Corepressor MMTR/DMAP1 is an intrinsic negative regulator of CAK kinase to regulate cell cycle progression

Shin, June Ho; Kang, Ho Chul; Park, Yun Yeon; Ha, Dong-Soo; Choi, Youn Hee; Eum, Hea Young; Kang, Bong Gu; Chae, Ji Hyung; Shin, Incheol; Lee, Jae-Ho; Kim, Chul Geun

doi:10.1016/j.bbrc.2010.09.126

Cited by 4 publications

(9 citation statements)

References 11 publications

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“…This situation may account for the highest QsDMAP1 expression detected in low cork quality producers in which cork presents higher amounts of nail inclusions. A regression analysis with QsDMAP1 and QsMET1 expression failed to reveal a significant correlation between the expression of both genes which suggests that QsDMAP1 role in this cork oak tissue exceeds the function of interacting directly with DNMT1 during the S phase (Rountree et al 2000;Shin et al 2010).…”

Section: De Novo Methylation Is Essential During Cork Differentiationmentioning

confidence: 87%

“…Coworking with DNA methyltransferases are several other proteins such as Methyltransferase 1 Associated Protein 1 (DMAP1), which was found to be co-localized with MET1 on replication foci (Rountree et al 2000;Shin et al 2010). In addition to its function helping MET1 to methylate DNA, DMAP1 has a large range of other functions such as its association with histone deacetylases (Rountree et al 2000), cell cycle control (Shin et al 2010), and nucleic acid reparation (Negishi et al 2009).…”

Section: Communicated By D Nealementioning

confidence: 99%

“…In addition to its function helping MET1 to methylate DNA, DMAP1 has a large range of other functions such as its association with histone deacetylases (Rountree et al 2000), cell cycle control (Shin et al 2010), and nucleic acid reparation (Negishi et al 2009). …”

Section: Communicated By D Nealementioning

confidence: 99%

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Expression of DNA methyltransferases is involved in Quercus suber cork quality

Ramos¹,

Rocheta²,

Carvalho³

et al. 2013

Tree Genetics & Genomes

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Cork oak (Quercus suber ) is an important Portuguese species, mainly due to the economic value of the cork it produces. Cork results from phellogen, a meristematic tissue, which can locally produce lenticels or have discontinuities, originating "defects": pores and nail inclusions that are detrimental to cork industrial use. Epigenetic processes control plant development and its deregulation can lead to altered phenotypes; therefore, the study of epigenetic players in the phellogen is important to understand the emergence of cork's defects. DNA methyltransferases (DNMTs) and one protein associated to MET1 (DMAP1) were characterized in Q. suber, and their gene expression was analyzed in phellogen and contiguous differentiating cell layers of trees producing high and low quality cork, after the evaluation of their defects by physical and image analysis methods. All classes of DNMTs (MET, DRM, and CMT) with the respective canonical motifs were identified in Q. suber. The expression analyses of these genes showed that QsDRM2 was the most active methyltransferases in the cells analyzed, and that all the genes were differentially expressed in trees with distinct cork quality, with a tendency for higher expression levels in low quality producers. Interestingly, the global methylation level was higher in cells with low expression of DNA methyltransferases. A positive and significant correlation was obtained between QsDMAP1 gene expression and the percentage of cork defects. This work provides the first evidence that cork quality in Q. suber is likely influenced by epigenetic mechanisms.

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Section: De Novo Methylation Is Essential During Cork Differentiationmentioning

confidence: 87%

Section: Communicated By D Nealementioning

confidence: 99%

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Expression of DNA methyltransferases is involved in Quercus suber cork quality

Ramos¹,

Rocheta²,

Carvalho³

et al. 2013

Tree Genetics & Genomes

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“…DMAP1 is the human homologous of the Arabidopsis SWC4, which is a subunit of the chromatin remodeling complex SWR1C, and also a component of the NuA4 histone acetyltransferase complex, both involved in transcriptional regulation (Bieluszewski et al, 2015) by interacting with the promoters of target genes (Mozgová et al, 2015). SWC4 which has nucleosome acetyltransferase H4 activity (Doyon et al, 2004; Bieluszewski et al, 2015), is involved in several cellular processes such as, the regulation of mitotic cell cycle progression (Shin et al, 2010), DNA repair (Lee et al, 2010), and has been considered essential for plant development (Mozgová et al, 2015). The human DMAP1 is also a co-regulator that stimulates global maintenance of DNA methylation by co-working with MET1 (Rountree et al, 2000) at sites of double strand break repair (Negishi et al, 2009; Shin et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…SWC4 which has nucleosome acetyltransferase H4 activity (Doyon et al, 2004; Bieluszewski et al, 2015), is involved in several cellular processes such as, the regulation of mitotic cell cycle progression (Shin et al, 2010), DNA repair (Lee et al, 2010), and has been considered essential for plant development (Mozgová et al, 2015). The human DMAP1 is also a co-regulator that stimulates global maintenance of DNA methylation by co-working with MET1 (Rountree et al, 2000) at sites of double strand break repair (Negishi et al, 2009; Shin et al, 2010). The diverse functions of the DMAP1/SWC4 protein, as well as its involvement in two chromatin remodeling complexes associated with gene expression, should account for the expression detected in cork tissues during its differentiation, as already seen in traumatic corks (Ramos et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Cork Oak Young and Traumatic Periderms Show PCD Typical Chromatin Patterns but Different Chromatin-Modifying Genes Expression

