unstable. Transposable elements like AciDs from wheat and Triticale are described in both Sphaerococcum mutant forms. Cytogeneticaly detectable chromosomal rearrangements including the main briges were established only in unstable mutant forms. Southern hybridization analysis of the unstable mutant forms, 49L sph and 49L aest indicated the presence of AciDs-/ike transposable elements in their genomes. The stable mutant form MT47 sph possesses Ds-like transposable element. Ac was present in the genome of mutant forms of 613, 6512, 491202/S1, S.r2x 613 and the control form ofT. aestivum (S.ranozreika 2). These observations provide a basis for the isolation and molecular characterization of the sphaerococcum locus in wheat and Triticale.

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Corn (Maize)

Cited by 11 publications

References 75 publications

The effect of recombination on background selection

The effect of recombination on background selection

Molecular genetic analysis of pollen irradiation mutagenesis in Arabidopsis

Transposable Elements in Wheat and Triticale Sphaerococcum Mutant Forms

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