et al. 2018

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Plants are subjected to adverse conditions being outer protective tissues fundamental to their survival. Tree stems are enveloped by a periderm made of cork cells, resulting from the activity of the meristem phellogen. DNA methylation and histone modifications have important roles in the regulation of plant cell differentiation. However, studies on its involvement in cork differentiation are scarce despite periderm importance. Cork oak periderm development was used as a model to study the formation and differentiation of secondary protective tissues, and their behavior after traumatic wounding (traumatic periderm). Nuclei structural changes, dynamics of DNA methylation, and posttranslational histone modifications were assessed in young and traumatic periderms, after cork harvesting. Lenticular phellogen producing atypical non-suberized cells that disaggregate and form pores was also studied, due to high impact for cork industrial uses. Immunolocalization of active and repressive marks, transcription analysis of the corresponding genes, and correlations between gene expression and cork porosity were investigated. During young periderm development, a reduction in nuclei area along with high levels of DNA methylation occurred throughout epidermis disruption. As cork cells became more differentiated, whole nuclei progressive chromatin condensation with accumulation in the nuclear periphery and increasing DNA methylation was observed. Lenticular cells nuclei were highly fragmented with faint 5-mC labeling. Phellogen nuclei were less methylated than in cork cells, and in lenticular phellogen were even lower. No significant differences were detected in H3K4me3 and H3K18ac signals between cork cells layers, although an increase in H3K4me3 signals was found from the phellogen to cork cells. Distinct gene expression patterns in young and traumatic periderms suggest that cork differentiation might be under specific silencing regulatory pathways. Significant correlations were found between QsMET1, QsMET2, and QsSUVH4 gene expression and cork porosity. This work evidences that DNA methylation and histone modifications play a role in cork differentiation and epidermis induced tension-stress. It also provides the first insights into chromatin dynamics during cork and lenticular cells differentiation pointing to a distinct type of remodeling associated with cell death.

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Differential DNA Methylation Patterns Are Related to Phellogen Origin and Quality of Quercus suber Cork

et al. 2017

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DNA methylation is thought to influence Quercus suber cork quality, which is the main constraint for its economic valorisation. However, a deep knowledge of the cytosine methylation patterns disclosing the epigenetic variability of trees with different cork quality types is totally missing. This study investigates the hypothesis that variations in DNA methylation contribute to differences in cork cellular characteristics directly related to original or traumatic phellogen activity. We used MSAPs (Methylation Sensitive Amplified Polymorphism) to assess DNA methylation patterns of cork and leaf tissues of Q. suber adult trees growing in three cork oak stands. The relationship between the detected polymorphisms and the diversity of cork quality traits was explored by a marker-trait analysis focusing on the most relevant quality characteristics. Populations differed widely in cork quality, but only slightly in degree of epigenetic differentiation. Four MSAP markers (1.3% of the total) were significantly associated with the most noteworthy quality traits: wood inclusions (nails) and porosity. This evidence supports the potential role of cytosine methylation in the modulation of differential phellogen activity either involved in localized cell death or in pore production, resulting in different cork qualities. Although, the underlying basis of the methylation polymorphism of loci affecting cork quality traits remain unclear, the disclosure of markers statistically associated with cork quality strengthens the potential role of DNA methylation in the regulation of these traits, namely at the phellogen level.

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Corepressor MMTR/DMAP1 is an intrinsic negative regulator of CAK kinase to regulate cell cycle progression

Cited by 4 publications

References 11 publications

Expression of DNA methyltransferases is involved in Quercus suber cork quality

Expression of DNA methyltransferases is involved in Quercus suber cork quality

Cork Oak Young and Traumatic Periderms Show PCD Typical Chromatin Patterns but Different Chromatin-Modifying Genes Expression

Differential DNA Methylation Patterns Are Related to Phellogen Origin and Quality of Quercus suber Cork

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